A green approach to the analysis of co-administered ampicillin/sulbactam and paracetamol in human urine
The novelty of this work is the simultaneous analysis of sulbactam (SUL), ampicillin (AMP), and paracetamol (PARA) in human urine samples, using the environmentally benign RP-HPLC method. A C18 column was used in chromatographic separation using potassium dihydrogen phosphate (10 mmol L–1, pH 5)/eth...
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| Format: | Article |
| Language: | English |
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2022-06-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2022-0015 |
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| author | SELIM BASMA M. ABDELSALAM RANDA A. EL-GINDY ALAA EID BASMA G. NEAMATALLAH THIKRYAT KHAYYAT AHDAB N. HADAD GHADA M. ELKHOUDARY MAHMOUD M. |
| author_facet | SELIM BASMA M. ABDELSALAM RANDA A. EL-GINDY ALAA EID BASMA G. NEAMATALLAH THIKRYAT KHAYYAT AHDAB N. HADAD GHADA M. ELKHOUDARY MAHMOUD M. |
| author_sort | SELIM BASMA M. |
| collection | DOAJ |
| description | The novelty of this work is the simultaneous analysis of sulbactam (SUL), ampicillin (AMP), and paracetamol (PARA) in human urine samples, using the environmentally benign RP-HPLC method. A C18 column was used in chromatographic separation using potassium dihydrogen phosphate (10 mmol L–1, pH 5)/ethanol (90 %, V/V) as the mobile phase; flow rate was 1.00 mL min–1. UV detection at 220 nm was used for quantification. The proposed method showed good linearity in the concentration ranges of 2.20–250.00 μg mL–1 for SUL, 2.50–250.00 μg mL–1 for PARA, and 14.50–250.00 μg mL–1 for AMP. Direct injection of urine samples with no prior extraction was performed. This method was found successful in moving towards greener studies of drugs’ urinary excretion, by decreasing hazardous solvent consumption and waste. Moreover, the method was applied to investigate the urinary excretion of the drugs and possible interaction between ampicillin and paracetamol. |
| format | Article |
| id | doaj-art-7cdee1cb5e084fc7aab6985cd5bff6fb |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2022-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-7cdee1cb5e084fc7aab6985cd5bff6fb2025-02-02T15:12:33ZengSciendoActa Pharmaceutica1846-95582022-06-0172225927410.2478/acph-2022-0015A green approach to the analysis of co-administered ampicillin/sulbactam and paracetamol in human urineSELIM BASMA M.0ABDELSALAM RANDA A.1EL-GINDY ALAA2EID BASMA G.3NEAMATALLAH THIKRYAT4KHAYYAT AHDAB N.5HADAD GHADA M.6ELKHOUDARY MAHMOUD M.71Pharmaceutical Chemistry Department Faculty of PharmacySinai University, North Sinai, Al’Arish, Egypt2Pharmaceutical Analytical Chemistry Department, Faculty of PharmacySuez Canal University, Ismailia, Egypt2Pharmaceutical Analytical Chemistry Department, Faculty of PharmacySuez Canal University, Ismailia, Egypt3Department of Pharmacology & Toxicology Faculty of PharmacyKing Abdulaziz University, ShaqraSaudi Arabia3Department of Pharmacology & Toxicology Faculty of PharmacyKing Abdulaziz University, ShaqraSaudi Arabia4Department of Pharmaceutical Chemistry Faculty of PharmacyKing Abdulaziz University, Jeddah, Saudi Arabia2Pharmaceutical Analytical Chemistry Department, Faculty of PharmacySuez Canal University, Ismailia, Egypt5Pharmaceutical Chemistry Department Faculty of PharmaHorus University-Egypt, New Damietta, EgyptThe novelty of this work is the simultaneous analysis of sulbactam (SUL), ampicillin (AMP), and paracetamol (PARA) in human urine samples, using the environmentally benign RP-HPLC method. A C18 column was used in chromatographic separation using potassium dihydrogen phosphate (10 mmol L–1, pH 5)/ethanol (90 %, V/V) as the mobile phase; flow rate was 1.00 mL min–1. UV detection at 220 nm was used for quantification. The proposed method showed good linearity in the concentration ranges of 2.20–250.00 μg mL–1 for SUL, 2.50–250.00 μg mL–1 for PARA, and 14.50–250.00 μg mL–1 for AMP. Direct injection of urine samples with no prior extraction was performed. This method was found successful in moving towards greener studies of drugs’ urinary excretion, by decreasing hazardous solvent consumption and waste. Moreover, the method was applied to investigate the urinary excretion of the drugs and possible interaction between ampicillin and paracetamol.https://doi.org/10.2478/acph-2022-0015ampicillinparacetamolsulbactamhplcgreen analytical chemistrydrug-drug interactionurinary excretion |
| spellingShingle | SELIM BASMA M. ABDELSALAM RANDA A. EL-GINDY ALAA EID BASMA G. NEAMATALLAH THIKRYAT KHAYYAT AHDAB N. HADAD GHADA M. ELKHOUDARY MAHMOUD M. A green approach to the analysis of co-administered ampicillin/sulbactam and paracetamol in human urine Acta Pharmaceutica ampicillin paracetamol sulbactam hplc green analytical chemistry drug-drug interaction urinary excretion |
| title | A green approach to the analysis of co-administered ampicillin/sulbactam and paracetamol in human urine |
| title_full | A green approach to the analysis of co-administered ampicillin/sulbactam and paracetamol in human urine |
| title_fullStr | A green approach to the analysis of co-administered ampicillin/sulbactam and paracetamol in human urine |
| title_full_unstemmed | A green approach to the analysis of co-administered ampicillin/sulbactam and paracetamol in human urine |
| title_short | A green approach to the analysis of co-administered ampicillin/sulbactam and paracetamol in human urine |
| title_sort | green approach to the analysis of co administered ampicillin sulbactam and paracetamol in human urine |
| topic | ampicillin paracetamol sulbactam hplc green analytical chemistry drug-drug interaction urinary excretion |
| url | https://doi.org/10.2478/acph-2022-0015 |
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