Alkaloid Profile, Anticholinesterase and Antioxidant Activities, and Sexual Propagation in <i>Hieronymiella peruviana</i> (Amaryllidaceae)
<i>Hieronymiella peruviana</i>, a recently described endemic species from southern Peru, belongs to the Amaryllidaceae family and is known for its diversity of alkaloids. Amaryllidoideae have been studied for their diverse biological activities, particularly for their properties in treat...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/2/281 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587650834366464 |
|---|---|
| author | Olimpia Llalla-Cordova Javier E. Ortiz Luciana R. Tallini Laura Torras-Claveria Jaume Bastida Lorena Celina Luna Gabriela E. Feresin |
| author_facet | Olimpia Llalla-Cordova Javier E. Ortiz Luciana R. Tallini Laura Torras-Claveria Jaume Bastida Lorena Celina Luna Gabriela E. Feresin |
| author_sort | Olimpia Llalla-Cordova |
| collection | DOAJ |
| description | <i>Hieronymiella peruviana</i>, a recently described endemic species from southern Peru, belongs to the Amaryllidaceae family and is known for its diversity of alkaloids. Amaryllidoideae have been studied for their diverse biological activities, particularly for their properties in treating neurodegenerative diseases. This work examines the alkaloidal profile using GC-MS and UPLC-MS/MS of alkaloid-enriched extracts obtained from the leaves and bulbs of <i>H. peruviana</i> and their inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) enzymes. In addition, the phenolic and flavonoid content in the methanolic extract from bulbs was quantified and the antioxidant capacity was evaluated. Moreover, the seed germination was investigated under four temperature conditions (15, 20, 25, and 30 °C). Twenty-two alkaloids, most of them of the homolycorine- and galanthamine-type, including galanthamine, were identified in the alkaloid extracts by means of GC-MS and UPLC-MS/MS analysis. Lycorine and 8-<i>O</i>-Demethylhomolycorine were the most abundant alkaloids in the bulbs and leaves, respectively. The leaves and bulbs alkaloid extracts demonstrated strong AChE inhibition (IC<sub>50</sub> = 5.20–8.60 µg/mL) and moderate BuChE inhibition (IC<sub>50</sub> = 90.20–122.76 µg/mL). The bulbs’ methanolic extract exhibited mild antioxidant activity, showing 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) values of 16.36% at 500 μg/mL and 58.31 mg Trolox equivalents (TE)/g, respectively. Seed germination was most effective at 20 °C and 25 °C. Finally, 60 days after germination, the survival rate of <i>H. peruviana</i> seedlings was 48.33%. These findings establish <i>H. peruviana</i> as a promising source of bioactive alkaloids with potential pharmacological and therapeutic applications, as well as providing critical insights for its propagation and conservation. |
| format | Article |
| id | doaj-art-7c72737730cf40b3bc19d093cfc09096 |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-7c72737730cf40b3bc19d093cfc090962025-01-24T13:47:05ZengMDPI AGPlants2223-77472025-01-0114228110.3390/plants14020281Alkaloid Profile, Anticholinesterase and Antioxidant Activities, and Sexual Propagation in <i>Hieronymiella peruviana</i> (Amaryllidaceae)Olimpia Llalla-Cordova0Javier E. Ortiz1Luciana R. Tallini2Laura Torras-Claveria3Jaume Bastida4Lorena Celina Luna5Gabriela E. Feresin6Instituto de Biotecnología, Facultad de Ingeniería, Universidad Nacional de San Juan, Av. Libertador General San Martín 1109 (O), San Juan CP5400, ArgentinaInstituto de Biotecnología, Facultad de Ingeniería, Universidad Nacional de San Juan, Av. Libertador General San Martín 1109 (O), San Juan CP5400, ArgentinaFaculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752, Porto Alegre 90610-000, RS, BrazilDepartament de Biologia, Sanitat i Medi Ambient, Facultat de Farmàcia i Ciències de l’Alimentació, Universitat de Barcelona, Av. Joan XXIII 27-31, 08028 Barcelona, SpainDepartament de Biologia, Sanitat i Medi Ambient, Facultat de Farmàcia i Ciències de l’Alimentació, Universitat de Barcelona, Av. Joan XXIII 27-31, 08028 Barcelona, SpainInstituto de Biotecnología, Facultad de Ingeniería, Universidad Nacional de San Juan, Av. Libertador General San Martín 1109 (O), San Juan CP5400, ArgentinaInstituto de Biotecnología, Facultad de Ingeniería, Universidad Nacional de San Juan, Av. Libertador General San Martín 1109 (O), San Juan CP5400, Argentina<i>Hieronymiella