Chaetoceros muelleri sulfated polysaccharides: chain conformation, physical characteristics, and morphology
Increasing interest in biopolymers moves their knowledge frontiers. One area undergoing this development is polysaccharides. The practical and theoretical significance of studying polysaccharides lies in the numerous essential functions these macromolecules fulfill in living organisms, and the impor...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
AIMS Press
2024-11-01
|
| Series: | AIMS Biophysics |
| Subjects: | |
| Online Access: | https://www.aimspress.com/article/doi/10.3934/biophy.2024025 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832590264459329536 |
|---|---|
| author | Valeria Miranda-Arizmendi Jorge Marquez-Escalante Agustin Rascon-Chu Karla Martínez-Robinson Francisco Brown-Bojorquez Elizabeth Carvajal-Millan |
| author_facet | Valeria Miranda-Arizmendi Jorge Marquez-Escalante Agustin Rascon-Chu Karla Martínez-Robinson Francisco Brown-Bojorquez Elizabeth Carvajal-Millan |
| author_sort | Valeria Miranda-Arizmendi |
| collection | DOAJ |
| description | Increasing interest in biopolymers moves their knowledge frontiers. One area undergoing this development is polysaccharides. The practical and theoretical significance of studying polysaccharides lies in the numerous essential functions these macromolecules fulfill in living organisms, and the important industrial and technological purposes they serve. Polysaccharides are thought to be abundant in marine microalgae; nevertheless, little is known about their sulfated polysaccharides. We studied Chaetoceros muelleri sulfated polysaccharide (CMSP) chain conformation, physical characteristics, and morphology. The CMSP spectrum generated from Fourier-transform infrared analysis displayed distinctive bands for these macromolecules. S=O and C–O–S signals were detected at 1225 and 820 cm−1, respectively, confirming the presence of sulfate in the molecules. The biopolymer registered weight-average molar mass, intrinsic viscosity ([η]), radius of gyration (RG), hydrodynamic radius (Rh), and sulfate degree of substitution of 1933 kDa, 577 mL/g, 62 nm, 44 nm, and 0.5 (sulfates per disaccharide repeat unit), respectively. The exponent α and the coefficient K in the Mark-Houwink-Sakurada (MHS) equation were 0.76 and 9.76 x 10−3 mL/g, respectively. These values suggest a flexible and compact random coil structure in CMSP. The sample's zeta potential (ζ), conductivity, and diffusion coefficient (D) were −26.43 mV, −2.07 µm cm/s V, 1.25 mS/cm, and 1.8 × 10−8 cm2/s, correspondingly. The negative charge in the molecules is related to the sulfate groups contained. The CMSP surface was coarse and craggy, according to scanning electron microscopy (SEM). The information generated in this present study contributes to elucidating characteristics as fundamental knowledge to understand the macromolecule functionality. |
| format | Article |
| id | doaj-art-7ae519ce95b34e23be6abbfc462a2715 |
| institution | Kabale University |
| issn | 2377-9098 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | AIMS Biophysics |
| spelling | doaj-art-7ae519ce95b34e23be6abbfc462a27152025-01-24T01:20:10ZengAIMS PressAIMS Biophysics2377-90982024-11-0111445546310.3934/biophy.2024025Chaetoceros muelleri sulfated polysaccharides: chain conformation, physical characteristics, and morphologyValeria Miranda-Arizmendi0Jorge Marquez-Escalante1Agustin Rascon-Chu2Karla Martínez-Robinson3Francisco Brown-Bojorquez4Elizabeth Carvajal-Millan5Research Center for Food and Development (CIAD, AC), Carretera Gustavo Enrique Astiazarán Rosas No. 46, Col. La Victoria, 83304 Hermosillo, Sonora, MexicoResearch Center for Food and Development (CIAD, AC), Carretera Gustavo Enrique Astiazarán Rosas No. 46, Col. La Victoria, 83304 Hermosillo, Sonora, MexicoResearch Center for Food and Development (CIAD, AC), Carretera Gustavo Enrique Astiazarán Rosas No. 46, Col. La Victoria, 83304 Hermosillo, Sonora, MexicoResearch Center for Food and Development (CIAD, AC), Carretera Gustavo Enrique Astiazarán Rosas No. 46, Col. La Victoria, 83304 Hermosillo, Sonora, MexicoDepartment of Polymers, University of Sonora, Blvd. Luis Donaldo