Programmed cell death-related gene IL20RA facilitates tumor progression and remodels tumor microenvironment in thyroid cancer
Abstract Programmed cell death (PCD) is a vital biological process that is essential for regulating cell progression and tumor microenvironment. This study aimed to explore the relationship between PCD-related genes expression and prognosis in thyroid cancer (THCA), especially IL20RA, as a potential...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-87059-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832571866532806656 |
|---|---|
| author | Yuqi Wang Yunlong Zhang Min Liu Yu Liu Yu Zeng Wei Zhang Shuping Wu Linfei Hu Xianhui Ruan Xiangqian Zheng Ming Gao Jingzhu Zhao |
| author_facet | Yuqi Wang Yunlong Zhang Min Liu Yu Liu Yu Zeng Wei Zhang Shuping Wu Linfei Hu Xianhui Ruan Xiangqian Zheng Ming Gao Jingzhu Zhao |
| author_sort | Yuqi Wang |
| collection | DOAJ |
| description | Abstract Programmed cell death (PCD) is a vital biological process that is essential for regulating cell progression and tumor microenvironment. This study aimed to explore the relationship between PCD-related genes expression and prognosis in thyroid cancer (THCA), especially IL20RA, as a potential prognostic marker for THCA. Data from The Cancer Genome Atlas (TCGA) database was utilized to develop a PCD-related risk prediction model based on LASSO regression along with univariate Cox regression. The correlation between PCD-related genes and immune cell infiltration was also assessed. The prognostic value of the key PCD-related gene for THCA was investigated by immunohistochemistry. The immune regulatory function and biological function of the key PCD related molecules were detected by cellular experiments. We identified four PCD-related genes (NPC2, E2F1, IL20RA, and TREM2) and constructed a risk model that exhibited excellent accuracy in predicting the prognosis of THCA. Moreover, we confirmed that high expression of IL20RA related to the poor prognosis of THCA. IL20RA promoted cell proliferation and IL20RA knockdown increased apoptosis and ferroptosis. Analysis of the immune microenvironment and detection of macrophages polarization showed that IL20RA promoted the polarization of M2 macrophages while reducing the polarization of M1. We constructed a prognostic prediction model and identified several PCD-related genes. The function of IL20RA which could potentially provide a foundation for additional investigations into diagnostic markers and treatment targets for THCA. |
| format | Article |
| id | doaj-art-7d5080894b10495ca986d7d4900a0aa0 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-7d5080894b10495ca986d7d4900a0aa02025-02-02T12:15:49ZengNature PortfolioScientific Reports2045-23222025-01-0115111510.1038/s41598-025-87059-8Programmed cell death-related gene IL20RA facilitates tumor progression and remodels tumor microenvironment in thyroid cancerYuqi Wang0Yunlong Zhang1Min Liu2Yu Liu3Yu Zeng4Wei Zhang5Shuping Wu6Linfei Hu7Xianhui Ruan8Xiangqian Zheng9Ming Gao10Jingzhu Zhao11Department of Thyroid and Neck Tumor, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and TherapyTianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and TherapyDepartment of Thyroid and Neck Tumor, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and TherapyDepartment of Thyroid and Neck Tumor, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and TherapyDepartment of Thyroid and Neck Tumor, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and TherapyDepartment of Thyroid and Breast Surgery, Tianjin Key Laboratory of General Surgery in Construction, Tianjin Union Medical CenterDepartment of Thyroid and Neck Tumor, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and TherapyDepartment of Thyroid and Neck Tumor, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and TherapyDepartment of Thyroid and Neck Tumor, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and TherapyDepartment of Thyroid and Neck Tumor, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and TherapyDepartment of Thyroid and Neck Tumor, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and TherapyDepartment of Thyroid and Neck Tumor, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and TherapyAbstract Programmed cell death (PCD) is a vital biological process that is essential for regulating cell progression and tumor microenvironment. This study aimed to explore