Normal butanol fraction of Polygonum hydropiper L. flavonoids reduces inflammation caused by PCV2 infections in cell and mouse models
IntroductionThe normal butanol fraction of Polygonum hydropiper L. flavonoids (FNB) exhibits significant anti-inflammatory effects. This study investigated FNB's impact on inflammatory responses induced by Porcine circovirus type 2 (PCV2) in cell and mouse models.MethodsAn inflammatory model wa...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1539448/full |
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| author | Yu-heng Wei Shu-mian Zhou Shu-mian Zhou Shu-mian Zhou Wen Zhao Qi Chen Qiu-hua Wang Qiu-hua Wang Mei-ling Yu Ying-yi Wei Ting-jun Hu Ting-jun Hu Ting-jun Hu |
| author_facet | Yu-heng Wei Shu-mian Zhou Shu-mian Zhou Shu-mian Zhou Wen Zhao Qi Chen Qiu-hua Wang Qiu-hua Wang Mei-ling Yu Ying-yi Wei Ting-jun Hu Ting-jun Hu Ting-jun Hu |
| author_sort | Yu-heng Wei |
| collection | DOAJ |
| description | IntroductionThe normal butanol fraction of Polygonum hydropiper L. flavonoids (FNB) exhibits significant anti-inflammatory effects. This study investigated FNB's impact on inflammatory responses induced by Porcine circovirus type 2 (PCV2) in cell and mouse models.MethodsAn inflammatory model was established in RAW264.7 cells infected with varying PCV2 concentrations. And assigning both RAW264.7 cells and 108 SPF-grade KM mice to Control, PCV2, Rutin, and various dosages of FNB groups. Inflammatory factors such as Monocyte Chemoattractant Protein-1 (MCP-1), interleukin-6 (IL-6), IL-8, IL-10, Tumor Necrosis Factor-alpha (TNF-α), Reactive Oxygen Species (ROS), and Nitric Oxide (NO) were quantified using ELISA, RT-qPCR and immunohistochemistry.ResultsResults showed that a PCV2 titer of 104.5 TCID50/0.1 mL when applied to RAW264.7 cells effectively established an in vitro inflammatory model at 12 and 24 h post-infection. Following PCV2 infection, all the inflammatory factors displayed a significant increased both in culture supernatant and intracellular mRNA expression levels (p < 0.05 or p < 0.01), but these levels were reduced by FNB treatment (p < 0.05 or p < 0.01). In mouse sera post-PCV2 infection also showed elevated levels of IL-6, IL-8 IL-10, TNF-α, and MCP-1 (p < 0.05 or p < 0.01). Additionally, mRNA and protein levels for TNF-α, IL-8, IL-10, IL-6, and iNOS rose significantly in lung tissues (p < 0.01) but decreased with FNB treatment (p < 0.05 or p < 0.01).DiscussionThese findings suggest that FNB reduces inflammatory factor production and modulates the inflammatory response triggered by PCV2 infection, potentially enhancing host resistance against it. |
| format | Article |
| id | doaj-art-7bfd1763b21349f198219ab59a6d6e5a |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-02-01 |
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| series | Frontiers in Veterinary Science |
| spelling | doaj-art-7bfd1763b21349f198219ab59a6d6e5a2025-02-04T13:54:10ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-02-011210.3389/fvets.2025.15394481539448Normal butanol fraction of Polygonum hydropiper L. flavonoids reduces inflammation caused by PCV2 infections in cell and mouse modelsYu-heng Wei0Shu-mian Zhou1Shu-mian Zhou2Shu-mian Zhou3Wen Zhao4Qi Chen5Qiu-hua Wang6Qiu-hua Wang7Mei-ling Yu8Ying-yi Wei9Ting-jun Hu10Ting-jun Hu11Ting-jun Hu12College of Animal Science and Technology, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaGuangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, ChinaGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaGuangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, ChinaGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, ChinaIntroductionThe normal butanol fraction of Polygonum hydropiper L. flavonoids (FNB) exhibits significant anti-inflammatory effects. This study investigated FNB's impact on inflammatory responses induced by Porcine circovirus type 2 (PCV2) in cell and mouse models.MethodsAn inflammatory model was established in RAW264.7 cells infected with varying PCV2 concentrations. And assigning both RAW264.7 cells and 108 SPF-grade KM mice to Control, PCV2, Rutin, and various dosages of FNB groups. Inflammatory factors such as Monocyte Chemoattractant Protein-1 (MCP-1), interleukin-6 (IL-6), IL-8, IL-10, Tumor Necrosis Factor-alpha (TNF-α), Reactive Oxygen Species (ROS), and Nitric Oxide (NO) were quantified using ELISA, RT-qPCR and immunohistochemistry.ResultsResults showed that a PCV2 titer of 104.5 TCID50/0.1 mL when applied to RAW264.7 cells effectively established an in vitro inflammatory model at 12 and 24 h post-infection. Following PCV2 infection, all the inflammatory factors displayed a significant increased both in culture supernatant and intracellular mRNA expression levels (p < 0.05 or p < 0.01), but these levels were reduced by FNB treatment (p < 0.05 or p < 0.01). In mouse sera post-PCV2 infection also showed elevated levels of IL-6, IL-8 IL-10, TNF-α, and MCP-1 (p < 0.05 or p < 0.01). Additionally, mRNA and protein levels for TNF-α, IL-8, IL-10, IL-6, and iNOS rose significantly in lung tissues (p < 0.01) but decreased with FNB treatment (p < 0.05 or p < 0.01).DiscussionThese findings suggest that FNB reduces inflammatory factor production and modulates the inflammatory response triggered by PCV2 infection, potentially enhancing host resistance against it.https://www.frontiersin.org/articles/10.3389/fvets.2025.1539448/fullporcine circovirus type 2normal butanol fraction of Polygonum hydropiper L. flavonoidsinflammatory responseRAW264.7 cellsmodel establishment |
| spellingShingle | Yu-heng Wei Shu-mian Zhou Shu-mian Zhou Shu-mian Zhou Wen Zhao Qi Chen Qiu-hua Wang Qiu-hua Wang Mei-ling Yu Ying-yi Wei Ting-jun Hu Ting-jun Hu Ting-jun Hu Normal butanol fraction of Polygonum hydropiper L. flavonoids reduces inflammation caused by PCV2 infections in cell and mouse models Frontiers in Veterinary Science porcine circovirus type 2 normal butanol fraction of Polygonum hydropiper L. flavonoids inflammatory response RAW264.7 cells model establishment |
| title | Normal butanol fraction of Polygonum hydropiper L. flavonoids reduces inflammation caused by PCV2 infections in cell and mouse models |
| title_full | Normal butanol fraction of Polygonum hydropiper L. flavonoids reduces inflammation caused by PCV2 infections in cell and mouse models |
| title_fullStr | Normal butanol fraction of Polygonum hydropiper L. flavonoids reduces inflammation caused by PCV2 infections in cell and mouse models |
| title_full_unstemmed | Normal butanol fraction of Polygonum hydropiper L. flavonoids reduces inflammation caused by PCV2 infections in cell and mouse models |
| title_short | Normal butanol fraction of Polygonum hydropiper L. flavonoids reduces inflammation caused by PCV2 infections in cell and mouse models |
| title_sort | normal butanol fraction of polygonum hydropiper l flavonoids reduces inflammation caused by pcv2 infections in cell and mouse models |
| topic | porcine circovirus type 2 normal butanol fraction of Polygonum hydropiper L. flavonoids inflammatory response RAW264.7 cells model establishment |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1539448/full |
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