Spectrophotometric Determination of Calcium with Dibromo-p-methylsulfonazo
Calcium is a necessary trace element in the human body, and its low or high content in the human body is not appropriate. It is very meaningful to study the level of calcium in the human body and to determine calcium content in blood. As people take calcium through food, the determination of calcium...
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Wiley
2020-01-01
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Series: | Journal of Chemistry |
Online Access: | http://dx.doi.org/10.1155/2020/9232385 |
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author | Xiao-Dong Li Qing-Zhou Zhai |
author_facet | Xiao-Dong Li Qing-Zhou Zhai |
author_sort | Xiao-Dong Li |
collection | DOAJ |
description | Calcium is a necessary trace element in the human body, and its low or high content in the human body is not appropriate. It is very meaningful to study the level of calcium in the human body and to determine calcium content in blood. As people take calcium through food, the determination of calcium content in certain tea and calcium supplements is of great significance. In this study, a new method for the determination of calcium was established using dibromo-p-methylsulfonazo as a chromogenic agent. In this article, the optimal conditions for the color reaction of Ca2+ with dibromo-p-methylsulfonazo were established. In a hydrochloric acid medium, Ca2+ and dibromo-p-methylsulfonazo form a blue complex. At 624 nm maximum absorption wavelength, the apparent molar absorptivity for the determination of calcium is 3.32 × 104 L·mol−1·cm−1. Beer’s law is applied for 0–0.60 μg/mL. The method has already been used in the determination of calcium in blood serum, whole blood, tea leaf, and calcium fudge with satisfactory results. |
format | Article |
id | doaj-art-7c266092bccc4a12a1f62da4f1a75bf8 |
institution | Kabale University |
issn | 2090-9063 2090-9071 |
language | English |
publishDate | 2020-01-01 |
publisher | Wiley |
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series | Journal of Chemistry |
spelling | doaj-art-7c266092bccc4a12a1f62da4f1a75bf82025-02-03T06:05:14ZengWileyJournal of Chemistry2090-90632090-90712020-01-01202010.1155/2020/92323859232385Spectrophotometric Determination of Calcium with Dibromo-p-methylsulfonazoXiao-Dong Li0Qing-Zhou Zhai1Department of Basic Science, Jilin Jianzhu University, 5088 Xincheng Street, Changchun 130118, Jilin Province, ChinaResearch Center for Nanotechnology, South Campus, Changchun University of Science and Technology, 7186 Weixing Road, Changchun 130022, ChinaCalcium is a necessary trace element in the human body, and its low or high content in the human body is not appropriate. It is very meaningful to study the level of calcium in the human body and to determine calcium content in blood. As people take calcium through food, the determination of calcium content in certain tea and calcium supplements is of great significance. In this study, a new method for the determination of calcium was established using dibromo-p-methylsulfonazo as a chromogenic agent. In this article, the optimal conditions for the color reaction of Ca2+ with dibromo-p-methylsulfonazo were established. In a hydrochloric acid medium, Ca2+ and dibromo-p-methylsulfonazo form a blue complex. At 624 nm maximum absorption wavelength, the apparent molar absorptivity for the determination of calcium is 3.32 × 104 L·mol−1·cm−1. Beer’s law is applied for 0–0.60 μg/mL. The method has already been used in the determination of calcium in blood serum, whole blood, tea leaf, and calcium fudge with satisfactory results.http://dx.doi.org/10.1155/2020/9232385 |
spellingShingle | Xiao-Dong Li Qing-Zhou Zhai Spectrophotometric Determination of Calcium with Dibromo-p-methylsulfonazo Journal of Chemistry |
title | Spectrophotometric Determination of Calcium with Dibromo-p-methylsulfonazo |
title_full | Spectrophotometric Determination of Calcium with Dibromo-p-methylsulfonazo |
title_fullStr | Spectrophotometric Determination of Calcium with Dibromo-p-methylsulfonazo |
title_full_unstemmed | Spectrophotometric Determination of Calcium with Dibromo-p-methylsulfonazo |
title_short | Spectrophotometric Determination of Calcium with Dibromo-p-methylsulfonazo |
title_sort | spectrophotometric determination of calcium with dibromo p methylsulfonazo |
url | http://dx.doi.org/10.1155/2020/9232385 |
work_keys_str_mv | AT xiaodongli spectrophotometricdeterminationofcalciumwithdibromopmethylsulfonazo AT qingzhouzhai spectrophotometricdeterminationofcalciumwithdibromopmethylsulfonazo |