Design of a Lab built Fully Automated Microfluidic Fluorometric System for Fluorescent Dye Applications
Microfluidics can process small amounts of fluids by using microscopic channels with microscale dimensions ranging from tens to hundreds of micrometers. Many researchers have recently been working on smaller analytical equipment, such as micro flow cytometers. These gadgets offer numerous advantages...
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National Centre of Excellence in Analytical Chemsitry
2025-06-01
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| Series: | Pakistan Journal of Analytical & Environmental Chemistry |
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| Online Access: | https://pjaec.pk/index.php/pjaec/article/view/2 |
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| author | Maitham Najim Aboud K. H. Al-Sowdani |
| author_facet | Maitham Najim Aboud K. H. Al-Sowdani |
| author_sort | Maitham Najim Aboud |
| collection | DOAJ |
| description | Microfluidics can process small amounts of fluids by using microscopic channels with microscale dimensions ranging from tens to hundreds of micrometers. Many researchers have recently been working on smaller analytical equipment, such as micro flow cytometers. These gadgets offer numerous advantages, including portability, low cost, and low power consumption. It can also be integrated with other microfluidic devices for multitasking applications. In this study, the fluorescein dye was estimated using a lab-builtfully automated microfluidic fluorometric system. The developed system contained a microfluidic chip with two lines, each four centimetres long and with a volume of 15 μL. In this setup, two Arduino UNO (R3 version) microcontrollers were programmed using homebrew software. The first was to manage the two improvised peristaltic
pumps, which successfully introduced the fluorescein dye to the water stream automatically, replacing the manual injection. The volume of the fluorescein sample was modified by varying the fluorescein line's run time. The second one was utilized to record the output signal. The method accurately identified fluorescein concentrations up to 0.01 μg/mL and 0.999 as a regression coefficient for six points. The RSD% (Relative Standard Deviation) for ten 0.08 μg/mL fluorescein measurements was 0%, and the detection limit was 1x10-4 μg/mL. The suggested method can measure 300 samples per hour while decreasing the amounts of reagents and waste.
This microfluidic fluorometric system has the potential to be used for fluorescent dye assays in a variety of applications. |
| format | Article |
| id | doaj-art-7d71005dfd4a4fad978dcf2adbef13e5 |
| institution | OA Journals |
| issn | 1996-918X 2221-5255 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | National Centre of Excellence in Analytical Chemsitry |
| record_format | Article |
| series | Pakistan Journal of Analytical & Environmental Chemistry |
| spelling | doaj-art-7d71005dfd4a4fad978dcf2adbef13e52025-08-20T02:08:11ZengNational Centre of Excellence in Analytical ChemsitryPakistan Journal of Analytical & Environmental Chemistry1996-918X2221-52552025-06-012501132110.21743/pjaec/2024.06.02Design of a Lab built Fully Automated Microfluidic Fluorometric System for Fluorescent Dye Applications Maitham Najim Aboud0K. H. Al-Sowdani 1Chemistry Department, College of Education for Pure Sciences, University of Basrah, Basrah, Iraq Chemistry Department, College of Education for Pure Sciences, University of Basrah, Basrah, Iraq Microfluidics can process small amounts of fluids by using microscopic channels with microscale dimensions ranging from tens to hundreds of micrometers. Many researchers have recently been working on smaller analytical equipment, such as micro flow cytometers. These gadgets offer numerous advantages, including portability, low cost, and low power consumption. It can also be integrated with other microfluidic devices for multitasking applications. In this study, the fluorescein dye was estimated using a lab-builtfully automated microfluidic fluorometric system. The developed system contained a microfluidic chip with two lines, each four centimetres long and with a volume of 15 μL. In this setup, two Arduino UNO (R3 version) microcontrollers were programmed using homebrew software. The first was to manage the two improvised peristaltic pumps, which successfully introduced the fluorescein dye to the water stream automatically, replacing the manual injection. The volume of the fluorescein sample was modified by varying the fluorescein line's run time. The second one was utilized to record the output signal. The method accurately identified fluorescein concentrations up to 0.01 μg/mL and 0.999 as a regression coefficient for six points. The RSD% (Relative Standard Deviation) for ten 0.08 μg/mL fluorescein measurements was 0%, and the detection limit was 1x10-4 μg/mL. The suggested method can measure 300 samples per hour while decreasing the amounts of reagents and waste. This microfluidic fluorometric system has the potential to be used for fluorescent dye assays in a variety of applications. https://pjaec.pk/index.php/pjaec/article/view/2automation instrumentsmicrofluidic applicationslab-made microfluidicfluorometric system |
| spellingShingle | Maitham Najim Aboud K. H. Al-Sowdani Design of a Lab built Fully Automated Microfluidic Fluorometric System for Fluorescent Dye Applications Pakistan Journal of Analytical & Environmental Chemistry automation instruments microfluidic applications lab-made microfluidic fluorometric system |
| title | Design of a Lab built Fully Automated Microfluidic Fluorometric System for Fluorescent Dye Applications |
| title_full | Design of a Lab built Fully Automated Microfluidic Fluorometric System for Fluorescent Dye Applications |
| title_fullStr | Design of a Lab built Fully Automated Microfluidic Fluorometric System for Fluorescent Dye Applications |
| title_full_unstemmed | Design of a Lab built Fully Automated Microfluidic Fluorometric System for Fluorescent Dye Applications |
| title_short | Design of a Lab built Fully Automated Microfluidic Fluorometric System for Fluorescent Dye Applications |
| title_sort | design of a lab built fully automated microfluidic fluorometric system for fluorescent dye applications |
| topic | automation instruments microfluidic applications lab-made microfluidic fluorometric system |
| url | https://pjaec.pk/index.php/pjaec/article/view/2 |
| work_keys_str_mv | AT maithamnajimaboud designofalabbuiltfullyautomatedmicrofluidicfluorometricsystemforfluorescentdyeapplications AT khalsowdani designofalabbuiltfullyautomatedmicrofluidicfluorometricsystemforfluorescentdyeapplications |