Well-Characterized Polyethyleneimine-/Carboxylated-Polyethylene-Glycol-Functionalized Gold Nanoparticles as Prospective Nanoscale Control Materials for In Vitro Cell Viability Assays: Particle Characterization and Toxicity Tests in Eight Mammalian Cell Lines
The safety screening of manufactured nanomaterials (MNMs) is essential for their adoption by consumers and the marketplace. Lately, animal-based testing has been replaced by mechanistically informative in vitro assays due to the requirements of regulatory agencies. Cell viability assays are widely e...
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2025-01-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/15/2/79 |
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| author | Vytas Reipa Vincent A. Hackley Alessandro Tona Min Beom Heo Ye Ryeong Lee Tae Geol Lee Aaron Johnston-Peck Tae Joon Cho |
| author_facet | Vytas Reipa Vincent A. Hackley Alessandro Tona Min Beom Heo Ye Ryeong Lee Tae Geol Lee Aaron Johnston-Peck Tae Joon Cho |
| author_sort | Vytas Reipa |
| collection | DOAJ |
| description | The safety screening of manufactured nanomaterials (MNMs) is essential for their adoption by consumers and the marketplace. Lately, animal-based testing has been replaced by mechanistically informative in vitro assays due to the requirements of regulatory agencies. Cell viability assays are widely employed for manufactured nanomaterial hazard screening as a first-tier approach. Critical parts of such assays are positive and negative controls that serve as measurement benchmarks. We present the cellular viability and corresponding particle characterization obtained with eight different cell lines that were exposed to Au-PEI and Au-PEG-COOH nanoparticles. We showed that polyethyleneimine- and carboxylate-polyethylene-glycol-conjugated gold nanoparticles (AuPEI and Au-PEG-COOH) qualified for positive and negative controls in the in vitro cell viability assays used for MNM toxicological screening. |
| format | Article |
| id | doaj-art-7d1fdd0eac864309a540e2422db99a19 |
| institution | Kabale University |
| issn | 2079-4991 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj-art-7d1fdd0eac864309a540e2422db99a192025-01-24T13:44:03ZengMDPI AGNanomaterials2079-49912025-01-011527910.3390/nano15020079Well-Characterized Polyethyleneimine-/Carboxylated-Polyethylene-Glycol-Functionalized Gold Nanoparticles as Prospective Nanoscale Control Materials for In Vitro Cell Viability Assays: Particle Characterization and Toxicity Tests in Eight Mammalian Cell LinesVytas Reipa0Vincent A. Hackley1Alessandro Tona2Min Beom Heo3Ye Ryeong Lee4Tae Geol Lee5Aaron Johnston-Peck6Tae Joon Cho7Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USAMaterials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USAMaterials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USADivision of Biomedical Metrology, Korea Research Institute of Standards and Science, Daejeon 34113, Republic of KoreaDivision of Biomedical Metrology, Korea Research Institute of Standards and Science, Daejeon 34113, Republic of KoreaDivision of Biomedical Metrology, Korea Research Institute of Standards and Science, Daejeon 34113, Republic of KoreaMaterials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USAMaterials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USAThe safety screening of manufactured nanomaterials (MNMs) is essential for their adoption by consumers and the marketplace. Lately, animal-based testing has been replaced by mechanistically informative in vitro assays due to the requirements of regulatory agencies. Cell viability assays are widely employed for manufactured nanomaterial hazard screening as a first-tier approach. Critical parts of such assays are positive and negative controls that serve as measurement benchmarks. We present the cellular viability and corresponding particle characterization obtained with eight different cell lines that were exposed to Au-PEI and Au-PEG-COOH nanoparticles. We showed that polyethyleneimine- and carboxylate-polyethylene-glycol-conjugated gold nanoparticles (AuPEI and Au-PEG-COOH) qualified for positive and negative controls in the in vitro cell viability assays used for MNM toxicological screening.https://www.mdpi.com/2079-4991/15/2/79gold nanoparticlescell viabilityassay controlsMTSpolyethylene iminepolyethylene glycol |
| spellingShingle | Vytas Reipa Vincent A. Hackley Alessandro Tona Min Beom Heo Ye Ryeong Lee Tae Geol Lee Aaron Johnston-Peck Tae Joon Cho Well-Characterized Polyethyleneimine-/Carboxylated-Polyethylene-Glycol-Functionalized Gold Nanoparticles as Prospective Nanoscale Control Materials for In Vitro Cell Viability Assays: Particle Characterization and Toxicity Tests in Eight Mammalian Cell Lines Nanomaterials gold nanoparticles cell viability assay controls MTS polyethylene imine polyethylene glycol |
| title | Well-Characterized Polyethyleneimine-/Carboxylated-Polyethylene-Glycol-Functionalized Gold Nanoparticles as Prospective Nanoscale Control Materials for In Vitro Cell Viability Assays: Particle Characterization and Toxicity Tests in Eight Mammalian Cell Lines |
| title_full | Well-Characterized Polyethyleneimine-/Carboxylated-Polyethylene-Glycol-Functionalized Gold Nanoparticles as Prospective Nanoscale Control Materials for In Vitro Cell Viability Assays: Particle Characterization and Toxicity Tests in Eight Mammalian Cell Lines |
| title_fullStr | Well-Characterized Polyethyleneimine-/Carboxylated-Polyethylene-Glycol-Functionalized Gold Nanoparticles as Prospective Nanoscale Control Materials for In Vitro Cell Viability Assays: Particle Characterization and Toxicity Tests in Eight Mammalian Cell Lines |
| title_full_unstemmed | Well-Characterized Polyethyleneimine-/Carboxylated-Polyethylene-Glycol-Functionalized Gold Nanoparticles as Prospective Nanoscale Control Materials for In Vitro Cell Viability Assays: Particle Characterization and Toxicity Tests in Eight Mammalian Cell Lines |
| title_short | Well-Characterized Polyethyleneimine-/Carboxylated-Polyethylene-Glycol-Functionalized Gold Nanoparticles as Prospective Nanoscale Control Materials for In Vitro Cell Viability Assays: Particle Characterization and Toxicity Tests in Eight Mammalian Cell Lines |
| title_sort | well characterized polyethyleneimine carboxylated polyethylene glycol functionalized gold nanoparticles as prospective nanoscale control materials for in vitro cell viability assays particle characterization and toxicity tests in eight mammalian cell lines |
| topic | gold nanoparticles cell viability assay controls MTS polyethylene imine polyethylene glycol |
| url | https://www.mdpi.com/2079-4991/15/2/79 |
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