High Prevalence of blaOXA-2 and blaOXA-10 Genes in the β-Lactam-Resistant Clinical Isolates of Pseudomonas aeruginosa
Background: Increased resistance to β-lactams is one of the most important considerations about Pseudomonas aeruginosa. This study aimed to investigate the prevalence of blaOXA-2 and blaOXA-10 genes in β-lactam-resistant P. aeruginosa. Methods: In this study, 100 clinical isolates were collected, a...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Mazandaran University of Medical Sciences and Health Services
2022-05-01
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| Series: | Research in Molecular Medicine |
| Subjects: | |
| Online Access: | http://rmm.mazums.ac.ir/article-1-475-en.pdf |
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| Summary: | Background: Increased resistance to β-lactams is one of the most important considerations about Pseudomonas aeruginosa. This study aimed to investigate the prevalence of blaOXA-2 and blaOXA-10 genes in β-lactam-resistant P. aeruginosa.
Methods: In this study, 100 clinical isolates were collected, and β-lactam-resistant strains were identified using the disk agar diffusion test. Bacterial DNA was extracted by the sodium dodecyl sulfate method, and the polymerase chain reaction was used to identify blaOXA-2 and blaOXA-10 β-lactamase genes.
Results: In total, 43 isolates were resistant to at least one of the β-lactams tested. Piperacillin-tazobactam and aztreonam were the most and least effective antibiotics, respectively. The resistance rate to a panel of β-lactams ranged from 12% to 37%. The highest rate of antibiotic resistance was related to wound and catheter specimens in burn and intensive care units. A significant relationship was observed between β-lactams resistance and the presence of blaOXA-2 and blaOXA-10 genes. Among 43 resistant isolates, 100% and 83.72% carried the blaOXA-2 and blaOXA-10 genes, respectively.
Conclusion: High β-lactam resistance rates and prevalence of blaOXA-2 and blaOXA-10 in this study indicate these enzymes' significant role in resistance to β-lactams, possibly due to overuse of these antibiotics and their easier access. |
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| ISSN: | 2322-1348 2322-133X |