Investigational Management for a Positive NIPT Result - Case Report

Non-invasive prenatal testing (NIPT), since its introduction in 2011, has revolutionized prenatal screening, becoming widely used globally and replacing traditional screening methods in developed countries. The accuracy of NIPT in detecting aneuploidies is extremely high and there has been a recent...

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Main Authors: Elena Evelina STOICA, Laurentiu Camil BOHILTEA, Delia-Maria Gradinaru-FOMETESCU, Monica Mihaela CIRSTOIU
Format: Article
Language:English
Published: Bucharest College of Physicians 2024-06-01
Series:Modern Medicine
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Online Access:https://medicinamoderna.ro/wp-content/uploads/2024/06/Investigational-Management-for-a-Positive-NIPT-Result-Case-Report.pdf
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author Elena Evelina STOICA
Laurentiu Camil BOHILTEA
Delia-Maria Gradinaru-FOMETESCU
Monica Mihaela CIRSTOIU
author_facet Elena Evelina STOICA
Laurentiu Camil BOHILTEA
Delia-Maria Gradinaru-FOMETESCU
Monica Mihaela CIRSTOIU
author_sort Elena Evelina STOICA
collection DOAJ
description Non-invasive prenatal testing (NIPT), since its introduction in 2011, has revolutionized prenatal screening, becoming widely used globally and replacing traditional screening methods in developed countries. The accuracy of NIPT in detecting aneuploidies is extremely high and there has been a recent trend towards improving NIPT for detecting microdeletion and microduplication syndromes, monogenic diseases, fetal sex determination, and RH genotyping. Significant progress has been made in molecular analysis techniques of fetal DNA, including methods such as massively parallel sequencing, RNA-based testing, digital PCR, and single nucleotide polymorphism analysis. We present the case of a 32-year-old patient who, at 12 weeks of gestation, had a non-invasive prenatal test result showing a maternal 22q11.2 deletion. Following genetic consultation, further investigations were conducted to stratify the fetal risk of inheriting the microdeletion syndrome. As a result, microarray CGH cytoarray from amniotic fluid was performed, and no 22q11.2 deletion was detected. In this case, complete elucidation of the origin of the deletion found in NIPT could not be achieved, as it would require arrayCGH testing for the mother, a test that was not performed due to financial reasons. Given the high rate of genetic syndromes with potential impact on fetal development and familial psychological impact, we wish to emphasize the necessity of financial support from the state to introduce non-invasive prenatal testing into the list of reimbursed analyses covered by health insurance. This would enable superior testing and, implicitly, genetic prevention of all pregnancies, facilitating appropriate risk stratification of pregnancies in our country.
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spelling doaj-art-7b4455f8b9084622a5c6775a0dd23eee2025-08-20T01:58:05ZengBucharest College of PhysiciansModern Medicine1223-04722360-24732024-06-01312187190https://doi.org/10.31689/rmm.2024.31.2.187Investigational Management for a Positive NIPT Result - Case ReportElena Evelina STOICA0Laurentiu Camil BOHILTEA1https://orcid.org/0009-0006-3000-1532Delia-Maria Gradinaru-FOMETESCU2Monica Mihaela CIRSTOIU3University Emergency Hospital Bucharest, Bucharest, Romania“Carol Davila” University of Medicine and Pharmacy, Bucharest, RomaniaUniversity Emergency Hospital Bucharest, Bucharest, Romania“Carol Davila” University of Medicine and Pharmacy, Bucharest, RomaniaNon-invasive prenatal testing (NIPT), since its introduction in 2011, has revolutionized prenatal screening, becoming widely used globally and replacing traditional screening methods in developed countries. The accuracy of NIPT in detecting aneuploidies is extremely high and there has been a recent trend towards improving NIPT for detecting microdeletion and microduplication syndromes, monogenic diseases, fetal sex determination, and RH genotyping. Significant progress has been made in molecular analysis techniques of fetal DNA, including methods such as massively parallel sequencing, RNA-based testing, digital PCR, and single nucleotide polymorphism analysis. We present the case of a 32-year-old patient who, at 12 weeks of gestation, had a non-invasive prenatal test result showing a maternal 22q11.2 deletion. Following genetic consultation, further investigations were conducted to stratify the fetal risk of inheriting the microdeletion syndrome. As a result, microarray CGH cytoarray from amniotic fluid was performed, and no 22q11.2 deletion was detected. In this case, complete elucidation of the origin of the deletion found in NIPT could not be achieved, as it would require arrayCGH testing for the mother, a test that was not performed due to financial reasons. Given the high rate of genetic syndromes with potential impact on fetal development and familial psychological impact, we wish to emphasize the necessity of financial support from the state to introduce non-invasive prenatal testing into the list of reimbursed analyses covered by health insurance. This would enable superior testing and, implicitly, genetic prevention of all pregnancies, facilitating appropriate risk stratification of pregnancies in our country.https://medicinamoderna.ro/wp-content/uploads/2024/06/Investigational-Management-for-a-Positive-NIPT-Result-Case-Report.pdfnon-invasive prenatal testingmicrodeletiondigeorge syndromeaneuploidiesfetal fraction
spellingShingle Elena Evelina STOICA
Laurentiu Camil BOHILTEA
Delia-Maria Gradinaru-FOMETESCU
Monica Mihaela CIRSTOIU
Investigational Management for a Positive NIPT Result - Case Report
Modern Medicine
non-invasive prenatal testing
microdeletion
digeorge syndrome
aneuploidies
fetal fraction
title Investigational Management for a Positive NIPT Result - Case Report
title_full Investigational Management for a Positive NIPT Result - Case Report
title_fullStr Investigational Management for a Positive NIPT Result - Case Report
title_full_unstemmed Investigational Management for a Positive NIPT Result - Case Report
title_short Investigational Management for a Positive NIPT Result - Case Report
title_sort investigational management for a positive nipt result case report
topic non-invasive prenatal testing
microdeletion
digeorge syndrome
aneuploidies
fetal fraction
url https://medicinamoderna.ro/wp-content/uploads/2024/06/Investigational-Management-for-a-Positive-NIPT-Result-Case-Report.pdf
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AT deliamariagradinarufometescu investigationalmanagementforapositiveniptresultcasereport
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