Clinical Comparison of QUANTA Flash dsDNA Chemiluminescent Immunoassay with Four Current Assays for the Detection of Anti-dsDNA Autoantibodies
Introduction. The objective of the present study was to compare QUANTA Flash dsDNA, a chemiluminescent immunoassay (CIA) on the BIO-FLASH, a rapid-response chemiluminescent analyzer, to three other anti-dsDNA antibody assays and to Crithidia luciliae indirect immunofluorescence test (CLIFT). Methods...
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| Format: | Article |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/902821 |
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| author | Maria Infantino Francesca Meacci Chelsea Bentow Peter Martis Maurizio Benucci Antonella Afeltra Amelia Rigon Fabiola Atzeni Piercarlo Sarzi-Puttini Mariangela Manfredi Michael Mahler |
| author_facet | Maria Infantino Francesca Meacci Chelsea Bentow Peter Martis Maurizio Benucci Antonella Afeltra Amelia Rigon Fabiola Atzeni Piercarlo Sarzi-Puttini Mariangela Manfredi Michael Mahler |
| author_sort | Maria Infantino |
| collection | DOAJ |
| description | Introduction. The objective of the present study was to compare QUANTA Flash dsDNA, a chemiluminescent immunoassay (CIA) on the BIO-FLASH, a rapid-response chemiluminescent analyzer, to three other anti-dsDNA antibody assays and to Crithidia luciliae indirect immunofluorescence test (CLIFT). Methods. In the first part of the study, 161 samples, 61 from patients suffering from systemic lupus erythematosus (SLE) and 100 from a disease control group, were tested by QUANTA Flash dsDNA CIA, QUANTA Lite dsDNA SC ELISA, BioPlex 2200 multiplex flow immunoassay (MFI), ImmuLisa dsDNA ELISA, and NOVA Lite CLIFT. A second cohort of 69 SLE patients was then tested by QUANTA Flash dsDNA and CLIFT to expand the study. Results. The overall qualitative agreements varied between 77.0% (NOVA Lite CLIFT versus QUANTA Lite) and 89.4% (ImmuLisa versus NOVA Lite CLIFT). The clinical sensitivities for the anti-dsDNA antibody tests varied from 8.2% (NOVA Lite CLIFT) to 54.1% (QUANTA Lite), while the clinical specificities varied from 88.0% (BioPlex 2200) to 100.0% (NOVA Lite CLIFT). Good correlation was found between QUANTA Flash dsDNA and NOVA Lite CLIFT. Conclusion. Significant variations among dsDNA methods were observed. QUANTA Flash dsDNA provides a good combination of sensitivity and specificity for the diagnosis of SLE and good agreement to CLIFT. |
| format | Article |
| id | doaj-art-7b5375647f7843ed81f8f8c3bc45dd4b |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-7b5375647f7843ed81f8f8c3bc45dd4b2025-02-03T05:45:05ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/902821902821Clinical Comparison of QUANTA Flash dsDNA Chemiluminescent Immunoassay with Four Current Assays for the Detection of Anti-dsDNA AutoantibodiesMaria Infantino0Francesca Meacci1Chelsea Bentow2Peter Martis3Maurizio Benucci4Antonella Afeltra5Amelia Rigon6Fabiola Atzeni7Piercarlo Sarzi-Puttini8Mariangela Manfredi9Michael Mahler10Immunology and Allergology Laboratory Unit, S. Giovanni di Dio Hospital, Via Torregalli 3, 50143 Florence, ItalyImmunology and Allergology Laboratory Unit, S. Giovanni di Dio Hospital, Via Torregalli 3, 50143 Florence, ItalyInova Diagnostics, Inc., San Diego, CA 92131, USAInova Diagnostics, Inc., San Diego, CA 92131, USAUO Reumatologia, Ospedale S. Giovanni di Dio, 3055 69321 Firenze, ItalyUOC