Increased Depth of Cellular Imaging in the Intact Lung Using Far-Red and Near-Infrared Fluorescent Probes
Scattering of shorter-wavelength visible light limits the fluorescence imaging depth of thick specimens such as whole organs. In this study, we report the use of four newly synthesized near-infrared and far-red fluorescence probes (excitation/emission, in nm: 644/670; 683/707; 786/814; 824/834) to i...
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| Format: | Article |
| Language: | English |
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Wiley
2006-01-01
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| Series: | International Journal of Biomedical Imaging |
| Online Access: | http://dx.doi.org/10.1155/IJBI/2006/37470 |
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| author | Abu-Bakr Al-Mehdi Mita Patel Abu Haroon Darla Reed B. Ohlsson-Wilhelm K. Muirhead Brian D. Gray |
| author_facet | Abu-Bakr Al-Mehdi Mita Patel Abu Haroon Darla Reed B. Ohlsson-Wilhelm K. Muirhead Brian D. Gray |
| author_sort | Abu-Bakr Al-Mehdi |
| collection | DOAJ |
| description | Scattering of shorter-wavelength visible light limits the
fluorescence imaging depth of thick specimens such as whole
organs. In this study, we report the use of four newly synthesized
near-infrared and far-red fluorescence probes
(excitation/emission, in nm: 644/670; 683/707; 786/814; 824/834)
to image tumor cells in the subpleural vasculature of the intact
rat lungs. Transpelural imaging of tumor cells labeled with
long-wavelength probes and expressing green fluorescent protein
(GFP; excitation/emission 488/507 nm) was done in the intact rat
lung after perfusate administration or intravenous injection. Our
results show that the average optimum imaging depth for the
long-wavelength probes is higher (27.8±0.7 μm) than for GFP (20±0.5 μm; p=0.008; n=50), corresponding to a
40% increase in the volume of tissue accessible for
high-resolution imaging. The maximum depth of cell visualization
was significantly improved with the novel dyes (36.4±1 μm from the pleural surface) compared with GFP (30.1±0.5 μm; p=0.01; n=50). Stable binding of the long-wavelength
vital dyes to the plasma membrane also permitted in vivo tracking
of injected tumor cells in the pulmonary vasculature. These probes
offer a significant improvement in the imaging quality of in situ
biological processes in the deeper regions of intact lungs. |
| format | Article |
| id | doaj-art-7d1f710c69c44a19838eef24e04b6935 |
| institution | Kabale University |
| issn | 1687-4188 1687-4196 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomedical Imaging |
| spelling | doaj-art-7d1f710c69c44a19838eef24e04b69352025-02-03T06:42:14ZengWileyInternational Journal of Biomedical Imaging1687-41881687-41962006-01-01200610.1155/IJBI/2006/3747037470Increased Depth of Cellular Imaging in the Intact Lung Using Far-Red and Near-Infrared Fluorescent ProbesAbu-Bakr Al-Mehdi0Mita Patel1Abu Haroon2Darla Reed3B. Ohlsson-Wilhelm4K. Muirhead5Brian D. Gray6Center for Lung Biology and Department of Pharmacology, College of Medicine, University of South Alabama, 307 N. University Boulevand, MSB 3370, Mobile, AL 36688, USACenter for Lung Biology and Department of Pharmacology, College of Medicine, University of South Alabama, 307 N. University Boulevand, MSB 3370, Mobile, AL 36688, USACenter for Lung Biology and Department of Pharmacology, College of Medicine, University of South Alabama, 307 N. University Boulevand, MSB 3370, Mobile, AL 36688, USACenter for Lung Biology and Department of Pharmacology, College of Medicine, University of South Alabama, 307 N. University Boulevand, MSB 3370, Mobile, AL 36688, USASciGro, Inc., Malvern, PA 19355, USASciGro, Inc., Malvern, PA 19355, USAPTI Research, Inc., Exton, PA 19341, USAScattering of shorter-wavelength visible light limits the fluorescence imaging depth of thick specimens such as whole organs. In this study, we report the use of four newly synthesized near-infrared and far-red fluorescence probes (excitation/emission, in nm: 644/670; 683/707; 786/814; 824/834) to image tumor cells in the subpleural vasculature of the intact rat lungs. Transpelural imaging of tumor cells labeled with long-wavelength probes and expressing green fluorescent protein (GFP; excitation/emission 488/507 nm) was done in the intact rat lung after perfusate administration or intravenous injection. Our results show that the average optimum imaging depth for the long-wavelength probes is higher (27.8±0.7 μm) than for GFP (20±0.5 μm; p=0.008; n=50), corresponding to a 40% increase in the volume of tissue accessible for high-resolution imaging. The maximum depth of cell visualization was significantly improved with the novel dyes (36.4±1 μm from the pleural surface) compared with GFP (30.1±0.5 μm; p=0.01; n=50). Stable binding of the long-wavelength vital dyes to the plasma membrane also permitted in vivo tracking of injected tumor cells in the pulmonary vasculature. These probes offer a significant improvement in the imaging quality of in situ biological processes in the deeper regions of intact lungs.http://dx.doi.org/10.1155/IJBI/2006/37470 |
| spellingShingle | Abu-Bakr Al-Mehdi Mita Patel Abu Haroon Darla Reed B. Ohlsson-Wilhelm K. Muirhead Brian D. Gray Increased Depth of Cellular Imaging in the Intact Lung Using Far-Red and Near-Infrared Fluorescent Probes International Journal of Biomedical Imaging |
| title | Increased Depth of Cellular Imaging in the Intact Lung Using Far-Red and Near-Infrared Fluorescent Probes |
| title_full | Increased Depth of Cellular Imaging in the Intact Lung Using Far-Red and Near-Infrared Fluorescent Probes |
| title_fullStr | Increased Depth of Cellular Imaging in the Intact Lung Using Far-Red and Near-Infrared Fluorescent Probes |
| title_full_unstemmed | Increased Depth of Cellular Imaging in the Intact Lung Using Far-Red and Near-Infrared Fluorescent Probes |
| title_short | Increased Depth of Cellular Imaging in the Intact Lung Using Far-Red and Near-Infrared Fluorescent Probes |
| title_sort | increased depth of cellular imaging in the intact lung using far red and near infrared fluorescent probes |
| url | http://dx.doi.org/10.1155/IJBI/2006/37470 |
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