β-glucan attenuates intestinal ischemia-reperfusion injury in mice by promoting glucagon-like peptide-1 secretion
Objective To investigate the protective effect of β-glucan (BG) against intestinal ischemia reperfusion (II/R) injury by regulating the secretion of glucagon-like peptide-1 (GLP-1). Methods Male C57BL/6 mice (6~8 weeks old) were subjected, and finally, the experiments had sham group, II/R group,...
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Editorial Office of Journal of Army Medical University
2025-01-01
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| Series: | 陆军军医大学学报 |
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| author | WANG Wei HAN Ben SUN Lihua |
| author_facet | WANG Wei HAN Ben SUN Lihua |
| author_sort | WANG Wei |
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| description | Objective To investigate the protective effect of β-glucan (BG) against intestinal ischemia reperfusion (II/R) injury by regulating the secretion of glucagon-like peptide-1 (GLP-1). Methods Male C57BL/6 mice (6~8 weeks old) were subjected, and finally, the experiments had sham group, II/R group, II/R+BG group (0.1 mg/mL BG in drinking water for 2 weeks before modeling), II/R+liraglutide (LLT, GLP-1 analogue) group (0.2 μg/g LLT injected every 12 hours for 3 consecutive days before modeling), and II/R+BG+Ex9-39 (GLP-1R antagonist) group (intraperitoneal injection of 2 μg/g Ex9-39 1 h before modeling). After modeling, HE staining was used to observe intestinal morphological changes, and RT-qPCR and Western blotting were employed to evaluate the molecules (Occludin, ZO-1 and Claudin-1) related to intestinal barrier damage. The effect of 0.1 mg/mL BG treatment on the GLP-1 level in the serum and intestinal tissues of normal mice was determined with ELISA and immunofluorescence assay, respectively, and RT-PCR for the molecules related to GLP-1 expression (Gcg, Pcsk1/2, GIP and Foxa2). The effects of LLT and Ex9-39 pretreatment on intestinal morphology and intestinal barrier damage were also determined by morphological observation and expression levels of related molecules. Results II/R induced significant decreases in the mRNA levels of Occludin, ZO-1 and Claudin-1 and increase in Chiu's score when compared with sham control mice (P<0.05). While, the mRNA levels of the 3 molecules were obviously higher and the Chiu's score was lower in the II/R+BG group than the II/R group (P<0.05). BG pretreatment induced notably enhanced secretion of GLP-1 in the serum and intestinal tract of normal mice, and improved the mRNA expression of GLP-1-related molecules (P<0.05). The intervention of GLP-1 analogue LLT could attenuate the II/R damage and decreased Chiu's score, with statistical difference in comparison with the II/R group (P<0.05). GLP-1 receptor antagonist Ex9-39 reversed the protective effects of BG pretreatment against II/R damage, with notably differences in the expression of Occludin, ZO-1 and Claudin-1 and Chiu's score (P<0.05). Conclusion BG can attenuate intestinal mucosal and functional injury after II/R by promoting intestinal GLP-1 secretion.
