Autologous Enhancement by Interferon of Natural Killer Activity of Human Peripheral Blood Lymphocytes
The in vitro action of interferon (IFN)-α and IFN-γ from six healthy donors and ten patients with multiple sclerosis (MS) on natural killer (INK) activity of peripheral blood lymphocytes (PBL) was studied in an autologous system. The NK activity of PBL was detected by a cytotoxic test using 3H-uridi...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
1994-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935194000475 |
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| Summary: | The in vitro action of interferon (IFN)-α and
IFN-γ from six healthy donors and ten patients with multiple
sclerosis (MS) on natural killer (INK) activity of peripheral blood
lymphocytes (PBL) was studied in an autologous system. The NK
activity of PBL was detected by a cytotoxic test using
3H-uridine human erythromyeloblast K562 cells. Autologous
IFN-α and IFN-γ did not augment NK activity of PBL from
healthy donors in vitro, whereas in samples from MS
patients the IFNs strongly stimulated NK cell cytotoxic function.
This stimulation suggests the existence of an inhibitor of
regulatory IFN action, that is produced in healthy donors
simultaneously with IFN in response to IFN induction, but which is
lacking in commercial IFN preparations. The factor-containing
supernatants from healthy donors reduced the stimulatory action of
autologous IFNs in patients with MS almost until complete blockade.
Because this inhibitor was absent in patients with MS, deficiency of
an inhibitor of IFN regulatory action in MS could open the way to
treatment of this compartment of the immune system. |
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| ISSN: | 0962-9351 1466-1861 |