Revisiting Fat Graft Harvesting and Processing Technique to Optimize Its Regenerative Potential
Background:. The use of fat grafting has expanded to include cell and tissue regeneration, necessitating investigations to ensure the viability of stromal and adipose-derived mesenchymal stem cells (ASCs) within the transferred fat parcels. This study explored the impact of harvesting technique and...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer
2025-01-01
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| Series: | Plastic and Reconstructive Surgery, Global Open |
| Online Access: | http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000006420 |
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| author | Karina Karina, MD, PhD Johannes A. Biben, MD Krista Ekaputri, MD Grady Krisandi, BMed Imam Rosadi, MSc Siti Sobariah, BSc Irsyah Afini, MBiomed Tias Widyastuti, BSc Alfida Zakiyah, BSc Difky Ernanda, BSc |
| author_facet | Karina Karina, MD, PhD Johannes A. Biben, MD Krista Ekaputri, MD Grady Krisandi, BMed Imam Rosadi, MSc Siti Sobariah, BSc Irsyah Afini, MBiomed Tias Widyastuti, BSc Alfida Zakiyah, BSc Difky Ernanda, BSc |
| author_sort | Karina Karina, MD, PhD |
| collection | DOAJ |
| description | Background:. The use of fat grafting has expanded to include cell and tissue regeneration, necessitating investigations to ensure the viability of stromal and adipose-derived mesenchymal stem cells (ASCs) within the transferred fat parcels. This study explored the impact of harvesting technique and centrifugation on the viability of stromal cells and ASCs in lipoaspirate.
Methods:. Fat was harvested from patients undergoing fat grafting using 2 types of liposuction cannula: (A) a 3-mm blunt tip cannula with 3 smooth holes and (B) a 2.4-mm, sharp point port, multihole blunt tip cannula. Fat from cannula B underwent different processing methods: no centrifugation, 300g, 600g, and 900g centrifugation. Stromal cells were isolated, quantified, and evaluated for viability. ASCs were cultured from these samples to confirm survival.
Results:. Lipoaspirates from 21 patients were analyzed. The mean stromal cell counts were 0.937 × 109 ± 0.346 × 109/mL for cannula A and 0.734 × 109 ± 0.266 × 109/mL for cannula B (P = 0.684), with viabilities of 98.79% and 98.22% (P = 0.631), respectively. ASCs isolated and after 2-passage culture were also higher for cannula A. Stromal cell quantification and viability were lowest in the noncentrifuged group (P < 0.05) and highest in the 600g centrifugation group.
Conclusions:. Fat harvesting using cannulas A and B showed no significant difference in stromal cell yield or viability. Handheld syringe liposuction preserved stromal vascular fraction cell and ASC viability. Centrifugation at different speeds did not significantly affect stromal cell viability. |
| format | Article |
| id | doaj-art-7c21e3e3c0d14ecf94fb1d266fe32e52 |
| institution | Kabale University |
| issn | 2169-7574 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Plastic and Reconstructive Surgery, Global Open |
| spelling | doaj-art-7c21e3e3c0d14ecf94fb1d266fe32e522025-01-24T09:19:58ZengWolters KluwerPlastic and Reconstructive Surgery, Global Open2169-75742025-01-01131e642010.1097/GOX.0000000000006420202501000-00006Revisiting Fat Graft Harvesting and Processing Technique to Optimize Its Regenerative PotentialKarina Karina, MD, PhD0Johannes A. Biben, MD1Krista Ekaputri, MD2Grady Krisandi, BMed3Imam Rosadi, MSc4Siti Sobariah, BSc5Irsyah Afini, MBiomed6Tias Widyastuti, BSc7Alfida Zakiyah, BSc8Difky Ernanda, BSc9From the * Division of Plastic Surgery, Hayandra Clinic, Hayandra Peduli Foundation, Jakarta, IndonesiaFrom the * Division of Plastic Surgery, Hayandra Clinic, Hayandra Peduli Foundation, Jakarta, IndonesiaFrom the * Division of Plastic Surgery, Hayandra Clinic, Hayandra Peduli Foundation, Jakarta, Indonesia† HayandraLab, Hayandra Peduli Foundation, Jakarta, Indonesia† HayandraLab, Hayandra Peduli Foundation, Jakarta, Indonesia† HayandraLab, Hayandra Peduli Foundation, Jakarta, Indonesia† HayandraLab, Hayandra Peduli Foundation, Jakarta, Indonesia† HayandraLab, Hayandra Peduli Foundation, Jakarta, Indonesia† HayandraLab, Hayandra Peduli Foundation, Jakarta, Indonesia† HayandraLab, Hayandra Peduli Foundation, Jakarta, IndonesiaBackground:. The use of fat grafting has expanded to include cell and tissue regeneration, necessitating investigations to ensure the viability of stromal and adipose-derived mesenchymal stem cells (ASCs) within the transferred fat parcels. This study explored the impact of harvesting technique and centrifugation on the viability of stromal cells and ASCs in lipoaspirate. Methods:. Fat was harvested from patients undergoing fat grafting using 2 types of liposuction cannula: (A) a 3-mm blunt tip cannula with 3 smooth holes and (B) a 2.4-mm, sharp point port, multihole blunt tip cannula. Fat from cannula B underwent different processing methods: no centrifugation, 300g, 600g, and 900g centrifugation. Stromal cells were isolated, quantified, and evaluated for viability. ASCs were cultured from these samples to confirm survival. Results:. Lipoaspirates from 21 patients were analyzed. The mean stromal cell counts were 0.937 × 109 ± 0.346 × 109/mL for cannula A and 0.734 × 109 ± 0.266 × 109/mL for cannula B (P = 0.684), with viabilities of 98.79% and 98.22% (P = 0.631), respectively. ASCs isolated and after 2-passage culture were also higher for cannula A. Stromal cell quantification and viability were lowest in the noncentrifuged group (P < 0.05) and highest in the 600g centrifugation group. Conclusions:. Fat harvesting using cannulas A and B showed no significant difference in stromal cell yield or viability. Handheld syringe liposuction preserved stromal vascular fraction cell and ASC viability. Centrifugation at different speeds did not significantly affect stromal cell viability.http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000006420 |
| spellingShingle | Karina Karina, MD, PhD Johannes A. Biben, MD Krista Ekaputri, MD Grady Krisandi, BMed Imam Rosadi, MSc Siti Sobariah, BSc Irsyah Afini, MBiomed Tias Widyastuti, BSc Alfida Zakiyah, BSc Difky Ernanda, BSc Revisiting Fat Graft Harvesting and Processing Technique to Optimize Its Regenerative Potential Plastic and Reconstructive Surgery, Global Open |
| title | Revisiting Fat Graft Harvesting and Processing Technique to Optimize Its Regenerative Potential |
| title_full | Revisiting Fat Graft Harvesting and Processing Technique to Optimize Its Regenerative Potential |
| title_fullStr | Revisiting Fat Graft Harvesting and Processing Technique to Optimize Its Regenerative Potential |
| title_full_unstemmed | Revisiting Fat Graft Harvesting and Processing Technique to Optimize Its Regenerative Potential |
| title_short | Revisiting Fat Graft Harvesting and Processing Technique to Optimize Its Regenerative Potential |
| title_sort | revisiting fat graft harvesting and processing technique to optimize its regenerative potential |
| url | http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000006420 |
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