CAPS markers in plant biology
Cleaved Amplified Polymorphic Sequences (CAPS) markers are applicable in a wide range of tasksin plant biology. They have been developed for plant genetics and breeding and become especially useful. This mini-review analyzes information about the application of CAPS markers within the past3–5 years....
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-07-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/365 |
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| author | Y. N. Shavrukov |
| author_facet | Y. N. Shavrukov |
| author_sort | Y. N. Shavrukov |
| collection | DOAJ |
| description | Cleaved Amplified Polymorphic Sequences (CAPS) markers are applicable in a wide range of tasksin plant biology. They have been developed for plant genetics and breeding and become especially useful. This mini-review analyzes information about the application of CAPS markers within the past3–5 years. In the presented study, special attention is focused on CAPS markers linked with genes controlling important agricultural traits in different crops. The main principles of the developmentand analysis of CAPS markers, as well as advantages and disadvantages of this type of molecular markers, are briefly outlined in the beginning of this review. CAPS markers are based on PCR amplification of DNA fragments with specific primers followed by digestion with restriction enzymes and separation of the products in agarose gel. Functional CAPS markers can be developed on the known sequence of a gene of interest for the analyses of its structure, function, expression, and regulation. CAPS closely linked to the gene of interest are especially helpful for Marker-Assisted Selection, and they are widely used in the breeding of wheat, barley, soybean, potato, tomato, and other crops for tolerance to various pathogens. CAPS markers are often used for the preparation of genetic maps and fine mapping of studied genes. For some plants, first moleculargenetic maps were prepared using CAPS. This method was also successfully used for the mapping of both individual genes and QTLs controlling such important traits as plant growth habit, grain quality, and tolerance to pathogens in cereals, as well as the shape of tomato fruit. CAPS have important applications in the analyses of genetic polymorphism and phylogeny, particularly, in closely related species. Thus, CAPS are an effective tool for molecular-genetic research and plant breeding. |
| format | Article |
| id | doaj-art-7bef37798b424e5f9907eb29c9466c91 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2015-07-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-7bef37798b424e5f9907eb29c9466c912025-02-01T09:58:01ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-07-0119220521310.18699/VJ15.026344CAPS markers in plant biologyY. N. Shavrukov0School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Hartley Grove, AustraliaCleaved Amplified Polymorphic Sequences (CAPS) markers are applicable in a wide range of tasksin plant biology. They have been developed for plant genetics and breeding and become especially useful. This mini-review analyzes information about the application of CAPS markers within the past3–5 years. In the presented study, special attention is focused on CAPS markers linked with genes controlling important agricultural traits in different crops. The main principles of the developmentand analysis of CAPS markers, as well as advantages and disadvantages of this type of molecular markers, are briefly outlined in the beginning of this review. CAPS markers are based on PCR amplification of DNA fragments with specific primers followed by digestion with restriction enzymes and separation of the products in agarose gel. Functional CAPS markers can be developed on the known sequence of a gene of interest for the analyses of its structure, function, expression, and regulation. CAPS closely linked to the gene of interest are especially helpful for Marker-Assisted Selection, and they are widely used in the breeding of wheat, barley, soybean, potato, tomato, and other crops for tolerance to various pathogens. CAPS markers are often used for the preparation of genetic maps and fine mapping of studied genes. For some plants, first moleculargenetic maps were prepared using CAPS. This method was also successfully used for the mapping of both individual genes and QTLs controlling such important traits as plant growth habit, grain quality, and tolerance to pathogens in cereals, as well as the shape of tomato fruit. CAPS have important applications in the analyses of genetic polymorphism and phylogeny, particularly, in closely related species. Thus, CAPS are an effective tool for molecular-genetic research and plant breeding.https://vavilov.elpub.ru/jour/article/view/365plant genetics and breedingdna markerscapscleaved amplified polymorphic sequences |
| spellingShingle | Y. N. Shavrukov CAPS markers in plant biology Вавиловский журнал генетики и селекции plant genetics and breeding dna markers caps cleaved amplified polymorphic sequences |
| title | CAPS markers in plant biology |
| title_full | CAPS markers in plant biology |
| title_fullStr | CAPS markers in plant biology |
| title_full_unstemmed | CAPS markers in plant biology |
| title_short | CAPS markers in plant biology |
| title_sort | caps markers in plant biology |
| topic | plant genetics and breeding dna markers caps cleaved amplified polymorphic sequences |
| url | https://vavilov.elpub.ru/jour/article/view/365 |
| work_keys_str_mv | AT ynshavrukov capsmarkersinplantbiology |