Enhanced Bioactivity of Pomegranate Peel Extract following Controlled Release from CaCO3 Nanocrystals
Pomegranate peel extract is rich of interesting bioactive chemicals, principally phenolic compounds, which have shown antimicrobial, anticancer, and antioxidative properties. The aim of this work was to improve extract’ bioactivity through the adsorption on calcium carbonate nanocrystals. Nanocrysta...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2022/6341298 |
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| author | Francesca Baldassarre Viviana Vergaro Federica De Castro Francesca Biondo Gian Paolo Suranna Paride Papadia Francesco P. Fanizzi Domenico Rongai Giuseppe Ciccarella |
| author_facet | Francesca Baldassarre Viviana Vergaro Federica De Castro Francesca Biondo Gian Paolo Suranna Paride Papadia Francesco P. Fanizzi Domenico Rongai Giuseppe Ciccarella |
| author_sort | Francesca Baldassarre |
| collection | DOAJ |
| description | Pomegranate peel extract is rich of interesting bioactive chemicals, principally phenolic compounds, which have shown antimicrobial, anticancer, and antioxidative properties. The aim of this work was to improve extract’ bioactivity through the adsorption on calcium carbonate nanocrystals. Nanocrystals revealed as efficient tools for extract adsorption reaching 50% of loading efficiency. Controlled release of the contained metabolites under acidic pH has been found, as it was confirmed by quantitative assay and qualitative study through NMR analysis. Specific functionality of inorganic nanocarriers could be also tuned by biopolymeric coating. The resulting coated nanoformulations showed a great antimicrobial activity against B. cinerea fungus preventing strawberries disease better than a commercial fungicide. Furthermore, nanoformulations demonstrated a good antiproliferative activity in neuroblastoma and breast cancer cells carrying out a higher cytotoxic effect respect to free extract, confirming a crucial role of nanocarriers. Finally, pomegranate peel extract showed a very high radical scavenging ability, equal to ascorbic acid. Antioxidant activity, measured also in intracellular environment, highlighted a protective action of extract-adsorbed nanocrystals twice than free extract, providing a possible application for new nutraceutical formulations. |
| format | Article |
| id | doaj-art-7b9f1b0293d44ba38e15537923170fe1 |
| institution | Kabale University |
| issn | 1687-479X |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-7b9f1b0293d44ba38e15537923170fe12025-02-03T01:07:11ZengWileyBioinorganic Chemistry and Applications1687-479X2022-01-01202210.1155/2022/6341298Enhanced Bioactivity of Pomegranate Peel Extract following Controlled Release from CaCO3 NanocrystalsFrancesca Baldassarre0Viviana Vergaro1Federica De Castro2Francesca Biondo3Gian Paolo Suranna4Paride Papadia5Francesco P. Fanizzi6Domenico Rongai7Giuseppe Ciccarella8Department of Biological and Environmental SciencesInstitute of NanotechnologyDepartment of Biological and Environmental SciencesDepartment of Biological and Environmental SciencesInstitute of NanotechnologyDepartment of Biological and Environmental SciencesDepartment of Biological and Environmental SciencesCREA-IT PE-Research Centre for Engineering and Agro-Food ProcessingDepartment of Biological and Environmental SciencesPomegranate peel extract is rich of interesting bioactive chemicals, principally phenolic compounds, which have shown antimicrobial, anticancer, and antioxidative properties. The aim of this work was to improve extract’ bioactivity through the adsorption on calcium carbonate nanocrystals. Nanocrystals revealed as efficient tools for extract adsorption reaching 50% of loading efficiency. Controlled release of the contained metabolites under acidic pH has been found, as it was confirmed by quantitative assay and qualitative study through NMR analysis. Specific functionality of inorganic nanocarriers could be also tuned by biopolymeric coating. The resulting coated nanoformulations showed a great antimicrobial activity against B. cinerea fungus preventing strawberries disease better than a commercial fungicide. Furthermore, nanoformulations demonstrated a good antiproliferative activity in neuroblastoma and breast cancer cells carrying out a higher cytotoxic effect respect to free extract, confirming a crucial role of nanocarriers. Finally, pomegranate peel extract showed a very high radical scavenging ability, equal to ascorbic acid. Antioxidant activity, measured also in intracellular environment, highlighted a protective action of extract-adsorbed nanocrystals twice than free extract, providing a possible application for new nutraceutical formulations.http://dx.doi.org/10.1155/2022/6341298 |
| spellingShingle | Francesca Baldassarre Viviana Vergaro Federica De Castro Francesca Biondo Gian Paolo Suranna Paride Papadia Francesco P. Fanizzi Domenico Rongai Giuseppe Ciccarella Enhanced Bioactivity of Pomegranate Peel Extract following Controlled Release from CaCO3 Nanocrystals Bioinorganic Chemistry and Applications |
| title | Enhanced Bioactivity of Pomegranate Peel Extract following Controlled Release from CaCO3 Nanocrystals |
| title_full | Enhanced Bioactivity of Pomegranate Peel Extract following Controlled Release from CaCO3 Nanocrystals |
| title_fullStr | Enhanced Bioactivity of Pomegranate Peel Extract following Controlled Release from CaCO3 Nanocrystals |
| title_full_unstemmed | Enhanced Bioactivity of Pomegranate Peel Extract following Controlled Release from CaCO3 Nanocrystals |
| title_short | Enhanced Bioactivity of Pomegranate Peel Extract following Controlled Release from CaCO3 Nanocrystals |
| title_sort | enhanced bioactivity of pomegranate peel extract following controlled release from caco3 nanocrystals |
| url | http://dx.doi.org/10.1155/2022/6341298 |
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