Diagnostic Performance of Three ELISAs for Detection of Antibodies against SARS-CoV-2 in Human Samples
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection that causes coronavirus disease 2019 (COVID-19) is a disease with a high rate of transmission. Serological tests are important to perform surveys and to determine the immunological status of the population. Based on this, we eval...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2022/7754329 |
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| author | Cássio Meira Dahara Silva Ivanilson Santos Breno Barreto Vinícius Rocha Emanuelle Santos Bruna dos Reis Afrânio Evangelista Ricardo Ribeiro dos Santos Bruna Machado Guilherme Ribeiro Roberto Badaró Milena Soares |
| author_facet | Cássio Meira Dahara Silva Ivanilson Santos Breno Barreto Vinícius Rocha Emanuelle Santos Bruna dos Reis Afrânio Evangelista Ricardo Ribeiro dos Santos Bruna Machado Guilherme Ribeiro Roberto Badaró Milena Soares |
| author_sort | Cássio Meira |
| collection | DOAJ |
| description | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection that causes coronavirus disease 2019 (COVID-19) is a disease with a high rate of transmission. Serological tests are important to perform surveys and to determine the immunological status of the population. Based on this, we evaluated three enzyme-linked immunoassays (ELISAs) using different antigens from SARS-CoV-2 in a cohort of 161 patients. The performance of the ELISA developed for immunoglobulin G (IgG) measurement against SARS-CoV-2 was evaluated based on sensitivity, specificity, and accuracy. We found specificities of 0.98, 0.98, and 0.99 and sensitivities of 0.99, 0.91, and 0.87 for the nucleocapsid (N) protein, spike protein, and receptor binding domain (RBD) fraction, respectively. The accuracy assessment indicated the N protein (accuracy = 0.98) as the antigen most likely to give a correct diagnosis. Overall, the antibody responses were present for all three proteins in subjects with confirmed SARS-CoV-2 infections, showing a similar pattern of antibody production for different antigens. In summary, these highly sensitive and specific ELISAs, with a more competitive price, appear to be a valid approach for the serodiagnosis of COVID-19. |
| format | Article |
| id | doaj-art-7bb4bcda0fb64d868dc983c9395dede9 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-7bb4bcda0fb64d868dc983c9395dede92025-02-03T01:30:03ZengWileyThe Scientific World Journal1537-744X2022-01-01202210.1155/2022/7754329Diagnostic Performance of Three ELISAs for Detection of Antibodies against SARS-CoV-2 in Human SamplesCássio Meira0Dahara Silva1Ivanilson Santos2Breno Barreto3Vinícius Rocha4Emanuelle Santos5Bruna dos Reis6Afrânio Evangelista7Ricardo Ribeiro dos Santos8Bruna Machado9Guilherme Ribeiro10Roberto Badaró11Milena Soares12SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS)SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS)Gonçalo Moniz Institute Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA)Gonçalo Moniz Institute Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA)SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS)SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS)Gonçalo Moniz Institute Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA)SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS)SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS)SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS)Gonçalo Moniz Institute Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA)SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS)SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS)Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection that causes coronavirus disease 2019 (COVID-19) is a disease with a high rate of transmission. Serological tests are important to perform surveys and to determine the immunological status of the population. Based on this, we evaluated three enzyme-linked immunoassays (ELISAs) using different antigens from SARS-CoV-2 in a cohort of 161 patients. The performance of the ELISA developed for immunoglobulin G (IgG) measurement against SARS-CoV-2 was evaluated based on sensitivity, specificity, and accuracy. We found specificities of 0.98, 0.98, and 0.99 and sensitivities of 0.99, 0.91, and 0.87 for the nucleocapsid (N) protein, spike protein, and receptor binding domain (RBD) fraction, respectively. The accuracy assessment indicated the N protein (accuracy = 0.98) as the antigen most likely to give a correct diagnosis. Overall, the antibody responses were present for all three proteins in subjects with confirmed SARS-CoV-2 infections, showing a similar pattern of antibody production for different antigens. In summary, these highly sensitive and specific ELISAs, with a more competitive price, appear to be a valid approach for the serodiagnosis of COVID-19.http://dx.doi.org/10.1155/2022/7754329 |
| spellingShingle | Cássio Meira Dahara Silva Ivanilson Santos Breno Barreto Vinícius Rocha Emanuelle Santos Bruna dos Reis Afrânio Evangelista Ricardo Ribeiro dos Santos Bruna Machado Guilherme Ribeiro Roberto Badaró Milena Soares Diagnostic Performance of Three ELISAs for Detection of Antibodies against SARS-CoV-2 in Human Samples The Scientific World Journal |
| title | Diagnostic Performance of Three ELISAs for Detection of Antibodies against SARS-CoV-2 in Human Samples |
| title_full | Diagnostic Performance of Three ELISAs for Detection of Antibodies against SARS-CoV-2 in Human Samples |
| title_fullStr | Diagnostic Performance of Three ELISAs for Detection of Antibodies against SARS-CoV-2 in Human Samples |
| title_full_unstemmed | Diagnostic Performance of Three ELISAs for Detection of Antibodies against SARS-CoV-2 in Human Samples |
| title_short | Diagnostic Performance of Three ELISAs for Detection of Antibodies against SARS-CoV-2 in Human Samples |
| title_sort | diagnostic performance of three elisas for detection of antibodies against sars cov 2 in human samples |
| url | http://dx.doi.org/10.1155/2022/7754329 |
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