One Health Approach to Study the Occurrence and Antimicrobial Resistance of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella</i> spp. in Urban Agriculture in Burkina Faso

One Health Approach to Study the Occurrence and Antimicrobial Resistance of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella</i> spp. in Urban Agriculture in Burkina Faso

Data on antimicrobial resistance in Burkina Faso’s agricultural sector is still limited. This study assessed the occurrence of extended-spectrum β-lactamase-producing <i>Escherichia coli</i> (ESBL-<i>Ec</i>) and <i>Klebsiella</i> spp. (ESBL-<i>K</i>) i...

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Main Authors:	Fatimata Bintou Josiane Diarra, Isidore Juste Ouindgueta Bonkoungou, Zakaria Garba, Namwin Siourimè Somda, Djifahamaï Soma, Marguerite Edith Malatala Nikiema, Evariste Bako, Souleymane Sore, Natéwindé Sawadogo, Nicolas Barro, Kaisa Haukka
Format:	Article
Language:	English
Published:	MDPI AG 2024-10-01
Series:	Microorganisms
Subjects:	<i>Escherichia coli</i> <i>Klebsiella</i> spp. ESBL market gardens Burkina Faso
Online Access:	https://www.mdpi.com/2076-2607/12/11/2170
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Summary:	Data on antimicrobial resistance in Burkina Faso’s agricultural sector is still limited. This study assessed the occurrence of extended-spectrum β-lactamase-producing <i>Escherichia coli</i> (ESBL-<i>Ec</i>) and <i>Klebsiella</i> spp. (ESBL-<i>K</i>) in lettuce, environment, and gardeners’ stools in market gardens in Ouagadougou, Burkina Faso. A total of 356 samples were collected from three vegetable gardens (76 lettuce, 76 soil, 62 manure, 63 irrigation water, and 79 human stools). The ESBL-selective medium was used for initial selection of ESBL-producing bacteria, and the isolates were further identified using biochemical tests. An antibiotic susceptibility test was performed using the disk diffusion method. The overall prevalence of ESBL-<i>Ec</i> and/or ESBL-<i>K</i> in the samples was 232/356 (65.2%). Of the lettuce samples, the prevalence of ESBL-<i>Ec</i> was 19/76 (25.0%) and ESBL-<i>K</i> 33/76 (43.4%). In the market gardens environment, the prevalence of ESBL-<i>Ec</i> was 32/201 (15.9%) and ESBL-<i>K</i> 124/201 (61.7%). In the gardeners’ stools, the prevalence of ESBL-<i>Ec</i> was 42/79 (53.2%) and ESBL-<i>K</i> 24/79 (30.4%). Two ESBL-<i>K</i> isolates were found to produce NDM carbapenemase. Due to the high prevalence of ESBL-producing bacteria, which may also be carbapenemase producers, it is necessary to monitor pathogens in agricultural products with a “One Health” approach to limit and prevent infections in the population.
ISSN:	2076-2607