Molecular Characterization of Chicken Anemia Virus Circulating in Chicken Flocks in Egypt
Introduction. Although many previous studies reported detection of chicken anemia virus (CAV) in Egypt since 1990, genomic characterization of this circulating CAV has not been published. In the present study, four nucleotide sequences of detected CAV were genetically characterized. Methods. These n...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2014/797151 |
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| author | Mohammed AboElkhair Alaa G. Abd El-Razak Abd Elnaby Y. Metwally |
| author_facet | Mohammed AboElkhair Alaa G. Abd El-Razak Abd Elnaby Y. Metwally |
| author_sort | Mohammed AboElkhair |
| collection | DOAJ |
| description | Introduction. Although many previous studies reported detection of chicken anemia virus (CAV) in Egypt since 1990, genomic characterization of this circulating CAV has not been published. In the present study, four nucleotide sequences of detected CAV were genetically characterized. Methods. These nucleotide sequences were obtained from commercial chicken flocks in two different locations of Egypt during 2010. The target region for sequencing was 675 bp nucleotide of partial coding region of VP1 protein. The nucleotide and deduced amino acid sequences of the detected CAV were aligned and compared to worldwide CAV isolates including commonly used vaccine strains. Phylogenetic analysis of these sequences was also carried out. Results. Our results showed that all the Egyptian CAV sequences were grouped in one group with viruses from diverse geographic regions. This group is characterized by amino acids profile 75I, 97L, 139Q, and 144Q in VP1. The phylogenetic and amino acid analyses of deduced amino acid indicated that the detected CAV sequences differ from CAV vaccine strains. Conclusion. This is the first report that describes molecular characterization of circulating CAV in Egypt. The study showed that the detected CAV, in Egypt are field viruses and unrelated to vaccine strains. |
| format | Article |
| id | doaj-art-7b7637a6073044ba942a574deb267641 |
| institution | Kabale University |
| issn | 1687-8639 1687-8647 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-7b7637a6073044ba942a574deb2676412025-02-03T00:58:54ZengWileyAdvances in Virology1687-86391687-86472014-01-01201410.1155/2014/797151797151Molecular Characterization of Chicken Anemia Virus Circulating in Chicken Flocks in EgyptMohammed AboElkhair0Alaa G. Abd El-Razak1Abd Elnaby Y. Metwally2Department of Virology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Minoufiya, EgyptDepartment of Bird and Rabbits Medicine, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Minoufiya, EgyptAnimal Health Research Institute, Kafr El Sheikh Provincial Laboratory, Kafr El Sheikh, EgyptIntroduction. Although many previous studies reported detection of chicken anemia virus (CAV) in Egypt since 1990, genomic characterization of this circulating CAV has not been published. In the present study, four nucleotide sequences of detected CAV were genetically characterized. Methods. These nucleotide sequences were obtained from commercial chicken flocks in two different locations of Egypt during 2010. The target region for sequencing was 675 bp nucleotide of partial coding region of VP1 protein. The nucleotide and deduced amino acid sequences of the detected CAV were aligned and compared to worldwide CAV isolates including commonly used vaccine strains. Phylogenetic analysis of these sequences was also carried out. Results. Our results showed that all the Egyptian CAV sequences were grouped in one group with viruses from diverse geographic regions. This group is characterized by amino acids profile 75I, 97L, 139Q, and 144Q in VP1. The phylogenetic and amino acid analyses of deduced amino acid indicated that the detected CAV sequences differ from CAV vaccine strains. Conclusion. This is the first report that describes molecular characterization of circulating CAV in Egypt. The study showed that the detected CAV, in Egypt are field viruses and unrelated to vaccine strains.http://dx.doi.org/10.1155/2014/797151 |
| spellingShingle | Mohammed AboElkhair Alaa G. Abd El-Razak Abd Elnaby Y. Metwally Molecular Characterization of Chicken Anemia Virus Circulating in Chicken Flocks in Egypt Advances in Virology |
| title | Molecular Characterization of Chicken Anemia Virus Circulating in Chicken Flocks in Egypt |
| title_full | Molecular Characterization of Chicken Anemia Virus Circulating in Chicken Flocks in Egypt |
| title_fullStr | Molecular Characterization of Chicken Anemia Virus Circulating in Chicken Flocks in Egypt |
| title_full_unstemmed | Molecular Characterization of Chicken Anemia Virus Circulating in Chicken Flocks in Egypt |
| title_short | Molecular Characterization of Chicken Anemia Virus Circulating in Chicken Flocks in Egypt |
| title_sort | molecular characterization of chicken anemia virus circulating in chicken flocks in egypt |
| url | http://dx.doi.org/10.1155/2014/797151 |
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