Integration of Metabolomics and Transcriptomics to Reveal the Antitumor Mechanism of <i>Dendrobium officinale</i> Polysaccharide-Based Nanocarriers in Enhancing Photodynamic Immunotherapy in Colorectal Cancer
<b>Background</b>: The mechanism of <i>Dendrobium officinale</i> polysaccharide-based nanocarriers in enhancing photodynamic immunotherapy in colorectal cancer (CRC) remains poorly understood. <b>Methods</b>: The effects of TPA-3BCP-loaded cholesteryl hemisuccinat...
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| author | Shengchang Tao Huan Wang Qiufeng Ji Yushan Yang Gang Wei Ruiming Li Benjie Zhou |
| author_facet | Shengchang Tao Huan Wang Qiufeng Ji Yushan Yang Gang Wei Ruiming Li Benjie Zhou |
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| description | <b>Background</b>: The mechanism of <i>Dendrobium officinale</i> polysaccharide-based nanocarriers in enhancing photodynamic immunotherapy in colorectal cancer (CRC) remains poorly understood. <b>Methods</b>: The effects of TPA-3BCP-loaded cholesteryl hemisuccinate–<i>Dendrobium officinale</i> polysaccharide nanoparticles (DOP@3BCP NPs) and their potential molecular mechanism of action in a tumor-bearing mouse model of CRC were investigated using non-targeted metabolomics and transcriptomics. Meanwhile, a histopathological analysis (H&E staining, Ki67 staining, and TUNEL assay) and a qRT-PCR analysis revealed the antitumor effects of DOP@3BCP NPs with and without light activation. <b>Results</b>: Through metabolomics and transcriptomics analysis, we found an alteration in the metabolome and functional pathways in the examined tumor tissues. The metabolic analysis showed 69 and 60 differentially expressed metabolites (DEMs) in positive- and negative-ion modes, respectively, in the treated samples compared to the Control samples. The transcriptomics analysis showed that 1352 genes were differentially expressed among the three groups. The differentially regulated functional pathways were primally related to the antitumor immune response. The results of the pathological histology assay and qRT-PCR analysis verified the findings of the integrated metabolomics and transcriptomics analysis. <b>Conclusions</b>: Overall, our findings elucidate the potential antitumor mechanisms of the <i>D. officinale</i> polysaccharide-based nanocarrier in enhancing photodynamic immunotherapy in CRC. |
| format | Article |
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| institution | Kabale University |
| issn | 1999-4923 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-7b7b9752a9c34d59a2e92f2c201699f12025-01-24T13:45:56ZengMDPI AGPharmaceutics1999-49232025-01-011719710.3390/pharmaceutics17010097Integration of Metabolomics and Transcriptomics to Reveal the Antitumor Mechanism of <i>Dendrobium officinale</i> Polysaccharide-Based Nanocarriers in Enhancing Photodynamic Immunotherapy in Colorectal CancerShengchang Tao0Huan Wang1Qiufeng Ji2Yushan Yang3Gang Wei4Ruiming Li5Benjie Zhou6Department of Pharmacy, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, ChinaSchool of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaDepartment of Pharmacy, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, ChinaDepartment of Pharmacy, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, ChinaSchool of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaDepartment of Pharmacy, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, ChinaDepartment of Pharmacy, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, China<b>Background</b>: The mechanism of <i>Dendrobium officinale</i> polysaccharide-based nanocarriers in enhancing photodynamic immunotherapy in colorectal cancer (CRC) remains poorly understood. <b>Methods</b>: The effects of TPA-3BCP-loaded cholesteryl hemisuccinate–<i>Dendrobium officinale</i> polysaccharide nanoparticles (DOP@3BCP NPs) and their potential molecular mechanism of action in a tumor-bearing mouse model of CRC were investigated using non-targeted metabolomics and transcriptomics. Meanwhile, a histopathological analysis (H&E staining, Ki67 staining, and TUNEL assay) and a qRT-PCR analysis revealed the antitumor effects of DOP@3BCP NPs with and without light activation. <b>Results</b>: Through metabolomics and transcriptomics analysis, we found an alteration in the metabolome and functional pathways in the examined tumor tissues. The metabolic analysis showed 69 and 60 differentially expressed metabolites (DEMs) in positive- and negative-ion modes, respectively, in the treated samples compared to the Control samples. The transcriptomics analysis showed that 1352 genes were differentially expressed among the three groups. The differentially regulated functional pathways were primally related to the antitumor immune response. The results of the pathological histology assay and qRT-PCR analysis verified the findings of the integrated metabolomics and transcriptomics analysis. <b>Conclusions</b>: Overall, our findings elucidate the potential antitumor mechanisms of the <i>D. officinale</i> polysaccharide-based nanocarrier in enhancing photodynamic immunotherapy in CRC.https://www.mdpi.com/1999-4923/17/1/97<i>Dendrobium officinale</i> polysaccharidephotodynamic therapymetabolomicstranscriptomicsantitumor mechanism |
| spellingShingle | Shengchang Tao Huan Wang Qiufeng Ji Yushan Yang Gang Wei Ruiming Li Benjie Zhou Integration of Metabolomics and Transcriptomics to Reveal the Antitumor Mechanism of <i>Dendrobium officinale</i> Polysaccharide-Based Nanocarriers in Enhancing Photodynamic Immunotherapy in Colorectal Cancer Pharmaceutics <i>Dendrobium officinale</i> polysaccharide photodynamic therapy metabolomics transcriptomics antitumor mechanism |
| title | Integration of Metabolomics and Transcriptomics to Reveal the Antitumor Mechanism of <i>Dendrobium officinale</i> Polysaccharide-Based Nanocarriers in Enhancing Photodynamic Immunotherapy in Colorectal Cancer |
| title_full | Integration of Metabolomics and Transcriptomics to Reveal the Antitumor Mechanism of <i>Dendrobium officinale</i> Polysaccharide-Based Nanocarriers in Enhancing Photodynamic Immunotherapy in Colorectal Cancer |
| title_fullStr | Integration of Metabolomics and Transcriptomics to Reveal the Antitumor Mechanism of <i>Dendrobium officinale</i> Polysaccharide-Based Nanocarriers in Enhancing Photodynamic Immunotherapy in Colorectal Cancer |
| title_full_unstemmed | Integration of Metabolomics and Transcriptomics to Reveal the Antitumor Mechanism of <i>Dendrobium officinale</i> Polysaccharide-Based Nanocarriers in Enhancing Photodynamic Immunotherapy in Colorectal Cancer |
| title_short | Integration of Metabolomics and Transcriptomics to Reveal the Antitumor Mechanism of <i>Dendrobium officinale</i> Polysaccharide-Based Nanocarriers in Enhancing Photodynamic Immunotherapy in Colorectal Cancer |
| title_sort | integration of metabolomics and transcriptomics to reveal the antitumor mechanism of i dendrobium officinale i polysaccharide based nanocarriers in enhancing photodynamic immunotherapy in colorectal cancer |
| topic | <i>Dendrobium officinale</i> polysaccharide photodynamic therapy metabolomics transcriptomics antitumor mechanism |
| url | https://www.mdpi.com/1999-4923/17/1/97 |
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