Microdifferential Pressure Measurement Device for Cellular Microenvironments
Mechanical forces influence cellular proliferation, differentiation, tissue morphogenesis, and functional expression within the body. To comprehend the impact of these forces on living organisms, their quantification is essential. This study introduces a novel microdifferential pressure measurement...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-12-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/12/1/3 |
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| author | Mami Akaike Jun Hatakeyama Yoichi Saito Yoshitaka Nakanishi Kenji Shimamura Yuta Nakashima |
| author_facet | Mami Akaike Jun Hatakeyama Yoichi Saito Yoshitaka Nakanishi Kenji Shimamura Yuta Nakashima |
| author_sort | Mami Akaike |
| collection | DOAJ |
| description | Mechanical forces influence cellular proliferation, differentiation, tissue morphogenesis, and functional expression within the body. To comprehend the impact of these forces on living organisms, their quantification is essential. This study introduces a novel microdifferential pressure measurement device tailored for cellular-scale pressure assessments. The device comprises a glass substrate and a microchannel constructed of polydimethylsiloxane, polytetrafluoroethylene tubes, a glass capillary, and a microsyringe pump. This device obviates the need for electrical measurements, relying solely on the displacement of ultrapure water within the microchannel to assess the micropressure in embryos. First, the device was subjected to arbitrary pressures, and the relationship between the pressure and the displacement of ultrapure water in the microchannel was determined. Calibration results showed that the displacement <i>dx</i> [μm] could be calculated from the pressure <i>P</i> [Pa] using the equation <i>dx</i> = 0.36 <i>P</i>. The coefficient of determination was shown to be 0.87, indicating a linear response. When utilized to measure brain ventricular pressure in mouse embryos, the fabricated device yielded an average pressure reading of 1313 ± 640 Pa. This device can facilitate the measurement of pressure within microcavities in living tissues and other areas requiring precise and localized pressure evaluations. |
| format | Article |
| id | doaj-art-7d828736a4a645b28778eaae5c66eb6f |
| institution | Kabale University |
| issn | 2306-5354 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj-art-7d828736a4a645b28778eaae5c66eb6f2025-01-24T13:22:55ZengMDPI AGBioengineering2306-53542024-12-01121310.3390/bioengineering12010003Microdifferential Pressure Measurement Device for Cellular MicroenvironmentsMami Akaike0Jun Hatakeyama1Yoichi Saito2Yoshitaka Nakanishi3Kenji Shimamura4Yuta Nakashima5Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, JapanInstitute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjyo, Chuo-ku, Kumamoto 860-0811, JapanFaculty of Advanced Science and Technology, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, JapanFaculty of Advanced Science and Technology, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, JapanInstitute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjyo, Chuo-ku, Kumamoto 860-0811, JapanFusion Oriented Research for Disruptive Science and Technology, Japan Science and Technology Agency, 5-3, Yonbancho, Chiyoda-ku, Tokyo 102-8666, JapanMechanical forces influence cellular proliferation, differentiation, tissue morphogenesis, and functional expression within the body. To comprehend the impact of these forces on living organisms, their quantification is essential. This study introduces a novel microdifferential pressure measurement device tailored for cellular-scale pressure assessments. The device comprises a glass substrate and a microchannel constructed of polydimethylsiloxane, polytetrafluoroethylene tubes, a glass capillary, and a microsyringe pump. This device obviates the need for electrical measurements, relying solely on the displacement of ultrapure water within the microchannel to assess the micropressure in embryos. First, the device was subjected to arbitrary pressures, and the relationship between the pressure and the displacement of ultrapure water in the microchannel was determined. Calibration results showed that the displacement <i>dx</i> [μm] could be calculated from the pressure <i>P</i> [Pa] using the equation <i>dx</i> = 0.36 <i>P</i>. The coefficient of determination was shown to be 0.87, indicating a linear response. When utilized to measure brain ventricular pressure in mouse embryos, the fabricated device yielded an average pressure reading of 1313 ± 640 Pa. This device can facilitate the measurement of pressure within microcavities in living tissues and other areas requiring precise and localized pressure evaluations.https://www.mdpi.com/2306-5354/12/1/3mechanical forcemicroenvironmentbrain pressureembryomicrodifferential pressure sensor |
| spellingShingle | Mami Akaike Jun Hatakeyama Yoichi Saito Yoshitaka Nakanishi Kenji Shimamura Yuta Nakashima Microdifferential Pressure Measurement Device for Cellular Microenvironments Bioengineering mechanical force microenvironment brain pressure embryo microdifferential pressure sensor |
| title | Microdifferential Pressure Measurement Device for Cellular Microenvironments |
| title_full | Microdifferential Pressure Measurement Device for Cellular Microenvironments |
| title_fullStr | Microdifferential Pressure Measurement Device for Cellular Microenvironments |
| title_full_unstemmed | Microdifferential Pressure Measurement Device for Cellular Microenvironments |
| title_short | Microdifferential Pressure Measurement Device for Cellular Microenvironments |
| title_sort | microdifferential pressure measurement device for cellular microenvironments |
| topic | mechanical force microenvironment brain pressure embryo microdifferential pressure sensor |
| url | https://www.mdpi.com/2306-5354/12/1/3 |
| work_keys_str_mv | AT mamiakaike microdifferentialpressuremeasurementdeviceforcellularmicroenvironments AT junhatakeyama microdifferentialpressuremeasurementdeviceforcellularmicroenvironments AT yoichisaito microdifferentialpressuremeasurementdeviceforcellularmicroenvironments AT yoshitakanakanishi microdifferentialpressuremeasurementdeviceforcellularmicroenvironments AT kenjishimamura microdifferentialpressuremeasurementdeviceforcellularmicroenvironments AT yutanakashima microdifferentialpressuremeasurementdeviceforcellularmicroenvironments |