Introduction of Panax notoginseng into pine forests significantly enhances the diversity, stochastic processes, and network complexity of nitrogen-fixing bacteria in the soil
IntroductionNitrogen-fixing bacteria (NFB) have a pivotal impact on the nitrogen cycle within agroforestry systems. The organic management of the Panax notoginseng (sanqi)-Pinus armandii agroforestry (SPA) system resulted in nitrogen deficiency because of the lack of application of chemical fertiliz...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-02-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1531875/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548271840559104 |
|---|---|
| author | Xiaoyan Zhao Shu He Rui Rui Jingying Hei Xiahong He Shu Wang |
| author_facet | Xiaoyan Zhao Shu He Rui Rui Jingying Hei Xiahong He Shu Wang |
| author_sort | Xiaoyan Zhao |
| collection | DOAJ |
| description | IntroductionNitrogen-fixing bacteria (NFB) have a pivotal impact on the nitrogen cycle within agroforestry systems. The organic management of the Panax notoginseng (sanqi)-Pinus armandii agroforestry (SPA) system resulted in nitrogen deficiency because of the lack of application of chemical fertilizers. Therefore, assessing the variability in NFB due to the cultivation of sanqi in the SPA system becomes crucial.MethodsThe seasonal dynamics in the abundance, diversity, and community structure of NFB in the soil of monocropping pine (MP) and SPA systems were assessed using real-time quantitative polymerase chain reaction and high-throughput sequencing technology.Results and discussionSanqi cultivation triggered a decrease in the abundance of NFB but increased α diversity. Additionally, significant differences in the community structure of NFB were noted between the MP and SPA systems. Moreover, the abundance of Bradyrhizobium and Azospirillum increased in the soil after sanqi was cultivated. Furthermore, the cultivation of sanqi broadened the ecological niche breadth of NFB and increased the stochasticity in its community structure assembly (i.e., dispersal limitation). Additionally, the SPA system increased the network complexity but not the stability of NFB. The structural equation model (SEM) revealed that pH directly impacted the network complexity and stability of NFB in the SPA system. Sanqi cultivation positively influences the community characteristics of NFB in the soil in the SPA system. Our study provides new insights into nitrogen cycling and utilization in the SPA system. |
| format | Article |
| id | doaj-art-7ca52f820a58478b9b30c63bab3b0bfb |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-7ca52f820a58478b9b30c63bab3b0bfb2025-02-03T06:33:43ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15318751531875Introduction of Panax notoginseng into pine forests significantly enhances the diversity, stochastic processes, and network complexity of nitrogen-fixing bacteria in the soilXiaoyan Zhao0Shu He1Rui Rui2Jingying Hei3Xiahong He4Shu Wang5Yunnan Provincial Key Laboratory for Conservation and Utilization of In-forest Resource, Southwest Forestry University, Kunming, ChinaYunnan Provincial Key Laboratory for Conservation and Utilization of In-forest Resource, Southwest Forestry University, Kunming, ChinaYunnan Provincial Key Laboratory for Conservation and Utilization of In-forest Resource, Southwest Forestry University, Kunming, ChinaYunnan Provincial Key Laboratory for Conservation and Utilization of In-forest Resource, Southwest Forestry University, Kunming, ChinaYunnan Provincial Key Laboratory for Conservation and Utilization of In-forest Resource, Southwest Forestry University, Kunming, ChinaSouthwest Research Center for Engineering Technology of Landscape Architecture (State Forestry and Grassland Administration), Kunming, Yunnan, ChinaIntroductionNitrogen-fixing bacteria (NFB) have a pivotal impact on the nitrogen cycle within agroforestry systems. The organic management of the Panax notoginseng (sanqi)-Pinus armandii agroforestry (SPA) system resulted in nitrogen deficiency because of the lack of application of chemical fertilizers. Therefore, assessing the variability in NFB due