Comparative transcriptome-based identification and expression analysis of ATP-binding cassette (ABC) transporters reveals a likely role in the transport of β-caryophyllene and response to abiotic stress in Brassica campestris
β-caryophyllene has physiological activities such as antibacterial, antiedemic, anti-inflammatory, antitumor, and fungicidal. Previous research has focused on the synthesis of β-caryophyllene, however little information is available about its involvement in β-caryophyllene transport. ATP-binding cas...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Maximum Academic Press
2023-01-01
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| Series: | Vegetable Research |
| Subjects: | |
| Online Access: | https://www.maxapress.com/article/doi/10.48130/VR-2023-0013 |
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| Summary: | β-caryophyllene has physiological activities such as antibacterial, antiedemic, anti-inflammatory, antitumor, and fungicidal. Previous research has focused on the synthesis of β-caryophyllene, however little information is available about its involvement in β-caryophyllene transport. ATP-binding cassette (ABC) transporters are involved in the transport of multiple substrates such as amino acids, terpenes, and heavy metals. Herein, we present a genome-wide comparative transcriptome analysis between non-β-caryophyllene cultivars ('SZQ') and high β-caryophyllene cultivars ('XQC') to identify potential ABC functional gene activities that occur during plant development. This article analyzes potential ABC transporters that may transport β-caryophyllene by comparative transcriptome and GC-MS, and explores the genetic structure, evolutionary relationship, cis-acting element analysis, and transcription patterns of these transporters under different treatment conditions. Phylogenetic and cis-acting element analysis indicated that eight genes in 'SZQ' and 'XQC' belonged to the ABCC, ABCG, and ABCE subfamilies and had the highest number of light-responsive elements and MYB binding sites. The RNA-seq and qRT-PCR results showed that eight ABC genes were expressed in a tissue- and development-specific manner, while their abundance apparently varied when plants were exposed to different treatment conditions. Pearson correlation analysis showed that ABCG18 is a potential carrier of β-caryophyllene, which might participate in substance transport in leaves and petioles. In summary, these observations open up new future research directions for β-caryophyllene transport and provide insight into ABC family genes in Brassica campestris. |
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| ISSN: | 2769-0520 |