Medicina Clinica e Reumatologia, Università Campus Bio-Medico, Via Álvaro del Portillo 21, Roma, ItalyUOC Medicina Clinica e Reumatologia, Università Campus Bio-Medico, Via Álvaro del Portillo 21, Roma, ItalyUOC Reumatologia, Ospedale L. Sacco Polo Universitario, Via Giovanni Battista Grassi 74, 20157 Milano, ItalyUOC Reumatologia, Ospedale L. Sacco Polo Universitario, Via Giovanni Battista Grassi 74, 20157 Milano, ItalyImmunology and Allergology Laboratory Unit, S. Giovanni di Dio Hospital, Via Torregalli 3, 50143 Florence, ItalyInova Diagnostics, Inc., San Diego, CA 92131, USAIntroduction. The objective of the present study was to compare QUANTA Flash dsDNA, a chemiluminescent immunoassay (CIA) on the BIO-FLASH, a rapid-response chemiluminescent analyzer, to three other anti-dsDNA antibody assays and to Crithidia luciliae indirect immunofluorescence test (CLIFT). Methods. In the first part of the study, 161 samples, 61 from patients suffering from systemic lupus erythematosus (SLE) and 100 from a disease control group, were tested by QUANTA Flash dsDNA CIA, QUANTA Lite dsDNA SC ELISA, BioPlex 2200 multiplex flow immunoassay (MFI), ImmuLisa dsDNA ELISA, and NOVA Lite CLIFT. A second cohort of 69 SLE patients was then tested by QUANTA Flash dsDNA and CLIFT to expand the study. Results. The overall qualitative agreements varied between 77.0% (NOVA Lite CLIFT versus QUANTA Lite) and 89.4% (ImmuLisa versus NOVA Lite CLIFT). The clinical sensitivities for the anti-dsDNA antibody tests varied from 8.2% (NOVA Lite CLIFT) to 54.1% (QUANTA Lite), while the clinical specificities varied from 88.0% (BioPlex 2200) to 100.0% (NOVA Lite CLIFT). Good correlation was found between QUANTA Flash dsDNA and NOVA Lite CLIFT. Conclusion. Significant variations among dsDNA methods were observed. QUANTA Flash dsDNA provides a good combination of sensitivity and specificity for the diagnosis of SLE and good agreement to CLIFT.http://dx.doi.org/10.1155/2015/902821 |
| spellingShingle | Maria Infantino Francesca Meacci Chelsea Bentow Peter Martis Maurizio Benucci Antonella Afeltra Amelia Rigon Fabiola Atzeni Piercarlo Sarzi-Puttini Mariangela Manfredi Michael Mahler Clinical Comparison of QUANTA Flash dsDNA Chemiluminescent Immunoassay with Four Current Assays for the Detection of Anti-dsDNA Autoantibodies Journal of Immunology Research |
| title | Clinical Comparison of QUANTA Flash dsDNA Chemiluminescent Immunoassay with Four Current Assays for the Detection of Anti-dsDNA Autoantibodies |
| title_full | Clinical Comparison of QUANTA Flash dsDNA Chemiluminescent Immunoassay with Four Current Assays for the Detection of Anti-dsDNA Autoantibodies |
| title_fullStr | Clinical Comparison of QUANTA Flash dsDNA Chemiluminescent Immunoassay with Four Current Assays for the Detection of Anti-dsDNA Autoantibodies |
| title_full_unstemmed | Clinical Comparison of QUANTA Flash dsDNA Chemiluminescent Immunoassay with Four Current Assays for the Detection of Anti-dsDNA Autoantibodies |
| title_short | Clinical Comparison of QUANTA Flash dsDNA Chemiluminescent Immunoassay with Four Current Assays for the Detection of Anti-dsDNA Autoantibodies |
| title_sort | clinical comparison of quanta flash dsdna chemiluminescent immunoassay with four current assays for the detection of anti dsdna autoantibodies |
| url | http://dx.doi.org/10.1155/2015/902821 |
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