to the cultivation of sanqi in the SPA system becomes crucial.MethodsThe seasonal dynamics in the abundance, diversity, and community structure of NFB in the soil of monocropping pine (MP) and SPA systems were assessed using real-time quantitative polymerase chain reaction and high-throughput sequencing technology.Results and discussionSanqi cultivation triggered a decrease in the abundance of NFB but increased α diversity. Additionally, significant differences in the community structure of NFB were noted between the MP and SPA systems. Moreover, the abundance of Bradyrhizobium and Azospirillum increased in the soil after sanqi was cultivated. Furthermore, the cultivation of sanqi broadened the ecological niche breadth of NFB and increased the stochasticity in its community structure assembly (i.e., dispersal limitation). Additionally, the SPA system increased the network complexity but not the stability of NFB. The structural equation model (SEM) revealed that pH directly impacted the network complexity and stability of NFB in the SPA system. Sanqi cultivation positively influences the community characteristics of NFB in the soil in the SPA system. Our study provides new insights into nitrogen cycling and utilization in the SPA system.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1531875/fullPanax notoginsengnitrogen-fixing bacterianifH genehigh-throughput sequencingstructural equation model |
| spellingShingle | Xiaoyan Zhao Shu He Rui Rui Jingying Hei Xiahong He Shu Wang Introduction of Panax notoginseng into pine forests significantly enhances the diversity, stochastic processes, and network complexity of nitrogen-fixing bacteria in the soil Frontiers in Microbiology Panax notoginseng nitrogen-fixing bacteria nifH gene high-throughput sequencing structural equation model |
| title | Introduction of Panax notoginseng into pine forests significantly enhances the diversity, stochastic processes, and network complexity of nitrogen-fixing bacteria in the soil |
| title_full | Introduction of Panax notoginseng into pine forests significantly enhances the diversity, stochastic processes, and network complexity of nitrogen-fixing bacteria in the soil |
| title_fullStr | Introduction of Panax notoginseng into pine forests significantly enhances the diversity, stochastic processes, and network complexity of nitrogen-fixing bacteria in the soil |
| title_full_unstemmed | Introduction of Panax notoginseng into pine forests significantly enhances the diversity, stochastic processes, and network complexity of nitrogen-fixing bacteria in the soil |
| title_short | Introduction of Panax notoginseng into pine forests significantly enhances the diversity, stochastic processes, and network complexity of nitrogen-fixing bacteria in the soil |
| title_sort | introduction of panax notoginseng into pine forests significantly enhances the diversity stochastic processes and network complexity of nitrogen fixing bacteria in the soil |
| topic | Panax notoginseng nitrogen-fixing bacteria nifH gene high-throughput sequencing structural equation model |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1531875/full |
| work_keys_str_mv | AT xiaoyanzhao introductionofpanaxnotoginsengintopineforestssignificantlyenhancesthediversitystochasticprocessesandnetworkcomplexityofnitrogenfixingbacteriainthesoil AT shuhe introductionofpanaxnotoginsengintopineforestssignificantlyenhancesthediversitystochasticprocessesandnetworkcomplexityofnitrogenfixingbacteriainthesoil AT ruirui introductionofpanaxnotoginsengintopineforestssignificantlyenhancesthediversitystochasticprocessesandnetworkcomplexityofnitrogenfixingbacteriainthesoil AT jingyinghei introductionofpanaxnotoginsengintopineforestssignificantlyenhancesthediversitystochasticprocessesandnetworkcomplexityofnitrogenfixingbacteriainthesoil AT xiahonghe introductionofpanaxnotoginsengintopineforestssignificantlyenhancesthediversitystochasticprocessesandnetworkcomplexityofnitrogenfixingbacteriainthesoil AT shuwang introductionofpanaxnotoginsengintopineforestssignificantlyenhancesthediversitystochasticprocessesandnetworkcomplexityofnitrogenfixingbacteriainthesoil |