L-Carnitine Alleviates the Myocardial Infarction and Left Ventricular Remodeling through Bax/Bcl-2 Signal Pathway
Purpose. L-carnitine (LC) is considered to have good therapeutic potential for myocardial infarction (MI), but its mechanism has not been clarified. The aim of the study is to elucidate the cardioprotective effects of LC in mice following MI and related mechanisms. Methods. ICR mice were treated wit...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Cardiovascular Therapeutics |
| Online Access: | http://dx.doi.org/10.1155/2022/9615674 |
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| author | Hao-Ran Li Xiao-Ming Zheng Yan Liu Jing-Hui Tian Jie-Jian Kou Jun-Zhuo Shi Xiao-Bin Pang Xin-Mei Xie Yu Yan |
| author_facet | Hao-Ran Li Xiao-Ming Zheng Yan Liu Jing-Hui Tian Jie-Jian Kou Jun-Zhuo Shi Xiao-Bin Pang Xin-Mei Xie Yu Yan |
| author_sort | Hao-Ran Li |
| collection | DOAJ |
| description | Purpose. L-carnitine (LC) is considered to have good therapeutic potential for myocardial infarction (MI), but its mechanism has not been clarified. The aim of the study is to elucidate the cardioprotective effects of LC in mice following MI and related mechanisms. Methods. ICR mice were treated with LC for 2 weeks after induction of MI with ligation of left anterior descending artery. Electrocardiographic (ECG) recording and echocardiography were used to evaluate cardiac function. H&E staining, TTC staining, and Masson staining were performed for morphological analysis and cardiac fibrosis. ELISA and immunofluorescence were utilized to detect biomarkers and inflammatory mediators. The key proteins in the Bax/Bcl-2 signaling pathway were also examined by Western blot. Results. Both echocardiography and histological measurement showed an improvement in cardiac function and morphology. Biomarkers such as LDH, NT-proBNP, cTnT, and AST, as well as the inflammatory cytokines IL-1β, IL-6, and TNF-α, were decreased in plasma of mice receiving LC treatment after myocardial injury. In addition, the expression of α-SMA as well as the key proteins in the Bax/Bcl-2 signaling pathway in cardiac myocardium were much lower in mice with LC treatment compared to those without after MI. Conclusions. Our data suggest that LC can effectively ameliorate left ventricular (LV) remodeling after MI, and its beneficial effects on myocardial function and remodeling may be attributable at least in part to anti-inflammatory and inhibition of the Bax/Bcl-2 apoptotic signaling pathway. |
| format | Article |
| id | doaj-art-7c0093ed6b3343ceaf580fdac167aab8 |
| institution | Kabale University |
| issn | 1755-5922 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cardiovascular Therapeutics |
| spelling | doaj-art-7c0093ed6b3343ceaf580fdac167aab82025-02-03T05:53:36ZengWileyCardiovascular Therapeutics1755-59222022-01-01202210.1155/2022/9615674L-Carnitine Alleviates the Myocardial Infarction and Left Ventricular Remodeling through Bax/Bcl-2 Signal PathwayHao-Ran Li0Xiao-Ming Zheng1Yan Liu2Jing-Hui Tian3Jie-Jian Kou4Jun-Zhuo Shi5Xiao-Bin Pang6Xin-Mei Xie7Yu Yan8School of PharmacySchool of PharmacyDepartment of Nuclear MedicineDepartment of PharmacyDepartment of PharmacySchool of PharmacySchool of PharmacySchool of PharmacyDepartment of Pharmacy & Institute of Clinical Medical SciencesPurpose. L-carnitine (LC) is considered to have good therapeutic potential for myocardial infarction (MI), but its mechanism has not been clarified. The aim of the study is to elucidate the cardioprotective effects of LC in mice following MI and related mechanisms. Methods. ICR mice were treated with LC for 2 weeks after induction of MI with ligation of left anterior descending artery. Electrocardiographic (ECG) recording and echocardiography were used to evaluate cardiac function. H&E staining, TTC staining, and Masson staining were performed for morphological analysis and cardiac fibrosis. ELISA and immunofluorescence were utilized to detect biomarkers and inflammatory mediators. The key proteins in the Bax/Bcl-2 signaling pathway were also examined by Western blot. Results. Both echocardiography and histological measurement showed an improvement in cardiac function and morphology. Biomarkers such as LDH, NT-proBNP, cTnT, and AST, as well as the inflammatory cytokines IL-1β, IL-6, and TNF-α, were decreased in plasma of mice receiving LC treatment after myocardial injury. In addition, the expression of α-SMA as well as the key proteins in the Bax/Bcl-2 signaling pathway in cardiac myocardium were much lower in mice with LC treatment compared to those without after MI. Conclusions. Our data suggest that LC can effectively ameliorate left ventricular (LV) remodeling after MI, and its beneficial effects on myocardial function and remodeling may be attributable at least in part to anti-inflammatory and inhibition of the Bax/Bcl-2 apoptotic signaling pathway.http://dx.doi.org/10.1155/2022/9615674 |
| spellingShingle | Hao-Ran Li Xiao-Ming Zheng Yan Liu Jing-Hui Tian Jie-Jian Kou Jun-Zhuo Shi Xiao-Bin Pang Xin-Mei Xie Yu Yan L-Carnitine Alleviates the Myocardial Infarction and Left Ventricular Remodeling through Bax/Bcl-2 Signal Pathway Cardiovascular Therapeutics |
| title | L-Carnitine Alleviates the Myocardial Infarction and Left Ventricular Remodeling through Bax/Bcl-2 Signal Pathway |
| title_full | L-Carnitine Alleviates the Myocardial Infarction and Left Ventricular Remodeling through Bax/Bcl-2 Signal Pathway |
| title_fullStr | L-Carnitine Alleviates the Myocardial Infarction and Left Ventricular Remodeling through Bax/Bcl-2 Signal Pathway |
| title_full_unstemmed | L-Carnitine Alleviates the Myocardial Infarction and Left Ventricular Remodeling through Bax/Bcl-2 Signal Pathway |
| title_short | L-Carnitine Alleviates the Myocardial Infarction and Left Ventricular Remodeling through Bax/Bcl-2 Signal Pathway |
| title_sort | l carnitine alleviates the myocardial infarction and left ventricular remodeling through bax bcl 2 signal pathway |
| url | http://dx.doi.org/10.1155/2022/9615674 |
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