Differences in immune status and fecal SCFA between Indonesian stunted children and children with normal nutritional status.
We recently showed that the gut microbiota composition of stunted children was different from that of children with normal nutritional status. Here, we compared immune status and fecal microbial metabolite concentrations between stunted and normal children, and we correlated macronutrient intake (in...
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| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0254300&type=printable |
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| author | Ingrid S Surono Fasli Jalal Syukrini Bahri Andreas Romulo Pratiwi Dyah Kusumo Erida Manalu Yusnita Koen Venema |
| author_facet | Ingrid S Surono Fasli Jalal Syukrini Bahri Andreas Romulo Pratiwi Dyah Kusumo Erida Manalu Yusnita Koen Venema |
| author_sort | Ingrid S Surono |
| collection | DOAJ |
| description | We recently showed that the gut microbiota composition of stunted children was different from that of children with normal nutritional status. Here, we compared immune status and fecal microbial metabolite concentrations between stunted and normal children, and we correlated macronutrient intake (including energy), metabolites and immune status to microbiota composition. The results show that macronutrient intake was lower in stunted children for all components, but after correction for multiple comparison significant only for energy and fat. Only TGF-β was significantly different between stunted children and children of normal nutritional status after correction for multiple comparisons. TNF-alpha, IL-10, lipopolysaccharide binding protein in serum and secretory IgA in feces were not significantly different. Strikingly, all the individual short-chain and branched-chain fatty acids were higher in fecal samples of stunted children (significant for acetate, valerate and total SCFA). These metabolites correlated with a number of different microbial taxa, but due to extensive cross-feeding between microbes, did not show a specific pattern. However, the energy-loss due to higher excretion in stunted children of these metabolites, which can be used as substrate for the host, is striking. Several microbial taxa also correlated to the intake of macronutrients (including dietary fibre) and energy. Eisenbergiella positively correlated with all macronutrients, while an uncharacterized genus within the Succinivibrionaceae family negatively correlated with all macronutrients. These, and the other correlations observed, may provide indication on how to modulate the gut microbiota of stunted children such that their growth lag can be corrected. Trail registered at https://clinicaltrials.gov/ct2/show/NCT04698759. |
| format | Article |
| id | doaj-art-7b61902ada3548d784279e2f71c46d80 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-7b61902ada3548d784279e2f71c46d802025-01-18T05:31:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01167e025430010.1371/journal.pone.0254300Differences in immune status and fecal SCFA between Indonesian stunted children and children with normal nutritional status.Ingrid S SuronoFasli JalalSyukrini BahriAndreas RomuloPratiwi Dyah KusumoErida ManaluYusnitaKoen VenemaWe recently showed that the gut microbiota composition of stunted children was different from that of children with normal nutritional status. Here, we compared immune status and fecal microbial metabolite concentrations between stunted and normal children, and we correlated macronutrient intake (including energy), metabolites and immune status to microbiota composition. The results show that macronutrient intake was lower in stunted children for all components, but after correction for multiple comparison significant only for energy and fat. Only TGF-β was significantly different between stunted children and children of normal nutritional status after correction for multiple comparisons. TNF-alpha, IL-10, lipopolysaccharide binding protein in serum and secretory IgA in feces were not significantly different. Strikingly, all the individual short-chain and branched-chain fatty acids were higher in fecal samples of stunted children (significant for acetate, valerate and total SCFA). These metabolites correlated with a number of different microbial taxa, but due to extensive cross-feeding between microbes, did not show a specific pattern. However, the energy-loss due to higher excretion in stunted children of these metabolites, which can be used as substrate for the host, is striking. Several microbial taxa also correlated to the intake of macronutrients (including dietary fibre) and energy. Eisenbergiella positively correlated with all macronutrients, while an uncharacterized genus within the Succinivibrionaceae family negatively correlated with all macronutrients. These, and the other correlations observed, may provide indication on how to modulate the gut microbiota of stunted children such that their growth lag can be corrected. Trail registered at https://clinicaltrials.gov/ct2/show/NCT04698759.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0254300&type=printable |
| spellingShingle | Ingrid S Surono Fasli Jalal Syukrini Bahri Andreas Romulo Pratiwi Dyah Kusumo Erida Manalu Yusnita Koen Venema Differences in immune status and fecal SCFA between Indonesian stunted children and children with normal nutritional status. PLoS ONE |
| title | Differences in immune status and fecal SCFA between Indonesian stunted children and children with normal nutritional status. |
| title_full | Differences in immune status and fecal SCFA between Indonesian stunted children and children with normal nutritional status. |
| title_fullStr | Differences in immune status and fecal SCFA between Indonesian stunted children and children with normal nutritional status. |
| title_full_unstemmed | Differences in immune status and fecal SCFA between Indonesian stunted children and children with normal nutritional status. |
| title_short | Differences in immune status and fecal SCFA between Indonesian stunted children and children with normal nutritional status. |
| title_sort | differences in immune status and fecal scfa between indonesian stunted children and children with normal nutritional status |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0254300&type=printable |
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