Theaflavin Ameliorates Cerebral Ischemia-Reperfusion Injury in Rats Through Its Anti-Inflammatory Effect and Modulation of STAT-1
Theaflavin, a major constituent of black tea, possesses biological functions such as the antioxidative, antiviral, and anti-inflammatory ones. The purpose of this study was to verify whether theaflavin reduces focal cerebral ischemia injury in a rat model of middle cerebral artery occlusion (MCAO)....
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2006-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/MI/2006/30490 |
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| author | Fei Cai Cai-Rong Li Ji-Liang Wu Jian-Guo Chen Chao Liu Qing Min Wei Yu Chang-Han Ouyang Jin-He Chen |
| author_facet | Fei Cai Cai-Rong Li Ji-Liang Wu Jian-Guo Chen Chao Liu Qing Min Wei Yu Chang-Han Ouyang Jin-He Chen |
| author_sort | Fei Cai |
| collection | DOAJ |
| description | Theaflavin, a major constituent of black tea, possesses biological functions such as the antioxidative, antiviral, and anti-inflammatory ones. The purpose of this study was to verify whether theaflavin reduces focal cerebral ischemia injury in a rat model of middle cerebral artery occlusion (MCAO). Male Sprague-Dawley rats were anesthetized and subjected to 2 hours of MCAO followed 24 hours reperfusion. Theaflavin administration (5, 10, and 20 mg/kg, IV) ameliorated infarct and edema volume. Theaflavin inhibited leukocyte infiltration and expression of ICAM-1, COX-2, and iNOS in injured brain. Phosphorylation of STAT-1, a protein which mediates intracellular signaling to the nucleus, was enhanced 2-fold over that of sham group and was inhibited by theaflavin. Our study demonstrated that theaflavin significantly protected neurons from cerebral ischemia-reperfusion injury by limiting leukocyte infiltration and expression of ICAM-1, and suppressing upregulation of inflammatory-related prooxidative enzymes (iNOS and COX-2) in ischemic brain via, at least in part, reducing the phosphorylation of STAT-1. |
| format | Article |
| id | doaj-art-7d9ce8009341418985545ab038b255b5 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-7d9ce8009341418985545ab038b255b52025-02-03T06:06:00ZengWileyMediators of Inflammation0962-93511466-18612006-01-01200610.1155/MI/2006/3049030490Theaflavin Ameliorates Cerebral Ischemia-Reperfusion Injury in Rats Through Its Anti-Inflammatory Effect and Modulation of STAT-1Fei Cai0Cai-Rong Li1Ji-Liang Wu2Jian-Guo Chen3Chao Liu4Qing Min5Wei Yu6Chang-Han Ouyang7Jin-He Chen8Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, ChinaDepartment of Pharmacology, Medical College, Xianning University, 3 Guihua Road, Xianning 437100, ChinaDepartment of Pharmacology, Medical College, Xianning University, 3 Guihua Road, Xianning 437100, ChinaDepartment of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, ChinaDepartment of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, ChinaDepartment of Pharmacology, Medical College, Xianning University, 3 Guihua Road, Xianning 437100, ChinaDepartment of Pharmacology, Medical College, Xianning University, 3 Guihua Road, Xianning 437100, ChinaDepartment of Pharmacology, Medical College, Xianning University, 3 Guihua Road, Xianning 437100, ChinaDepartment of Pharmacology, Medical College, Wuhan University, 39 Donghu Road, Wuhan 430071, ChinaTheaflavin, a major constituent of black tea, possesses biological functions such as the antioxidative, antiviral, and anti-inflammatory ones. The purpose of this study was to verify whether theaflavin reduces focal cerebral ischemia injury in a rat model of middle cerebral artery occlusion (MCAO). Male Sprague-Dawley rats were anesthetized and subjected to 2 hours of MCAO followed 24 hours reperfusion. Theaflavin administration (5, 10, and 20 mg/kg, IV) ameliorated infarct and edema volume. Theaflavin inhibited leukocyte infiltration and expression of ICAM-1, COX-2, and iNOS in injured brain. Phosphorylation of STAT-1, a protein which mediates intracellular signaling to the nucleus, was enhanced 2-fold over that of sham group and was inhibited by theaflavin. Our study demonstrated that theaflavin significantly protected neurons from cerebral ischemia-reperfusion injury by limiting leukocyte infiltration and expression of ICAM-1, and suppressing upregulation of inflammatory-related prooxidative enzymes (iNOS and COX-2) in ischemic brain via, at least in part, reducing the phosphorylation of STAT-1.http://dx.doi.org/10.1155/MI/2006/30490 |
| spellingShingle | Fei Cai Cai-Rong Li Ji-Liang Wu Jian-Guo Chen Chao Liu Qing Min Wei Yu Chang-Han Ouyang Jin-He Chen Theaflavin Ameliorates Cerebral Ischemia-Reperfusion Injury in Rats Through Its Anti-Inflammatory Effect and Modulation of STAT-1 Mediators of Inflammation |
| title | Theaflavin Ameliorates Cerebral Ischemia-Reperfusion Injury in Rats Through Its Anti-Inflammatory Effect and Modulation of STAT-1 |
| title_full | Theaflavin Ameliorates Cerebral Ischemia-Reperfusion Injury in Rats Through Its Anti-Inflammatory Effect and Modulation of STAT-1 |
| title_fullStr | Theaflavin Ameliorates Cerebral Ischemia-Reperfusion Injury in Rats Through Its Anti-Inflammatory Effect and Modulation of STAT-1 |
| title_full_unstemmed | Theaflavin Ameliorates Cerebral Ischemia-Reperfusion Injury in Rats Through Its Anti-Inflammatory Effect and Modulation of STAT-1 |
| title_short | Theaflavin Ameliorates Cerebral Ischemia-Reperfusion Injury in Rats Through Its Anti-Inflammatory Effect and Modulation of STAT-1 |
| title_sort | theaflavin ameliorates cerebral ischemia reperfusion injury in rats through its anti inflammatory effect and modulation of stat 1 |
| url | http://dx.doi.org/10.1155/MI/2006/30490 |
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