Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations
Objective. The study aimed at locating and quantifying Toll Like Receptor (TLR) 3, 7, 8, and 9 expression in kidney of patients with lupus nephritis (LN) and correlating them with clinicopathological features. Methods. Kidney sections from 26 LN patients and 4 controls were analyzed by immunohistoch...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/7697592 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832555474674778112 |
|---|---|
| author | Fabrizio Conti Francesca Romana Spinelli Simona Truglia Francesca Miranda Cristiano Alessandri Fulvia Ceccarelli Michele Bombardieri Konstantinos Giannakakis Guido Valesini |
| author_facet | Fabrizio Conti Francesca Romana Spinelli Simona Truglia Francesca Miranda Cristiano Alessandri Fulvia Ceccarelli Michele Bombardieri Konstantinos Giannakakis Guido Valesini |
| author_sort | Fabrizio Conti |
| collection | DOAJ |
| description | Objective. The study aimed at locating and quantifying Toll Like Receptor (TLR) 3, 7, 8, and 9 expression in kidney of patients with lupus nephritis (LN) and correlating them with clinicopathological features. Methods. Kidney sections from 26 LN patients and 4 controls were analyzed by immunohistochemistry using anti-human TLR3, TLR7, TLR8, and TLR9 polyclonal antibodies; the number of TLR-positive nuclei/mm2 was evaluated on digitalized images. Results. Compared to controls, LN showed a significantly higher amount of glomerular and tubulointerstitial TLR9 (p=0.003 and p=0.007), whole and tubulointerstitial TLR3 (p=0.026 and p=0.031), and a higher tubulointerstitial TLR7 (p=0.022). TLR9 positively correlated with activity index (p=0.0063) and tubular TLR7 with chronicity index (p=0.026). TLR9 positively correlated with Renal-SLEDAI (p=0.01). Conclusions. This is the first study quantifying kidney expressions of TLRs in LN patients; the results show an overexpression of TLR3, TLR7, and TLR9 and demonstrate a correlation with clinicopathological indices supporting a role of these mediators in the pathogenesis of LN. |
| format | Article |
| id | doaj-art-7c54e5253b5242369e25ea1cf009af98 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-7c54e5253b5242369e25ea1cf009af982025-02-03T05:48:10ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/76975927697592Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological CorrelationsFabrizio Conti0Francesca Romana Spinelli1Simona Truglia2Francesca Miranda3Cristiano Alessandri4Fulvia Ceccarelli5Michele Bombardieri6Konstantinos Giannakakis7Guido Valesini8Department of Internal Medicine and Medical Specialties-Rheumatology, Sapienza University of Rome, 00161 Rome, ItalyDepartment of Internal Medicine and Medical Specialties-Rheumatology, Sapienza University of Rome, 00161 Rome, ItalyDepartment of Internal Medicine and Medical Specialties-Rheumatology, Sapienza University of Rome, 00161 Rome, ItalyDepartment of Internal Medicine and Medical Specialties-Rheumatology, Sapienza University of Rome, 00161 Rome, ItalyDepartment of Internal Medicine and Medical Specialties-Rheumatology, Sapienza University of Rome, 00161 Rome, ItalyDepartment of Internal Medicine and Medical Specialties-Rheumatology, Sapienza University of Rome, 00161 Rome, ItalyCentre for Experimental Medicine and Rheumatology, William Harvey Research Institute, Queen Mary University of London, London EC1M 6BQ, UKDepartment of Radiology, Oncology and Pathology, Sapienza University of Rome, 00161 Rome, ItalyDepartment of Internal Medicine and Medical Specialties-Rheumatology, Sapienza University of Rome, 00161 Rome, ItalyObjective. The study aimed at locating and quantifying Toll Like Receptor (TLR) 3, 7, 8, and 9 expression in kidney of patients with lupus nephritis (LN) and correlating them with clinicopathological features. Methods. Kidney sections from 26 LN patients and 4 controls were analyzed by immunohistochemistry using anti-human TLR3, TLR7, TLR8, and TLR9 polyclonal antibodies; the number of TLR-positive nuclei/mm2 was evaluated on digitalized images. Results. Compared to controls, LN showed a significantly higher amount of glomerular and tubulointerstitial TLR9 (p=0.003 and p=0.007), whole and tubulointerstitial TLR3 (p=0.026 and p=0.031), and a higher tubulointerstitial TLR7 (p=0.022). TLR9 positively correlated with activity index (p=0.0063) and tubular TLR7 with chronicity index (p=0.026). TLR9 positively correlated with Renal-SLEDAI (p=0.01). Conclusions. This is the first study quantifying kidney expressions of TLRs in LN patients; the results show an overexpression of TLR3, TLR7, and TLR9 and demonstrate a correlation with clinicopathological indices supporting a role of these mediators in the pathogenesis of LN.http://dx.doi.org/10.1155/2016/7697592 |
| spellingShingle | Fabrizio Conti Francesca Romana Spinelli Simona Truglia Francesca Miranda Cristiano Alessandri Fulvia Ceccarelli Michele Bombardieri Konstantinos Giannakakis Guido Valesini Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations Mediators of Inflammation |
| title | Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations |
| title_full | Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations |
| title_fullStr | Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations |
| title_full_unstemmed | Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations |
| title_short | Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations |
| title_sort | kidney expression of toll like receptors in lupus nephritis quantification and clinicopathological correlations |
| url | http://dx.doi.org/10.1155/2016/7697592 |
| work_keys_str_mv | AT fabrizioconti kidneyexpressionoftolllikereceptorsinlupusnephritisquantificationandclinicopathologicalcorrelations AT francescaromanaspinelli kidneyexpressionoftolllikereceptorsinlupusnephritisquantificationandclinicopathologicalcorrelations AT simonatruglia kidneyexpressionoftolllikereceptorsinlupusnephritisquantificationandclinicopathologicalcorrelations AT francescamiranda kidneyexpressionoftolllikereceptorsinlupusnephritisquantificationandclinicopathologicalcorrelations AT cristianoalessandri kidneyexpressionoftolllikereceptorsinlupusnephritisquantificationandclinicopathologicalcorrelations AT fulviaceccarelli kidneyexpressionoftolllikereceptorsinlupusnephritisquantificationandclinicopathologicalcorrelations AT michelebombardieri kidneyexpressionoftolllikereceptorsinlupusnephritisquantificationandclinicopathologicalcorrelations AT konstantinosgiannakakis kidneyexpressionoftolllikereceptorsinlupusnephritisquantificationandclinicopathologicalcorrelations AT guidovalesini kidneyexpressionoftolllikereceptorsinlupusnephritisquantificationandclinicopathologicalcorrelations |