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| format | Article |
| id | doaj-art-7c33808a8ebf4414ba40919948640f06 |
| institution | Kabale University |
| issn | 2097-0927 |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | Editorial Office of Journal of Army Medical University |
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| series | 陆军军医大学学报 |
| spelling | doaj-art-7c33808a8ebf4414ba40919948640f062025-01-23T07:52:22ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272025-01-0147211212110.16016/j.2097-0927.202409020β-glucan attenuates intestinal ischemia-reperfusion injury in mice by promoting glucagon-like peptide-1 secretionWANG Wei0HAN Ben1SUN Lihua2Department of Nutrition, Second Affiliated Hospital， Army Medical University （Third Military Medical University）， Chongqing， ChinaDepartment of Nutrition, Second Affiliated Hospital， Army Medical University （Third Military Medical University）， Chongqing， ChinaDepartment of General Surgery, Second Affiliated Hospital， Army Medical University （Third Military Medical University）， Chongqing， ChinaObjective To investigate the protective effect of β-glucan (BG) against intestinal ischemia reperfusion (II/R) injury by regulating the secretion of glucagon-like peptide-1 (GLP-1). Methods Male C57BL/6 mice (6~8 weeks old) were subjected, and finally, the experiments had sham group, II/R group, II/R+BG group (0.1 mg/mL BG in drinking water for 2 weeks before modeling), II/R+liraglutide (LLT, GLP-1 analogue) group (0.2 μg/g LLT injected every 12 hours for 3 consecutive days before modeling), and II/R+BG+Ex9-39 (GLP-1R antagonist) group (intraperitoneal injection of 2 μg/g Ex9-39 1 h before modeling). After modeling, HE staining was used to observe intestinal morphological changes, and RT-qPCR and Western blotting were employed to evaluate the molecules (Occludin, ZO-1 and Claudin-1) related to intestinal barrier damage. The effect of 0.1 mg/mL BG treatment on the GLP-1 level in the serum and intestinal tissues of normal mice was determined with ELISA and immunofluorescence assay, respectively, and RT-PCR for the molecules related to GLP-1 expression (Gcg, Pcsk1/2, GIP and Foxa2). The effects of LLT and Ex9-39 pretreatment on intestinal morphology and intestinal barrier damage were also determined by morphological observation and expression levels of related molecules. Results II/R induced significant decreases in the mRNA levels of Occludin, ZO-1 and Claudin-1 and increase in Chiu's score when compared with sham control mice (P<0.05). While, the mRNA levels of the 3 molecules were obviously higher and the Chiu's score was lower in the II/R+BG group than the II/R group (P<0.05). BG pretreatment induced notably enhanced secretion of GLP-1 in the serum and intestinal tract of normal mice, and improved the mRNA expression of GLP-1-related molecules (P<0.05). The intervention of GLP-1 analogue LLT could attenuate the II/R damage and decreased Chiu's score, with statistical difference in comparison with the II/R group (P<0.05). GLP-1 receptor antagonist Ex9-39 reversed the protective effects of BG pretreatment against II/R damage, with notably differences in the expression of Occludin, ZO-1 and Claudin-1 and Chiu's score (P<0.05). Conclusion BG can attenuate intestinal mucosal and functional injury after II/R by promoting intestinal GLP-1 secretion. https://aammt.tmmu.edu.cn/html/202409020.htmlintestinal ischaemia-reperfusion injuryβ-glucanglucagon-like peptide-1intestinal barrier |
| spellingShingle | WANG Wei HAN Ben SUN Lihua β-glucan attenuates intestinal ischemia-reperfusion injury in mice by promoting glucagon-like peptide-1 secretion 陆军军医大学学报 intestinal ischaemia-reperfusion injury β-glucan glucagon-like peptide-1 intestinal barrier |
| title | β-glucan attenuates intestinal ischemia-reperfusion injury in mice by promoting glucagon-like peptide-1 secretion |
| title_full | β-glucan attenuates intestinal ischemia-reperfusion injury in mice by promoting glucagon-like peptide-1 secretion |
| title_fullStr | β-glucan attenuates intestinal ischemia-reperfusion injury in mice by promoting glucagon-like peptide-1 secretion |
| title_full_unstemmed | β-glucan attenuates intestinal ischemia-reperfusion injury in mice by promoting glucagon-like peptide-1 secretion |
| title_short | β-glucan attenuates intestinal ischemia-reperfusion injury in mice by promoting glucagon-like peptide-1 secretion |
| title_sort | β glucan attenuates intestinal ischemia reperfusion injury in mice by promoting glucagon like peptide 1 secretion |
| topic | intestinal ischaemia-reperfusion injury β-glucan glucagon-like peptide-1 intestinal barrier |
| url | https://aammt.tmmu.edu.cn/html/202409020.html |
| work_keys_str_mv | AT wangwei bglucanattenuatesintestinalischemiareperfusioninjuryinmicebypromotingglucagonlikepeptide1secretion AT hanben bglucanattenuatesintestinalischemiareperfusioninjuryinmicebypromotingglucagonlikepeptide1secretion AT sunlihua bglucanattenuatesintestinalischemiareperfusioninjuryinmicebypromotingglucagonlikepeptide1secretion |