Highly-sensitive electrochemiluminescence biosensor for detection of inosine monophosphate in meat based on graphdiyne/AuNPs/luminol nanocomposites
Inosine monophosphate (IMP), as a critical umami substance, is one of the most important indicators for evaluating the quality of meat products. Here, a sensitive electrochemiluminescence (ECL) biosensor based on graphdiyne (GDY)/AuNPs/luminol nanocomposites was constructed to detect IMP. The GDY/Au...
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Tsinghua University Press
2023-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002592 |
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| author | Jing Liu Yizhong Shen Guangxian Wang Yaodong Xiang Yemin Guo Xia Sun Yuan Liu |
| author_facet | Jing Liu Yizhong Shen Guangxian Wang Yaodong Xiang Yemin Guo Xia Sun Yuan Liu |
| author_sort | Jing Liu |
| collection | DOAJ |
| description | Inosine monophosphate (IMP), as a critical umami substance, is one of the most important indicators for evaluating the quality of meat products. Here, a sensitive electrochemiluminescence (ECL) biosensor based on graphdiyne (GDY)/AuNPs/luminol nanocomposites was constructed to detect IMP. The GDY/AuNPs/luminol nanocomposites were synthesized by using simple one-pot method. GDY utilized its 2D framework to disperse and fix gold nanoparticles, which inhibited the agglomeration of gold nanoparticles and greatly improved its stability and catalytic properties. Importantly, GDY/AuNPs/luminol nanocomposites showed excellent catalytic ability and superior ECL activity towards luminol-H2O2 systems due to the synergistic effect of GDY and AuNPs. Under optimal conditions, the prepared biosensor exhibited a wide linear range from 0.01 g/L to 20 g/L, a satisfactory limit detection of 0.0013 g/L, as well as an excellent specificity. Moreover, we carried out the precise analysis of IMP in actual meat samples with acceptable results compared to the liquid chromatography. We believe that this work could offer an efficient ECL platform for accurate and reliable report of IMP levels, which is significant for maintaining food quality and safety. |
| format | Article |
| id | doaj-art-7cc7a4540ebf40bbb7146490ed678187 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-7cc7a4540ebf40bbb7146490ed6781872025-02-03T07:06:25ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112411491156Highly-sensitive electrochemiluminescence biosensor for detection of inosine monophosphate in meat based on graphdiyne/AuNPs/luminol nanocompositesJing Liu0Yizhong Shen1Guangxian Wang2Yaodong Xiang3Yemin Guo4Xia Sun5Yuan Liu6School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, ChinaSchool of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, ChinaSchool of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, ChinaSchool of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, China; Corresponding authors.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Corresponding authors.Inosine monophosphate (IMP), as a critical umami substance, is one of the most important indicators for evaluating the quality of meat products. Here, a sensitive electrochemiluminescence (ECL) biosensor based on graphdiyne (GDY)/AuNPs/luminol nanocomposites was constructed to detect IMP. The GDY/AuNPs/luminol nanocomposites were synthesized by using simple one-pot method. GDY utilized its 2D framework to disperse and fix gold nanoparticles, which inhibited the agglomeration of gold nanoparticles and greatly improved its stability and catalytic properties. Importantly, GDY/AuNPs/luminol nanocomposites showed excellent catalytic ability and superior ECL activity towards luminol-H2O2 systems due to the synergistic effect of GDY and AuNPs. Under optimal conditions, the prepared biosensor exhibited a wide linear range from 0.01 g/L to 20 g/L, a satisfactory limit detection of 0.0013 g/L, as well as an excellent specificity. Moreover, we carried out the precise analysis of IMP in actual meat samples with acceptable results compared to the liquid chromatography. We believe that this work could offer an efficient ECL platform for accurate and reliable report of IMP levels, which is significant for maintaining food quality and safety.http://www.sciencedirect.com/science/article/pii/S2213453022002592Inosine monophosphate (IMP)ElectrochemiluminescenceGraphdiyne (GDY)/AuNPs/luminolBienzyme |
| spellingShingle | Jing Liu Yizhong Shen Guangxian Wang Yaodong Xiang Yemin Guo Xia Sun Yuan Liu Highly-sensitive electrochemiluminescence biosensor for detection of inosine monophosphate in meat based on graphdiyne/AuNPs/luminol nanocomposites Food Science and Human Wellness Inosine monophosphate (IMP) Electrochemiluminescence Graphdiyne (GDY)/AuNPs/luminol Bienzyme |
| title | Highly-sensitive electrochemiluminescence biosensor for detection of inosine monophosphate in meat based on graphdiyne/AuNPs/luminol nanocomposites |
| title_full | Highly-sensitive electrochemiluminescence biosensor for detection of inosine monophosphate in meat based on graphdiyne/AuNPs/luminol nanocomposites |
| title_fullStr | Highly-sensitive electrochemiluminescence biosensor for detection of inosine monophosphate in meat based on graphdiyne/AuNPs/luminol nanocomposites |
| title_full_unstemmed | Highly-sensitive electrochemiluminescence biosensor for detection of inosine monophosphate in meat based on graphdiyne/AuNPs/luminol nanocomposites |
| title_short | Highly-sensitive electrochemiluminescence biosensor for detection of inosine monophosphate in meat based on graphdiyne/AuNPs/luminol nanocomposites |
| title_sort | highly sensitive electrochemiluminescence biosensor for detection of inosine monophosphate in meat based on graphdiyne aunps luminol nanocomposites |
| topic | Inosine monophosphate (IMP) Electrochemiluminescence Graphdiyne (GDY)/AuNPs/luminol Bienzyme |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002592 |
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