the relationship between PCD-related genes expression and prognosis in thyroid cancer (THCA), especially IL20RA, as a potential prognostic marker for THCA. Data from The Cancer Genome Atlas (TCGA) database was utilized to develop a PCD-related risk prediction model based on LASSO regression along with univariate Cox regression. The correlation between PCD-related genes and immune cell infiltration was also assessed. The prognostic value of the key PCD-related gene for THCA was investigated by immunohistochemistry. The immune regulatory function and biological function of the key PCD related molecules were detected by cellular experiments. We identified four PCD-related genes (NPC2, E2F1, IL20RA, and TREM2) and constructed a risk model that exhibited excellent accuracy in predicting the prognosis of THCA. Moreover, we confirmed that high expression of IL20RA related to the poor prognosis of THCA. IL20RA promoted cell proliferation and IL20RA knockdown increased apoptosis and ferroptosis. Analysis of the immune microenvironment and detection of macrophages polarization showed that IL20RA promoted the polarization of M2 macrophages while reducing the polarization of M1. We constructed a prognostic prediction model and identified several PCD-related genes. The function of IL20RA which could potentially provide a foundation for additional investigations into diagnostic markers and treatment targets for THCA.https://doi.org/10.1038/s41598-025-87059-8Programmed cell deathThyroid cancerIL20RAPrognosisTumor microenvironment |
| spellingShingle | Yuqi Wang Yunlong Zhang Min Liu Yu Liu Yu Zeng Wei Zhang Shuping Wu Linfei Hu Xianhui Ruan Xiangqian Zheng Ming Gao Jingzhu Zhao Programmed cell death-related gene IL20RA facilitates tumor progression and remodels tumor microenvironment in thyroid cancer Scientific Reports Programmed cell death Thyroid cancer IL20RA Prognosis Tumor microenvironment |
| title | Programmed cell death-related gene IL20RA facilitates tumor progression and remodels tumor microenvironment in thyroid cancer |
| title_full | Programmed cell death-related gene IL20RA facilitates tumor progression and remodels tumor microenvironment in thyroid cancer |
| title_fullStr | Programmed cell death-related gene IL20RA facilitates tumor progression and remodels tumor microenvironment in thyroid cancer |
| title_full_unstemmed | Programmed cell death-related gene IL20RA facilitates tumor progression and remodels tumor microenvironment in thyroid cancer |
| title_short | Programmed cell death-related gene IL20RA facilitates tumor progression and remodels tumor microenvironment in thyroid cancer |
| title_sort | programmed cell death related gene il20ra facilitates tumor progression and remodels tumor microenvironment in thyroid cancer |
| topic | Programmed cell death Thyroid cancer IL20RA Prognosis Tumor microenvironment |
| url | https://doi.org/10.1038/s41598-025-87059-8 |
| work_keys_str_mv | AT yuqiwang programmedcelldeathrelatedgeneil20rafacilitatestumorprogressionandremodelstumormicroenvironmentinthyroidcancer AT yunlongzhang programmedcelldeathrelatedgeneil20rafacilitatestumorprogressionandremodelstumormicroenvironmentinthyroidcancer AT minliu programmedcelldeathrelatedgeneil20rafacilitatestumorprogressionandremodelstumormicroenvironmentinthyroidcancer AT yuliu programmedcelldeathrelatedgeneil20rafacilitatestumorprogressionandremodelstumormicroenvironmentinthyroidcancer AT yuzeng programmedcelldeathrelatedgeneil20rafacilitatestumorprogressionandremodelstumormicroenvironmentinthyroidcancer AT weizhang programmedcelldeathrelatedgeneil20rafacilitatestumorprogressionandremodelstumormicroenvironmentinthyroidcancer AT shupingwu programmedcelldeathrelatedgeneil20rafacilitatestumorprogressionandremodelstumormicroenvironmentinthyroidcancer AT linfeihu programmedcelldeathrelatedgeneil20rafacilitatestumorprogressionandremodelstumormicroenvironmentinthyroidcancer AT xianhuiruan programmedcelldeathrelatedgeneil20rafacilitatestumorprogressionandremodelstumormicroenvironmentinthyroidcancer AT xiangqianzheng programmedcelldeathrelatedgeneil20rafacilitatestumorprogressionandremodelstumormicroenvironmentinthyroidcancer AT minggao programmedcelldeathrelatedgeneil20rafacilitatestumorprogressionandremodelstumormicroenvironmentinthyroidcancer AT jingzhuzhao programmedcelldeathrelatedgeneil20rafacilitatestumorprogressionandremodelstumormicroenvironmentinthyroidcancer |