Toxic Effects of Ethyl Cinnamate on the Photosynthesis and Physiological Characteristics of Chlorella vulgaris Based on Chlorophyll Fluorescence and Flow Cytometry Analysis
The toxic effects of ethyl cinnamate on the photosynthetic and physiological characteristics of Chlorella vulgaris were studied based on chlorophyll fluorescence and flow cytometry analysis. Parameters, including biomass, Fv/Fm (maximal photochemical efficiency of PSII), ФPSII (actual photochemical...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2015/107823 |
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| author | Yang Jiao Hui-Ling Ouyang Yu-Jiao Jiang Xiang-Zhen Kong Wei He Wen-Xiu Liu Bin Yang Fu-Liu Xu |
| author_facet | Yang Jiao Hui-Ling Ouyang Yu-Jiao Jiang Xiang-Zhen Kong Wei He Wen-Xiu Liu Bin Yang Fu-Liu Xu |
| author_sort | Yang Jiao |
| collection | DOAJ |
| description | The toxic effects of ethyl cinnamate on the photosynthetic and physiological characteristics of Chlorella vulgaris were studied based on chlorophyll fluorescence and flow cytometry analysis. Parameters, including biomass, Fv/Fm (maximal photochemical efficiency of PSII), ФPSII (actual photochemical efficiency of PSII in the light), FDA, and PI staining fluorescence, were measured. The results showed the following: (1) The inhibition on biomass increased as the exposure concentration increased. 1 mg/L ethyl cinnamate was sufficient to reduce the total biomass of C. vulgaris. The 48-h and 72-h EC50 values were 2.07 mg/L (1.94–2.20) and 1.89 mg/L (1.82–1.97). (2) After 24 h of exposure to 2–4 mg/L ethyl cinnamate, the photosynthesis of C. vulgaris almost ceased, manifesting in ФPSII being close to zero. After 72 h of exposure to 4 mg/L ethyl cinnamate, the Fv/Fm of C. vulgaris dropped to zero. (3) Ethyl cinnamate also affected the cellular physiology of C. vulgaris, but these effects resulted in the inhibition of cell yield rather than cell death. Exposure to ethyl cinnamate resulted in decreased esterase activities in C. vulgaris, increased average cell size, and altered intensities of chlorophyll a fluorescence. Overall, esterase activity was the most sensitive variable. |
| format | Article |
| id | doaj-art-7b8289a4879d48b3b26c328c747b4aac |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-7b8289a4879d48b3b26c328c747b4aac2025-02-03T06:12:29ZengWileyThe Scientific World Journal2356-61401537-744X2015-01-01201510.1155/2015/107823107823Toxic Effects of Ethyl Cinnamate on the Photosynthesis and Physiological Characteristics of Chlorella vulgaris Based on Chlorophyll Fluorescence and Flow Cytometry AnalysisYang Jiao0Hui-Ling Ouyang1Yu-Jiao Jiang2Xiang-Zhen Kong3Wei He4Wen-Xiu Liu5Bin Yang6Fu-Liu Xu7MOE Laboratory for Earth Surface Processes, College of Urban & Environmental Sciences, Peking University, Beijing 100871, ChinaMOE Laboratory for Earth Surface Processes, College of Urban & Environmental Sciences, Peking University, Beijing 100871, ChinaMOE Laboratory for Earth Surface Processes, College of Urban & Environmental Sciences, Peking University, Beijing 100871, ChinaMOE Laboratory for Earth Surface Processes, College of Urban & Environmental Sciences, Peking University, Beijing 100871, ChinaMOE Laboratory for Earth Surface Processes, College of Urban & Environmental Sciences, Peking University, Beijing 100871, ChinaMOE Laboratory for Earth Surface Processes, College of Urban & Environmental Sciences, Peking University, Beijing 100871, ChinaMOE Laboratory for Earth Surface Processes, College of Urban & Environmental Sciences, Peking University, Beijing 100871, ChinaMOE Laboratory for Earth Surface Processes, College of Urban & Environmental Sciences, Peking University, Beijing 100871, ChinaThe toxic effects of ethyl cinnamate on the photosynthetic and physiological characteristics of Chlorella vulgaris were studied based on chlorophyll fluorescence and flow cytometry analysis. Parameters, including biomass, Fv/Fm (maximal photochemical efficiency of PSII), ФPSII (actual photochemical efficiency of PSII in the light), FDA, and PI staining fluorescence, were measured. The results showed the following: (1) The inhibition on biomass increased as the exposure concentration increased. 1 mg/L ethyl cinnamate was sufficient to reduce the total biomass of C. vulgaris. The 48-h and 72-h EC50 values were 2.07 mg/L (1.94–2.20) and 1.89 mg/L (1.82–1.97). (2) After 24 h of exposure to 2–4 mg/L ethyl cinnamate, the photosynthesis of C. vulgaris almost ceased, manifesting in ФPSII being close to zero. After 72 h of exposure to 4 mg/L ethyl cinnamate, the Fv/Fm of C. vulgaris dropped to zero. (3) Ethyl cinnamate also affected the cellular physiology of C. vulgaris, but these effects resulted in the inhibition of cell yield rather than cell death. Exposure to ethyl cinnamate resulted in decreased esterase activities in C. vulgaris, increased average cell size, and altered intensities of chlorophyll a fluorescence. Overall, esterase activity was the most sensitive variable.http://dx.doi.org/10.1155/2015/107823 |
| spellingShingle | Yang Jiao Hui-Ling Ouyang Yu-Jiao Jiang Xiang-Zhen Kong Wei He Wen-Xiu Liu Bin Yang Fu-Liu Xu Toxic Effects of Ethyl Cinnamate on the Photosynthesis and Physiological Characteristics of Chlorella vulgaris Based on Chlorophyll Fluorescence and Flow Cytometry Analysis The Scientific World Journal |
| title | Toxic Effects of Ethyl Cinnamate on the Photosynthesis and Physiological Characteristics of Chlorella vulgaris Based on Chlorophyll Fluorescence and Flow Cytometry Analysis |
| title_full | Toxic Effects of Ethyl Cinnamate on the Photosynthesis and Physiological Characteristics of Chlorella vulgaris Based on Chlorophyll Fluorescence and Flow Cytometry Analysis |
| title_fullStr | Toxic Effects of Ethyl Cinnamate on the Photosynthesis and Physiological Characteristics of Chlorella vulgaris Based on Chlorophyll Fluorescence and Flow Cytometry Analysis |
| title_full_unstemmed | Toxic Effects of Ethyl Cinnamate on the Photosynthesis and Physiological Characteristics of Chlorella vulgaris Based on Chlorophyll Fluorescence and Flow Cytometry Analysis |
| title_short | Toxic Effects of Ethyl Cinnamate on the Photosynthesis and Physiological Characteristics of Chlorella vulgaris Based on Chlorophyll Fluorescence and Flow Cytometry Analysis |
| title_sort | toxic effects of ethyl cinnamate on the photosynthesis and physiological characteristics of chlorella vulgaris based on chlorophyll fluorescence and flow cytometry analysis |
| url | http://dx.doi.org/10.1155/2015/107823 |
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