Reconstruction of experimentally induced radial bone defect in rabbits by using acellular fish swim bladder and autologous bone marrow clot: Histopathological assessment
In the current study, forty-five clinically healthy adult male rabbits, 9-12 months old, weighed 1.5-1.7 kg, were used. The experimental animals were randomly divided into three equal groups of 15. In all animals, segmental defects of 5 mm were induced in the midshaft of the radius. The defects were...
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University of Mosul, College of Veterinary Medicine
2025-01-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| author | Noor A. Mahdi Nadia H. Rija |
| author_facet | Noor A. Mahdi Nadia H. Rija |
| author_sort | Noor A. Mahdi |
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| description | In the current study, forty-five clinically healthy adult male rabbits, 9-12 months old, weighed 1.5-1.7 kg, were used. The experimental animals were randomly divided into three equal groups of 15. In all animals, segmental defects of 5 mm were induced in the midshaft of the radius. The defects were left without additives in the control group (CG). In the fish swim bladder treated group (FSB group), the created bone defects were wrapped using a previously prepared acellular fish swim bladder (FSB) and sutured around the bone defects. In the fish swim bladder and autologous bone marrow clot-treated group (FSB-BMC group), the defects were wrapped using FSB as described, with a portion of the aspirated 1 ml bone marrow clot filling each bone defect. The bone defects in all groups were evaluated histopathologically at 4,8- and 12-weeks post-operation to observe the bone formation and bridging of segmental bone defects. The results showed a significant increase in osteoblasts, osteocytes, Haversian canals, neovascularization blood vessels, and bone tissue formation in the FSB-BMC treated group compared to other groups at 4 weeks post-operation. At 8- and 12-weeks post-surgery, both treated groups showed a significant increase in osteoblasts, osteocytes, mature bone, bone trabeculae, Haversian canals and bone tissue compared to the control group. In conclusion, the FSB-BMC treated group showed a higher new bone formation and complete bridging than other groups. In contrast, the FSB-treated group showed new bone formation and partial bridging compared to the control group. |
| format | Article |
| id | doaj-art-7d3c9fcea1fd48beab14e60ecd9aba9d |
| institution | Kabale University |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| publishDate | 2025-01-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj-art-7d3c9fcea1fd48beab14e60ecd9aba9d2025-02-04T13:11:43ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552025-01-0139114315410.33899/ijvs.2024.154298.3937186168Reconstruction of experimentally induced radial bone defect in rabbits by using acellular fish swim bladder and autologous bone marrow clot: Histopathological assessmentNoor A. Mahdi0Nadia H. Rija1Department of Veterinary Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Baghdad, IraqDepartment of Veterinary Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Baghdad, IraqIn the current study, forty-five clinically healthy adult male rabbits, 9-12 months old, weighed 1.5-1.7 kg, were used. The experimental animals were randomly divided into three equal groups of 15. In all animals, segmental defects of 5 mm were induced in the midshaft of the radius. The defects were left without additives in the control group (CG). In the fish swim bladder treated group (FSB group), the created bone defects were wrapped using a previously prepared acellular fish swim bladder (FSB) and sutured around the bone defects. In the fish swim bladder and autologous bone marrow clot-treated group (FSB-BMC group), the defects were wrapped using FSB as described, with a portion of the aspirated 1 ml bone marrow clot filling each bone defect. The bone defects in all groups were evaluated histopathologically at 4,8- and 12-weeks post-operation to observe the bone formation and bridging of segmental bone defects. The results showed a significant increase in osteoblasts, osteocytes, Haversian canals, neovascularization blood vessels, and bone tissue formation in the FSB-BMC treated group compared to other groups at 4 weeks post-operation. At 8- and 12-weeks post-surgery, both treated groups showed a significant increase in osteoblasts, osteocytes, mature bone, bone trabeculae, Haversian canals and bone tissue compared to the control group. In conclusion, the FSB-BMC treated group showed a higher new bone formation and complete bridging than other groups. In contrast, the FSB-treated group showed new bone formation and partial bridging compared to the control group.https://www.vetmedmosul.com/article_186168_864135ea1734457d995d98d582d4f422.pdfsegmental bone defectfish swim bladderbone marrow clotbone regenerationrabbits |
| spellingShingle | Noor A. Mahdi Nadia H. Rija Reconstruction of experimentally induced radial bone defect in rabbits by using acellular fish swim bladder and autologous bone marrow clot: Histopathological assessment Iraqi Journal of Veterinary Sciences segmental bone defect fish swim bladder bone marrow clot bone regeneration rabbits |
| title | Reconstruction of experimentally induced radial bone defect in rabbits by using acellular fish swim bladder and autologous bone marrow clot: Histopathological assessment |
| title_full | Reconstruction of experimentally induced radial bone defect in rabbits by using acellular fish swim bladder and autologous bone marrow clot: Histopathological assessment |
| title_fullStr | Reconstruction of experimentally induced radial bone defect in rabbits by using acellular fish swim bladder and autologous bone marrow clot: Histopathological assessment |
| title_full_unstemmed | Reconstruction of experimentally induced radial bone defect in rabbits by using acellular fish swim bladder and autologous bone marrow clot: Histopathological assessment |
| title_short | Reconstruction of experimentally induced radial bone defect in rabbits by using acellular fish swim bladder and autologous bone marrow clot: Histopathological assessment |
| title_sort | reconstruction of experimentally induced radial bone defect in rabbits by using acellular fish swim bladder and autologous bone marrow clot histopathological assessment |
| topic | segmental bone defect fish swim bladder bone marrow clot bone regeneration rabbits |
| url | https://www.vetmedmosul.com/article_186168_864135ea1734457d995d98d582d4f422.pdf |
| work_keys_str_mv | AT nooramahdi reconstructionofexperimentallyinducedradialbonedefectinrabbitsbyusingacellularfishswimbladderandautologousbonemarrowclothistopathologicalassessment AT nadiahrija reconstructionofexperimentallyinducedradialbonedefectinrabbitsbyusingacellularfishswimbladderandautologousbonemarrowclothistopathologicalassessment |