Correlation of Chimerism with Acute Graft-versus-Host Disease in Rats following Liver Transplantation
The accurate diagnosis of acute graft-versus-host disease following liver transplantation (LTx-aGVHD) has been hampered. Chimerism appears in the majority of recipients after LT and its significance in the diagnosis of LTx-aGVHD has not been clearly established. To demonstrate the significance of ch...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | International Journal of Hepatology |
| Online Access: | http://dx.doi.org/10.4061/2011/947150 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832545517396033536 |
|---|---|
| author | Fei Xue Wei Chen Xue-Li Bai Guo-Dong Xu Liang Liang Ting-Bo Liang |
| author_facet | Fei Xue Wei Chen Xue-Li Bai Guo-Dong Xu Liang Liang Ting-Bo Liang |
| author_sort | Fei Xue |
| collection | DOAJ |
| description | The accurate diagnosis of acute graft-versus-host disease following liver transplantation (LTx-aGVHD) has been hampered. Chimerism appears in the majority of recipients after LT and its significance in the diagnosis of LTx-aGVHD has not been clearly established. To demonstrate the significance of chimerism on the diagnosis of LTx-aGVHD, we compared the change of chimerism in syngeneic LT recipients, semiallogeneic LT recipients, and LTx-aGVHD induced recipients. Chimerism in PBMCs following sex-mismatched LT was identified by real-time PCR based on a rat Y-chromosome-specific primer. All recipients in semiallogeneic group grew in a normal pattern. However, when 4×108 donor splenocytes were transferred simultaneously during LT, the morbidity of lethal aGVHD was 100%. The chimerism appeared slightly higher in the semiallogeneic group than in the syngeneic LT group, but the difference was not significant. However, when the recipients developed lethal aGVHD after LT, chimerism in the PBMCs increased progressively, and even at an early time, a significant increase in chimerism was observed. In conclusion, high level chimerism correlated well with LTx-aGVHD, and detection of chimerism soon after transplantation may be of value in the diagnosis of LTx-aGVHD prior to the onset of symptoms. |
| format | Article |
| id | doaj-art-7b34c34c4b3148228a519780c71fa1e1 |
| institution | Kabale University |
| issn | 2090-3448 2090-3456 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Hepatology |
| spelling | doaj-art-7b34c34c4b3148228a519780c71fa1e12025-02-03T07:25:30ZengWileyInternational Journal of Hepatology2090-34482090-34562011-01-01201110.4061/2011/947150947150Correlation of Chimerism with Acute Graft-versus-Host Disease in Rats following Liver TransplantationFei Xue0Wei Chen1Xue-Li Bai2Guo-Dong Xu3Liang Liang4Ting-Bo Liang5Department of Hepatobiliary and Pancreatic Surgery, Key Laboratory of Combined Multi-Organ Transplantation of Ministry of Public Health, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, Key Laboratory of Combined Multi-Organ Transplantation of Ministry of Public Health, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, Key Laboratory of Combined Multi-Organ Transplantation of Ministry of Public Health, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, Key Laboratory of Combined Multi-Organ Transplantation of Ministry of Public Health, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, Key Laboratory of Combined Multi-Organ Transplantation of Ministry of Public Health, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, Key Laboratory of Combined Multi-Organ Transplantation of Ministry of Public Health, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, ChinaThe accurate diagnosis of acute graft-versus-host disease following liver transplantation (LTx-aGVHD) has been hampered. Chimerism appears in the majority of recipients after LT and its significance in the diagnosis of LTx-aGVHD has not been clearly established. To demonstrate the significance of chimerism on the diagnosis of LTx-aGVHD, we compared the change of chimerism in syngeneic LT recipients, semiallogeneic LT recipients, and LTx-aGVHD induced recipients. Chimerism in PBMCs following sex-mismatched LT was identified by real-time PCR based on a rat Y-chromosome-specific primer. All recipients in semiallogeneic group grew in a normal pattern. However, when 4×108 donor splenocytes were transferred simultaneously during LT, the morbidity of lethal aGVHD was 100%. The chimerism appeared slightly higher in the semiallogeneic group than in the syngeneic LT group, but the difference was not significant. However, when the recipients developed lethal aGVHD after LT, chimerism in the PBMCs increased progressively, and even at an early time, a significant increase in chimerism was observed. In conclusion, high level chimerism correlated well with LTx-aGVHD, and detection of chimerism soon after transplantation may be of value in the diagnosis of LTx-aGVHD prior to the onset of symptoms.http://dx.doi.org/10.4061/2011/947150 |
| spellingShingle | Fei Xue Wei Chen Xue-Li Bai Guo-Dong Xu Liang Liang Ting-Bo Liang Correlation of Chimerism with Acute Graft-versus-Host Disease in Rats following Liver Transplantation International Journal of Hepatology |
| title | Correlation of Chimerism with Acute Graft-versus-Host Disease in Rats following Liver Transplantation |
| title_full | Correlation of Chimerism with Acute Graft-versus-Host Disease in Rats following Liver Transplantation |
| title_fullStr | Correlation of Chimerism with Acute Graft-versus-Host Disease in Rats following Liver Transplantation |
| title_full_unstemmed | Correlation of Chimerism with Acute Graft-versus-Host Disease in Rats following Liver Transplantation |
| title_short | Correlation of Chimerism with Acute Graft-versus-Host Disease in Rats following Liver Transplantation |
| title_sort | correlation of chimerism with acute graft versus host disease in rats following liver transplantation |
| url | http://dx.doi.org/10.4061/2011/947150 |
| work_keys_str_mv | AT feixue correlationofchimerismwithacutegraftversushostdiseaseinratsfollowinglivertransplantation AT weichen correlationofchimerismwithacutegraftversushostdiseaseinratsfollowinglivertransplantation AT xuelibai correlationofchimerismwithacutegraftversushostdiseaseinratsfollowinglivertransplantation AT guodongxu correlationofchimerismwithacutegraftversushostdiseaseinratsfollowinglivertransplantation AT liangliang correlationofchimerismwithacutegraftversushostdiseaseinratsfollowinglivertransplantation AT tingboliang correlationofchimerismwithacutegraftversushostdiseaseinratsfollowinglivertransplantation |