Mechanical Strains Induced in Osteoblasts by Use of Point Femtosecond Laser Targeting
A study demonstrating how ultrafast laser radiation stimulates osteoblasts is presented. The study employed a custom made optical system that allowed for simultaneous confocal cell imaging and targeted femtosecond pulse laser irradiation. When femtosecond laser light was focused onto a single cell,...
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| Format: | Article |
| Language: | English |
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Wiley
2006-01-01
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| Series: | International Journal of Biomedical Imaging |
| Online Access: | http://dx.doi.org/10.1155/IJBI/2006/21304 |
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| _version_ | 1832568440664096768 |
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| author | Charles Cranfield Ze'ev Bomzon Daniel Day Min Gu Sarah Cartmell |
| author_facet | Charles Cranfield Ze'ev Bomzon Daniel Day Min Gu Sarah Cartmell |
| author_sort | Charles Cranfield |
| collection | DOAJ |
| description | A study demonstrating how ultrafast laser radiation stimulates osteoblasts is presented. The study employed a custom made optical system that allowed for simultaneous confocal cell imaging and targeted femtosecond pulse laser irradiation. When femtosecond laser light was focused onto a single cell, a rise in intracellular Ca2+ levels was observed followed by contraction of the targeted cell. This contraction caused deformation of neighbouring cells leading to a heterogeneous strain field throughout the monolayer. Quantification of the strain fields in the monolayer using digital image correlation revealed local strains much higher than threshold values typically reported to stimulate extracellular bone matrix production in vitro. This use of point targeting with femtosecond pulse lasers could provide a new method for stimulating cell activity in orthopaedic tissue engineering. |
| format | Article |
| id | doaj-art-7c79da20a38e4b3b96558c0dcbcf30d6 |
| institution | Kabale University |
| issn | 1687-4188 1687-4196 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomedical Imaging |
| spelling | doaj-art-7c79da20a38e4b3b96558c0dcbcf30d62025-02-03T00:59:08ZengWileyInternational Journal of Biomedical Imaging1687-41881687-41962006-01-01200610.1155/IJBI/2006/2130421304Mechanical Strains Induced in Osteoblasts by Use of Point Femtosecond Laser TargetingCharles Cranfield0Ze'ev Bomzon1Daniel Day2Min Gu3Sarah Cartmell4Centre for Micro-Photonics, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, Hawthorn, VIC 3122, AustraliaCentre for Micro-Photonics, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, Hawthorn, VIC 3122, AustraliaCentre for Micro-Photonics, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, Hawthorn, VIC 3122, AustraliaCentre for Micro-Photonics, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, Hawthorn, VIC 3122, AustraliaInstitute for Science and Technology in Medicine, Keele University, Keele, Staffordshire ST4 7QB, UKA study demonstrating how ultrafast laser radiation stimulates osteoblasts is presented. The study employed a custom made optical system that allowed for simultaneous confocal cell imaging and targeted femtosecond pulse laser irradiation. When femtosecond laser light was focused onto a single cell, a rise in intracellular Ca2+ levels was observed followed by contraction of the targeted cell. This contraction caused deformation of neighbouring cells leading to a heterogeneous strain field throughout the monolayer. Quantification of the strain fields in the monolayer using digital image correlation revealed local strains much higher than threshold values typically reported to stimulate extracellular bone matrix production in vitro. This use of point targeting with femtosecond pulse lasers could provide a new method for stimulating cell activity in orthopaedic tissue engineering.http://dx.doi.org/10.1155/IJBI/2006/21304 |
| spellingShingle | Charles Cranfield Ze'ev Bomzon Daniel Day Min Gu Sarah Cartmell Mechanical Strains Induced in Osteoblasts by Use of Point Femtosecond Laser Targeting International Journal of Biomedical Imaging |
| title | Mechanical Strains Induced in Osteoblasts by Use of Point Femtosecond Laser Targeting |
| title_full | Mechanical Strains Induced in Osteoblasts by Use of Point Femtosecond Laser Targeting |
| title_fullStr | Mechanical Strains Induced in Osteoblasts by Use of Point Femtosecond Laser Targeting |
| title_full_unstemmed | Mechanical Strains Induced in Osteoblasts by Use of Point Femtosecond Laser Targeting |
| title_short | Mechanical Strains Induced in Osteoblasts by Use of Point Femtosecond Laser Targeting |
| title_sort | mechanical strains induced in osteoblasts by use of point femtosecond laser targeting |
| url | http://dx.doi.org/10.1155/IJBI/2006/21304 |
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