Metabolomics profiles revealed potential biomarkers of Pacific white shrimp (Litopenaeus vannamei) under cold and low salinity stress
Temperature and salinity are the most important environmental factors in the culture of Pacific white shrimp (Litopenaeus vannamei). This study aimed to investigate the effects of long-term low temperature and low salinity stress on the hepatopancreas metabolome of L. vannamei. A two-factor experime...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425000195 |
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| author | Zhenzhu Sun Pei Lin Haobin Mai Leling Chen Zonglu Wei Beiping Tan Chaoxia Ye |
| author_facet | Zhenzhu Sun Pei Lin Haobin Mai Leling Chen Zonglu Wei Beiping Tan Chaoxia Ye |
| author_sort | Zhenzhu Sun |
| collection | DOAJ |
| description | Temperature and salinity are the most important environmental factors in the culture of Pacific white shrimp (Litopenaeus vannamei). This study aimed to investigate the effects of long-term low temperature and low salinity stress on the hepatopancreas metabolome of L. vannamei. A two-factor experiment of temperature and salinity was designed with four treatments, namely HSHT (20psu, 28 °C), HSLT (20psu, 20 °C), LSHT (5psu, 28 °C), and LSLT (5psu, 20 °C). Results showed that weight gain ratio was significantly inhibited by low temperature but less affected by low salinity. LC-MS analysis revealed overlapping significant pathways, including ABC transporters, protein digestion, aminoacyl-tRNA biosynthesis, purine metabolism, and pyrimidine metabolism, with an additional pathway (mineral absorption) in the salinity change group. Moreover, seven metabolites were significantly involved during cold or salinity stress. Notably, dihydrothymine and stachyose may serve as biomarkers for measuring cold stress, while phosphorylcholine may indicate salinity stress levels. This study highlights the metabolic regulation of L. vannamei in response to low temperature and salinity, offering valuable insights into their domestication under cold and low-salt conditions. |
| format | Article |
| id | doaj-art-7d0ba63702fc4e0da7843795fa6ea627 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-7d0ba63702fc4e0da7843795fa6ea6272025-02-06T05:12:22ZengElsevierAquaculture Reports2352-51342025-03-0140102633Metabolomics profiles revealed potential biomarkers of Pacific white shrimp (Litopenaeus vannamei) under cold and low salinity stressZhenzhu Sun0Pei Lin1Haobin Mai2Leling Chen3Zonglu Wei4Beiping Tan5Chaoxia Ye6Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Institute of Modern Aquaculture Science and Engineering, School of Life Science, South China Normal University, Guangzhou 510631, China; School of Biology and Agriculture, Shaoguan University, Shaoguan 512005, ChinaGuangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Institute of Modern Aquaculture Science and Engineering, School of Life Science, South China Normal University, Guangzhou 510631, ChinaGuangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Institute of Modern Aquaculture Science and Engineering, School of Life Science, South China Normal University, Guangzhou 510631, ChinaGuangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Institute of Modern Aquaculture Science and Engineering, School of Life Science, South China Normal University, Guangzhou 510631, ChinaGuangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Institute of Modern Aquaculture Science and Engineering, School of Life Science, South China Normal University, Guangzhou 510631, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Institute of Modern Aquaculture Science and Engineering, School of Life Science, South China Normal University, Guangzhou 510631, China; Corresponding author.Temperature and salinity are the most important environmental factors in the culture of Pacific white shrimp (Litopenaeus vannamei). This study aimed to investigate the effects of long-term low temperature and low salinity stress on the hepatopancreas metabolome of L. vannamei. A two-factor experiment of temperature and salinity was designed with four treatments, namely HSHT (20psu, 28 °C), HSLT (20psu, 20 °C), LSHT (5psu, 28 °C), and LSLT (5psu, 20 °C). Results showed that weight gain ratio was significantly inhibited by low temperature but less affected by low salinity. LC-MS analysis revealed overlapping significant pathways, including ABC transporters, protein digestion, aminoacyl-tRNA biosynthesis, purine metabolism, and pyrimidine metabolism, with an additional pathway (mineral absorption) in the salinity change group. Moreover, seven metabolites were significantly involved during cold or salinity stress. Notably, dihydrothymine and stachyose may serve as biomarkers for measuring cold stress, while phosphorylcholine may indicate salinity stress levels. This study highlights the metabolic regulation of L. vannamei in response to low temperature and salinity, offering valuable insights into their domestication under cold and low-salt conditions.http://www.sciencedirect.com/science/article/pii/S2352513425000195Litopenaeus vannameiTemperatureSalinityStressMetabolomics |
| spellingShingle | Zhenzhu Sun Pei Lin Haobin Mai Leling Chen Zonglu Wei Beiping Tan Chaoxia Ye Metabolomics profiles revealed potential biomarkers of Pacific white shrimp (Litopenaeus vannamei) under cold and low salinity stress Aquaculture Reports Litopenaeus vannamei Temperature Salinity Stress Metabolomics |
| title | Metabolomics profiles revealed potential biomarkers of Pacific white shrimp (Litopenaeus vannamei) under cold and low salinity stress |
| title_full | Metabolomics profiles revealed potential biomarkers of Pacific white shrimp (Litopenaeus vannamei) under cold and low salinity stress |
| title_fullStr | Metabolomics profiles revealed potential biomarkers of Pacific white shrimp (Litopenaeus vannamei) under cold and low salinity stress |
| title_full_unstemmed | Metabolomics profiles revealed potential biomarkers of Pacific white shrimp (Litopenaeus vannamei) under cold and low salinity stress |
| title_short | Metabolomics profiles revealed potential biomarkers of Pacific white shrimp (Litopenaeus vannamei) under cold and low salinity stress |
| title_sort | metabolomics profiles revealed potential biomarkers of pacific white shrimp litopenaeus vannamei under cold and low salinity stress |
| topic | Litopenaeus vannamei Temperature Salinity Stress Metabolomics |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425000195 |
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