DNA metabarcoding reveal hidden diversity of periphytic eukaryotes on marine Antarctic macroalgae
Abstract Polar marine macroalgae thrive in extreme conditions, often displaying geographic isolation and high degree of endemism. The “phycosphere” refers to the zone around the algae inhabited by microrganisms. Our study used DNA metabarcoding to survey the eukaryotic communities associated with se...
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Academia Brasileira de Ciências
2025-01-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| author | PAULO EDUARDO A.S. CÂMARA FRANCIANE MARIA PELLIZZARI FABYANO A.C. LOPES EDUARDO T. AMORIM FÁBIO L.V. BONES DAFNE A. ANJOS MICHELINE CARVALHO-SILVA PETER CONVEY LUIZ HENRIQUE ROSA |
| author_facet | PAULO EDUARDO A.S. CÂMARA FRANCIANE MARIA PELLIZZARI FABYANO A.C. LOPES EDUARDO T. AMORIM FÁBIO L.V. BONES DAFNE A. ANJOS MICHELINE CARVALHO-SILVA PETER CONVEY LUIZ HENRIQUE ROSA |
| author_sort | PAULO EDUARDO A.S. CÂMARA |
| collection | DOAJ |
| description | Abstract Polar marine macroalgae thrive in extreme conditions, often displaying geographic isolation and high degree of endemism. The “phycosphere” refers to the zone around the algae inhabited by microrganisms. Our study used DNA metabarcoding to survey the eukaryotic communities associated with seven seaweed species obtained at King George Island (South Shetland Islands, maritime Antarctic), including two Rhodophyta, two Chlorophyta and three Phaeophyceae. The ITS2 region was used as a barcode and our analysis yielded 77 eukaryotic ASVs spanning five Kingdoms (Fungi, Metazoa, Chromista, Protozoa, and Viridiplantae) and ten phyla (Ascomycota, Basidiomycota, Cercozoa, Ciliophora, Ochrophyta, Amebozoa, Chlorophyta, Rhodophyta, Bryophyta and Cnidaria). Additionally, we identified 14 potential new occurrence records for Antarctica. Ciliates and green algae were the most species-rich groups. The most abundant assigned associated species was Monostroma angicava (Chrorophyta). Within the macroalgal, the Chlorophyceans Ulothrix sp. hosted the greatest number of taxa, followed by Monostroma hariotii. Our data suggested that Antarctic macroalgae host a rich diversity of associated organisms and the biodiversity associated with the phycosphere remains underestimated. |
| format | Article |
| id | doaj-art-7b94427c1c7d4a78b07134e57e7920d8 |
| institution | Kabale University |
| issn | 1678-2690 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Academia Brasileira de Ciências |
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| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj-art-7b94427c1c7d4a78b07134e57e7920d82025-01-21T07:36:56ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902025-01-0196suppl 210.1590/0001-3765202420240570DNA metabarcoding reveal hidden diversity of periphytic eukaryotes on marine Antarctic macroalgaePAULO EDUARDO A.S. CÂMARAhttps://orcid.org/0000-0002-3944-996XFRANCIANE MARIA PELLIZZARIhttps://orcid.org/0000-0003-1877-2570FABYANO A.C. LOPEShttps://orcid.org/0000-0002-4565-9103EDUARDO T. AMORIMhttps://orcid.org/0000-0003-4253-1339FÁBIO L.V. BONEShttps://orcid.org/0000-0003-2956-1859DAFNE A. ANJOShttps://orcid.org/0000-0002-0769-2279MICHELINE CARVALHO-SILVAhttps://orcid.org/0000-0002-2389-3804PETER CONVEYhttps://orcid.org/0000-0001-8497-9903LUIZ HENRIQUE ROSAhttps://orcid.org/0000-0001-9749-5182Abstract Polar marine macroalgae thrive in extreme conditions, often displaying geographic isolation and high degree of endemism. The “phycosphere” refers to the zone around the algae inhabited by microrganisms. Our study used DNA metabarcoding to survey the eukaryotic communities associated with seven seaweed species obtained at King George Island (South Shetland Islands, maritime Antarctic), including two Rhodophyta, two Chlorophyta and three Phaeophyceae. The ITS2 region was used as a barcode and our analysis yielded 77 eukaryotic ASVs spanning five Kingdoms (Fungi, Metazoa, Chromista, Protozoa, and Viridiplantae) and ten phyla (Ascomycota, Basidiomycota, Cercozoa, Ciliophora, Ochrophyta, Amebozoa, Chlorophyta, Rhodophyta, Bryophyta and Cnidaria). Additionally, we identified 14 potential new occurrence records for Antarctica. Ciliates and green algae were the most species-rich groups. The most abundant assigned associated species was Monostroma angicava (Chrorophyta). Within the macroalgal, the Chlorophyceans Ulothrix sp. hosted the greatest number of taxa, followed by Monostroma hariotii. Our data suggested that Antarctic macroalgae host a rich diversity of associated organisms and the biodiversity associated with the phycosphere remains underestimated.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652024000400904&lng=en&tlng=enchlorophytahigh throughput sequencingKing George IslandPhaeophyceaeRhodophytaseaweeds |
| spellingShingle | PAULO EDUARDO A.S. CÂMARA FRANCIANE MARIA PELLIZZARI FABYANO A.C. LOPES EDUARDO T. AMORIM FÁBIO L.V. BONES DAFNE A. ANJOS MICHELINE CARVALHO-SILVA PETER CONVEY LUIZ HENRIQUE ROSA DNA metabarcoding reveal hidden diversity of periphytic eukaryotes on marine Antarctic macroalgae Anais da Academia Brasileira de Ciências chlorophyta high throughput sequencing King George Island Phaeophyceae Rhodophyta seaweeds |
| title | DNA metabarcoding reveal hidden diversity of periphytic eukaryotes on marine Antarctic macroalgae |
| title_full | DNA metabarcoding reveal hidden diversity of periphytic eukaryotes on marine Antarctic macroalgae |
| title_fullStr | DNA metabarcoding reveal hidden diversity of periphytic eukaryotes on marine Antarctic macroalgae |
| title_full_unstemmed | DNA metabarcoding reveal hidden diversity of periphytic eukaryotes on marine Antarctic macroalgae |
| title_short | DNA metabarcoding reveal hidden diversity of periphytic eukaryotes on marine Antarctic macroalgae |
| title_sort | dna metabarcoding reveal hidden diversity of periphytic eukaryotes on marine antarctic macroalgae |
| topic | chlorophyta high throughput sequencing King George Island Phaeophyceae Rhodophyta seaweeds |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652024000400904&lng=en&tlng=en |
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