Therapeutic potential of the probiotic Lactiplantibacillus plantarum BX 62 and its postbiotics in alleviating rheumatoid arthritis in mice

This study comprehensively investigated the probiotic potential of Lactiplantibacillus plantarum and its derived postbiotics in alleviating rheumatoid arthritis (RA) symptoms in mice. In vitro analyses established the probiotic characteristics of L. plantarum, highlighting its acid tolerance, bile s...

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Main Authors: Qi Qin, Gang Hu, Xiaoni Zhou, Ruizhe Zhu, Jianying Chen, Kangtai Zeng, Jingwu Wu, Jing Wei, Tingtao Chen, Xusheng Guo
Format: Article
Language:English
Published: Elsevier 2024-01-01
Series:Current Research in Food Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S2665927124002417
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author Qi Qin
Gang Hu
Xiaoni Zhou
Ruizhe Zhu
Jianying Chen
Kangtai Zeng
Jingwu Wu
Jing Wei
Tingtao Chen
Xusheng Guo
author_facet Qi Qin
Gang Hu
Xiaoni Zhou
Ruizhe Zhu
Jianying Chen
Kangtai Zeng
Jingwu Wu
Jing Wei
Tingtao Chen
Xusheng Guo
author_sort Qi Qin
collection DOAJ
description This study comprehensively investigated the probiotic potential of Lactiplantibacillus plantarum and its derived postbiotics in alleviating rheumatoid arthritis (RA) symptoms in mice. In vitro analyses established the probiotic characteristics of L. plantarum, highlighting its acid tolerance, bile salt resistance, and adhesion abilities. Subsequent in vivo experiments demonstrated that administering live L. plantarum significantly reduced arthritis scores, paw oedema, and intestinal permeability in RA mice, indicating its therapeutic potential. Furthermore, the study explored the effects of L. plantarum-derived postbiotics, including both the cellular remnants and supernatant, on RA. Notably, both the inactivated body of L. plantarum and its supernatant effectively reduced osteoarthritic damage, intestinal pathological changes, autoantibody levels, cytokine imbalances, and oxidative stress in RA mice. Additionally, qPCR analysis revealed that L. plantarum interventions restored gut microbiota composition towards a healthier profile, resembling that of normal mice. Collectively, these findings underscore the therapeutic benefits of L. plantarum and its postbiotics in ameliorating RA symptoms, emphasising their potential as novel therapeutic agents for managing RA.
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publisher Elsevier
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spelling doaj-art-7d1c7335de524d3980b839c31a2d74f12025-08-20T02:38:06ZengElsevierCurrent Research in Food Science2665-92712024-01-01910091510.1016/j.crfs.2024.100915Therapeutic potential of the probiotic Lactiplantibacillus plantarum BX 62 and its postbiotics in alleviating rheumatoid arthritis in miceQi Qin0Gang Hu1Xiaoni Zhou2Ruizhe Zhu3Jianying Chen4Kangtai Zeng5Jingwu Wu6Jing Wei7Tingtao Chen8Xusheng Guo9School of Life Sciences, Lanzhou University, Lanzhou, 730000, China; Probiotics and Life Health Institute, Lanzhou University, Lanzhou, 730000, ChinaSchool of Life Sciences, Nanchang University, Nanchang, 330031, China; National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Jiangxi Medical College, Nanchang University, Nanchang, 330031, Jiangxi, ChinaDepartment of Obstetrics & Gynecology, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330006, Jiangxi, ChinaNational Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Jiangxi Medical College, Nanchang University, Nanchang, 330031, Jiangxi, ChinaNational Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Jiangxi Medical College, Nanchang University, Nanchang, 330031, Jiangxi, ChinaSchool of Public Health, Jiangxi Medical College, Nanchang University, Nanchang, 330006, Jiangxi, ChinaSecond Clinical Medical College, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, ChinaNational Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Jiangxi Medical College, Nanchang University, Nanchang, 330031, Jiangxi, ChinaSchool of Life Sciences, Nanchang University, Nanchang, 330031, China; National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Jiangxi Medical College, Nanchang University, Nanchang, 330031, Jiangxi, China; School of Pharmacy, Jiangxi Medical College, Nanchang University, Nanchang, 330036, Jiangxi, China; Corresponding author. School of Life Sciences, Nanchang University, Nanchang, 330031, China.School of Life Sciences, Lanzhou University, Lanzhou, 730000, China; Probiotics and Life Health Institute, Lanzhou University, Lanzhou, 730000, China; Corresponding author. School of Life Sciences, Lanzhou University, Lanzhou, 730000, China.This study comprehensively investigated the probiotic potential of Lactiplantibacillus plantarum and its derived postbiotics in alleviating rheumatoid arthritis (RA) symptoms in mice. In vitro analyses established the probiotic characteristics of L. plantarum, highlighting its acid tolerance, bile salt resistance, and adhesion abilities. Subsequent in vivo experiments demonstrated that administering live L. plantarum significantly reduced arthritis scores, paw oedema, and intestinal permeability in RA mice, indicating its therapeutic potential. Furthermore, the study explored the effects of L. plantarum-derived postbiotics, including both the cellular remnants and supernatant, on RA. Notably, both the inactivated body of L. plantarum and its supernatant effectively reduced osteoarthritic damage, intestinal pathological changes, autoantibody levels, cytokine imbalances, and oxidative stress in RA mice. Additionally, qPCR analysis revealed that L. plantarum interventions restored gut microbiota composition towards a healthier profile, resembling that of normal mice. Collectively, these findings underscore the therapeutic benefits of L. plantarum and its postbiotics in ameliorating RA symptoms, emphasising their potential as novel therapeutic agents for managing RA.http://www.sciencedirect.com/science/article/pii/S2665927124002417Rheumatoid arthritisLactiplantibacillus plantarumOsteoarthritisPostbiotic
spellingShingle Qi Qin
Gang Hu
Xiaoni Zhou
Ruizhe Zhu
Jianying Chen
Kangtai Zeng
Jingwu Wu
Jing Wei
Tingtao Chen
Xusheng Guo
Therapeutic potential of the probiotic Lactiplantibacillus plantarum BX 62 and its postbiotics in alleviating rheumatoid arthritis in mice
Current Research in Food Science
Rheumatoid arthritis
Lactiplantibacillus plantarum
Osteoarthritis
Postbiotic
title Therapeutic potential of the probiotic Lactiplantibacillus plantarum BX 62 and its postbiotics in alleviating rheumatoid arthritis in mice
title_full Therapeutic potential of the probiotic Lactiplantibacillus plantarum BX 62 and its postbiotics in alleviating rheumatoid arthritis in mice
title_fullStr Therapeutic potential of the probiotic Lactiplantibacillus plantarum BX 62 and its postbiotics in alleviating rheumatoid arthritis in mice
title_full_unstemmed Therapeutic potential of the probiotic Lactiplantibacillus plantarum BX 62 and its postbiotics in alleviating rheumatoid arthritis in mice
title_short Therapeutic potential of the probiotic Lactiplantibacillus plantarum BX 62 and its postbiotics in alleviating rheumatoid arthritis in mice
title_sort therapeutic potential of the probiotic lactiplantibacillus plantarum bx 62 and its postbiotics in alleviating rheumatoid arthritis in mice
topic Rheumatoid arthritis
Lactiplantibacillus plantarum
Osteoarthritis
Postbiotic
url http://www.sciencedirect.com/science/article/pii/S2665927124002417
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