Comparative transcriptome-based identification and expression analysis of ATP-binding cassette (ABC) transporters reveals a likely role in the transport of β-caryophyllene and response to abiotic stress in Brassica campestris
β-caryophyllene has physiological activities such as antibacterial, antiedemic, anti-inflammatory, antitumor, and fungicidal. Previous research has focused on the synthesis of β-caryophyllene, however little information is available about its involvement in β-caryophyllene transport. ATP-binding cas...
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Maximum Academic Press
2023-01-01
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| Series: | Vegetable Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/VR-2023-0013 |
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| author | Haibin Wang Chen Zong Yibo Bai Shuilin Yuan Yan Li Tiantian Han Huanhuan Xu Chunmei Hu Xilin Hou Ying Li |
| author_facet | Haibin Wang Chen Zong Yibo Bai Shuilin Yuan Yan Li Tiantian Han Huanhuan Xu Chunmei Hu Xilin Hou Ying Li |
| author_sort | Haibin Wang |
| collection | DOAJ |
| description | β-caryophyllene has physiological activities such as antibacterial, antiedemic, anti-inflammatory, antitumor, and fungicidal. Previous research has focused on the synthesis of β-caryophyllene, however little information is available about its involvement in β-caryophyllene transport. ATP-binding cassette (ABC) transporters are involved in the transport of multiple substrates such as amino acids, terpenes, and heavy metals. Herein, we present a genome-wide comparative transcriptome analysis between non-β-caryophyllene cultivars ('SZQ') and high β-caryophyllene cultivars ('XQC') to identify potential ABC functional gene activities that occur during plant development. This article analyzes potential ABC transporters that may transport β-caryophyllene by comparative transcriptome and GC-MS, and explores the genetic structure, evolutionary relationship, cis-acting element analysis, and transcription patterns of these transporters under different treatment conditions. Phylogenetic and cis-acting element analysis indicated that eight genes in 'SZQ' and 'XQC' belonged to the ABCC, ABCG, and ABCE subfamilies and had the highest number of light-responsive elements and MYB binding sites. The RNA-seq and qRT-PCR results showed that eight ABC genes were expressed in a tissue- and development-specific manner, while their abundance apparently varied when plants were exposed to different treatment conditions. Pearson correlation analysis showed that ABCG18 is a potential carrier of β-caryophyllene, which might participate in substance transport in leaves and petioles. In summary, these observations open up new future research directions for β-caryophyllene transport and provide insight into ABC family genes in Brassica campestris. |
| format | Article |
| id | doaj-art-7c62ddd9ede841dba2d8a5d4f33c07bc |
| institution | OA Journals |
| issn | 2769-0520 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Maximum Academic Press |
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| series | Vegetable Research |
| spelling | doaj-art-7c62ddd9ede841dba2d8a5d4f33c07bc2025-08-20T02:12:16ZengMaximum Academic PressVegetable Research2769-05202023-01-01311910.48130/VR-2023-0013VR-2023-0013Comparative transcriptome-based identification and expression analysis of ATP-binding cassette (ABC) transporters reveals a likely role in the transport of β-caryophyllene and response to abiotic stress in Brassica campestrisHaibin Wang0Chen Zong1Yibo Bai2Shuilin Yuan3Yan Li4Tiantian Han5Huanhuan Xu6Chunmei Hu7Xilin Hou8Ying Li9National Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, Chinaβ-caryophyllene has physiological activities such as antibacterial, antiedemic, anti-inflammatory, antitumor, and fungicidal. Previous research has focused on the synthesis of β-caryophyllene, however little information is available about its involvement in β-caryophyllene transport. ATP-binding cassette (ABC) transporters are involved in the transport of multiple substrates such as amino acids, terpenes, and heavy metals. Herein, we present a genome-wide comparative transcriptome analysis between non-β-caryophyllene cultivars ('SZQ') and high β-caryophyllene cultivars ('XQC') to identify potential ABC functional gene activities that occur during plant development. This article analyzes potential ABC transporters that may transport β-caryophyllene by comparative transcriptome and GC-MS, and explores the genetic structure, evolutionary relationship, cis-acting element analysis, and transcription patterns of these transporters under different treatment conditions. Phylogenetic and cis-acting element analysis indicated that eight genes in 'SZQ' and 'XQC' belonged to the ABCC, ABCG, and ABCE subfamilies and had the highest number of light-responsive elements and MYB binding sites. The RNA-seq and qRT-PCR results showed that eight ABC genes were expressed in a tissue- and development-specific manner, while their abundance apparently varied when plants were exposed to different treatment conditions. Pearson correlation analysis showed that ABCG18 is a potential carrier of β-caryophyllene, which might participate in substance transport in leaves and petioles. In summary, these observations open up new future research directions for β-caryophyllene transport and provide insight into ABC family genes in Brassica campestris.https://www.maxapress.com/article/doi/10.48130/VR-2023-0013β-caryophylleneabc proteinsexpression profiletransport |
| spellingShingle | Haibin Wang Chen Zong Yibo Bai Shuilin Yuan Yan Li Tiantian Han Huanhuan Xu Chunmei Hu Xilin Hou Ying Li Comparative transcriptome-based identification and expression analysis of ATP-binding cassette (ABC) transporters reveals a likely role in the transport of β-caryophyllene and response to abiotic stress in Brassica campestris Vegetable Research β-caryophyllene abc proteins expression profile transport |
| title | Comparative transcriptome-based identification and expression analysis of ATP-binding cassette (ABC) transporters reveals a likely role in the transport of β-caryophyllene and response to abiotic stress in Brassica campestris |
| title_full | Comparative transcriptome-based identification and expression analysis of ATP-binding cassette (ABC) transporters reveals a likely role in the transport of β-caryophyllene and response to abiotic stress in Brassica campestris |
| title_fullStr | Comparative transcriptome-based identification and expression analysis of ATP-binding cassette (ABC) transporters reveals a likely role in the transport of β-caryophyllene and response to abiotic stress in Brassica campestris |
| title_full_unstemmed | Comparative transcriptome-based identification and expression analysis of ATP-binding cassette (ABC) transporters reveals a likely role in the transport of β-caryophyllene and response to abiotic stress in Brassica campestris |
| title_short | Comparative transcriptome-based identification and expression analysis of ATP-binding cassette (ABC) transporters reveals a likely role in the transport of β-caryophyllene and response to abiotic stress in Brassica campestris |
| title_sort | comparative transcriptome based identification and expression analysis of atp binding cassette abc transporters reveals a likely role in the transport of β caryophyllene and response to abiotic stress in brassica campestris |
| topic | β-caryophyllene abc proteins expression profile transport |
| url | https://www.maxapress.com/article/doi/10.48130/VR-2023-0013 |
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