Elucidating the Mechanisms of Acquired Palbociclib Resistance via Comprehensive Metabolomics Profiling
Palbociclib is a cyclin-dependent kinase 4/6 inhibitor and a commonly used antitumor drug. Many cancers are susceptible to palbociclib resistance, however, the underlying metabolism mechanism and extent of resistance to palbociclib are unknown. In this study, LC-MS metabolomics was used to investiga...
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MDPI AG
2025-01-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/47/1/24 |
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| author | Lulu Yang Yajun Yue Zhendong Wang You Jiang Zhichao Xue Yongzhuo Zhang |
| author_facet | Lulu Yang Yajun Yue Zhendong Wang You Jiang Zhichao Xue Yongzhuo Zhang |
| author_sort | Lulu Yang |
| collection | DOAJ |
| description | Palbociclib is a cyclin-dependent kinase 4/6 inhibitor and a commonly used antitumor drug. Many cancers are susceptible to palbociclib resistance, however, the underlying metabolism mechanism and extent of resistance to palbociclib are unknown. In this study, LC-MS metabolomics was used to investigate the metabolite changes of colorectal cancer SW620 cells that were resistant to palbociclib. The study indicated that there were 76 metabolite expression differences between SW620 cells with palbociclib resistance and the parental SW620 cells involving amino acids, glutathione, ABC transporters, and so on. MetaboAnalyst 6.0 metabolic pathway analysis showed that arginine synthesis, β-alanine metabolism, and purine metabolism were disrupted. These results may provide potential clues to the metabolism mechanism of drug resistance in cancer cells that are resistant to palbociclib. Our study has the potential to contribute to the study of anti-palbociclib resistance. |
| format | Article |
| id | doaj-art-7ba16010201940ff8805dbd70aa7a4b0 |
| institution | Kabale University |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj-art-7ba16010201940ff8805dbd70aa7a4b02025-01-24T13:27:26ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-01-014712410.3390/cimb47010024Elucidating the Mechanisms of Acquired Palbociclib Resistance via Comprehensive Metabolomics ProfilingLulu Yang0Yajun Yue1Zhendong Wang2You Jiang3Zhichao Xue4Yongzhuo Zhang5Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, ChinaGeneral Management Department of Laboratory Base, National Institute of Metrology, Beijing 100029, ChinaCenter for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, ChinaTechnology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, ChinaTechnology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, ChinaCenter for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, ChinaPalbociclib is a cyclin-dependent kinase 4/6 inhibitor and a commonly used antitumor drug. Many cancers are susceptible to palbociclib resistance, however, the underlying metabolism mechanism and extent of resistance to palbociclib are unknown. In this study, LC-MS metabolomics was used to investigate the metabolite changes of colorectal cancer SW620 cells that were resistant to palbociclib. The study indicated that there were 76 metabolite expression differences between SW620 cells with palbociclib resistance and the parental SW620 cells involving amino acids, glutathione, ABC transporters, and so on. MetaboAnalyst 6.0 metabolic pathway analysis showed that arginine synthesis, β-alanine metabolism, and purine metabolism were disrupted. These results may provide potential clues to the metabolism mechanism of drug resistance in cancer cells that are resistant to palbociclib. Our study has the potential to contribute to the study of anti-palbociclib resistance.https://www.mdpi.com/1467-3045/47/1/24LC-MSSW620 cellsmetabolomicsdrug resistancecancer biology |
| spellingShingle | Lulu Yang Yajun Yue Zhendong Wang You Jiang Zhichao Xue Yongzhuo Zhang Elucidating the Mechanisms of Acquired Palbociclib Resistance via Comprehensive Metabolomics Profiling Current Issues in Molecular Biology LC-MS SW620 cells metabolomics drug resistance cancer biology |
| title | Elucidating the Mechanisms of Acquired Palbociclib Resistance via Comprehensive Metabolomics Profiling |
| title_full | Elucidating the Mechanisms of Acquired Palbociclib Resistance via Comprehensive Metabolomics Profiling |
| title_fullStr | Elucidating the Mechanisms of Acquired Palbociclib Resistance via Comprehensive Metabolomics Profiling |
| title_full_unstemmed | Elucidating the Mechanisms of Acquired Palbociclib Resistance via Comprehensive Metabolomics Profiling |
| title_short | Elucidating the Mechanisms of Acquired Palbociclib Resistance via Comprehensive Metabolomics Profiling |
| title_sort | elucidating the mechanisms of acquired palbociclib resistance via comprehensive metabolomics profiling |
| topic | LC-MS SW620 cells metabolomics drug resistance cancer biology |
| url | https://www.mdpi.com/1467-3045/47/1/24 |
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