peruviana</i>, a recently described endemic species from southern Peru, belongs to the Amaryllidaceae family and is known for its diversity of alkaloids. Amaryllidoideae have been studied for their diverse biological activities, particularly for their properties in treating neurodegenerative diseases. This work examines the alkaloidal profile using GC-MS and UPLC-MS/MS of alkaloid-enriched extracts obtained from the leaves and bulbs of <i>H. peruviana</i> and their inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) enzymes. In addition, the phenolic and flavonoid content in the methanolic extract from bulbs was quantified and the antioxidant capacity was evaluated. Moreover, the seed germination was investigated under four temperature conditions (15, 20, 25, and 30 °C). Twenty-two alkaloids, most of them of the homolycorine- and galanthamine-type, including galanthamine, were identified in the alkaloid extracts by means of GC-MS and UPLC-MS/MS analysis. Lycorine and 8-<i>O</i>-Demethylhomolycorine were the most abundant alkaloids in the bulbs and leaves, respectively. The leaves and bulbs alkaloid extracts demonstrated strong AChE inhibition (IC<sub>50</sub> = 5.20–8.60 µg/mL) and moderate BuChE inhibition (IC<sub>50</sub> = 90.20–122.76 µg/mL). The bulbs’ methanolic extract exhibited mild antioxidant activity, showing 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) values of 16.36% at 500 μg/mL and 58.31 mg Trolox equivalents (TE)/g, respectively. Seed germination was most effective at 20 °C and 25 °C. Finally, 60 days after germination, the survival rate of <i>H. peruviana</i> seedlings was 48.33%. These findings establish <i>H. peruviana</i> as a promising source of bioactive alkaloids with potential pharmacological and therapeutic applications, as well as providing critical insights for its propagation and conservation.https://www.mdpi.com/2223-7747/14/2/281AmaryllidaceaeHieronymiellaanti-oxidantgerminationcholinesterasesPeru |
| spellingShingle | Olimpia Llalla-Cordova Javier E. Ortiz Luciana R. Tallini Laura Torras-Claveria Jaume Bastida Lorena Celina Luna Gabriela E. Feresin Alkaloid Profile, Anticholinesterase and Antioxidant Activities, and Sexual Propagation in <i>Hieronymiella peruviana</i> (Amaryllidaceae) Plants Amaryllidaceae Hieronymiella anti-oxidant germination cholinesterases Peru |
| title | Alkaloid Profile, Anticholinesterase and Antioxidant Activities, and Sexual Propagation in <i>Hieronymiella peruviana</i> (Amaryllidaceae) |
| title_full | Alkaloid Profile, Anticholinesterase and Antioxidant Activities, and Sexual Propagation in <i>Hieronymiella peruviana</i> (Amaryllidaceae) |
| title_fullStr | Alkaloid Profile, Anticholinesterase and Antioxidant Activities, and Sexual Propagation in <i>Hieronymiella peruviana</i> (Amaryllidaceae) |
| title_full_unstemmed | Alkaloid Profile, Anticholinesterase and Antioxidant Activities, and Sexual Propagation in <i>Hieronymiella peruviana</i> (Amaryllidaceae) |
| title_short | Alkaloid Profile, Anticholinesterase and Antioxidant Activities, and Sexual Propagation in <i>Hieronymiella peruviana</i> (Amaryllidaceae) |
| title_sort | alkaloid profile anticholinesterase and antioxidant activities and sexual propagation in i hieronymiella peruviana i amaryllidaceae |
| topic | Amaryllidaceae Hieronymiella anti-oxidant germination cholinesterases Peru |
| url | https://www.mdpi.com/2223-7747/14/2/281 |
| work_keys_str_mv | AT olimpiallallacordova alkaloidprofileanticholinesteraseandantioxidantactivitiesandsexualpropagationinihieronymiellaperuvianaiamaryllidaceae AT javiereortiz alkaloidprofileanticholinesteraseandantioxidantactivitiesandsexualpropagationinihieronymiellaperuvianaiamaryllidaceae AT lucianartallini alkaloidprofileanticholinesteraseandantioxidantactivitiesandsexualpropagationinihieronymiellaperuvianaiamaryllidaceae AT lauratorrasclaveria alkaloidprofileanticholinesteraseandantioxidantactivitiesandsexualpropagationinihieronymiellaperuvianaiamaryllidaceae AT jaumebastida alkaloidprofileanticholinesteraseandantioxidantactivitiesandsexualpropagationinihieronymiellaperuvianaiamaryllidaceae AT lorenacelinaluna alkaloidprofileanticholinesteraseandantioxidantactivitiesandsexualpropagationinihieronymiellaperuvianaiamaryllidaceae AT gabrielaeferesin alkaloidprofileanticholinesteraseandantioxidantactivitiesandsexualpropagationinihieronymiellaperuvianaiamaryllidaceae |