Colosio, S/N, Hermosillo 83000, Sonora, MexicoResearch Center for Food and Development (CIAD, AC), Carretera Gustavo Enrique Astiazarán Rosas No. 46, Col. La Victoria, 83304 Hermosillo, Sonora, MexicoIncreasing interest in biopolymers moves their knowledge frontiers. One area undergoing this development is polysaccharides. The practical and theoretical significance of studying polysaccharides lies in the numerous essential functions these macromolecules fulfill in living organisms, and the important industrial and technological purposes they serve. Polysaccharides are thought to be abundant in marine microalgae; nevertheless, little is known about their sulfated polysaccharides. We studied Chaetoceros muelleri sulfated polysaccharide (CMSP) chain conformation, physical characteristics, and morphology. The CMSP spectrum generated from Fourier-transform infrared analysis displayed distinctive bands for these macromolecules. S=O and C–O–S signals were detected at 1225 and 820 cm−1, respectively, confirming the presence of sulfate in the molecules. The biopolymer registered weight-average molar mass, intrinsic viscosity ([η]), radius of gyration (RG), hydrodynamic radius (Rh), and sulfate degree of substitution of 1933 kDa, 577 mL/g, 62 nm, 44 nm, and 0.5 (sulfates per disaccharide repeat unit), respectively. The exponent α and the coefficient K in the Mark-Houwink-Sakurada (MHS) equation were 0.76 and 9.76 x 10−3 mL/g, respectively. These values suggest a flexible and compact random coil structure in CMSP. The sample's zeta potential (ζ), conductivity, and diffusion coefficient (D) were −26.43 mV, −2.07 µm cm/s V, 1.25 mS/cm, and 1.8 × 10−8 cm2/s, correspondingly. The negative charge in the molecules is related to the sulfate groups contained. The CMSP surface was coarse and craggy, according to scanning electron microscopy (SEM). The information generated in this present study contributes to elucidating characteristics as fundamental knowledge to understand the macromolecule functionality.https://www.aimspress.com/article/doi/10.3934/biophy.2024025microalga polysaccharidesmacromolecular characterizationmulti-angle light scatteringscanning electron microscopy |
| spellingShingle | Valeria Miranda-Arizmendi Jorge Marquez-Escalante Agustin Rascon-Chu Karla Martínez-Robinson Francisco Brown-Bojorquez Elizabeth Carvajal-Millan Chaetoceros muelleri sulfated polysaccharides: chain conformation, physical characteristics, and morphology AIMS Biophysics microalga polysaccharides macromolecular characterization multi-angle light scattering scanning electron microscopy |
| title | Chaetoceros muelleri sulfated polysaccharides: chain conformation, physical characteristics, and morphology |
| title_full | Chaetoceros muelleri sulfated polysaccharides: chain conformation, physical characteristics, and morphology |
| title_fullStr | Chaetoceros muelleri sulfated polysaccharides: chain conformation, physical characteristics, and morphology |
| title_full_unstemmed | Chaetoceros muelleri sulfated polysaccharides: chain conformation, physical characteristics, and morphology |
| title_short | Chaetoceros muelleri sulfated polysaccharides: chain conformation, physical characteristics, and morphology |
| title_sort | chaetoceros muelleri sulfated polysaccharides chain conformation physical characteristics and morphology |
| topic | microalga polysaccharides macromolecular characterization multi-angle light scattering scanning electron microscopy |
| url | https://www.aimspress.com/article/doi/10.3934/biophy.2024025 |
| work_keys_str_mv | AT valeriamirandaarizmendi chaetocerosmuellerisulfatedpolysaccharideschainconformationphysicalcharacteristicsandmorphology AT jorgemarquezescalante chaetocerosmuellerisulfatedpolysaccharideschainconformationphysicalcharacteristicsandmorphology AT agustinrasconchu chaetocerosmuellerisulfatedpolysaccharideschainconformationphysicalcharacteristicsandmorphology AT karlamartinezrobinson chaetocerosmuellerisulfatedpolysaccharideschainconformationphysicalcharacteristicsandmorphology AT franciscobrownbojorquez chaetocerosmuellerisulfatedpolysaccharideschainconformationphysicalcharacteristicsandmorphology AT elizabethcarvajalmillan chaetocerosmuellerisulfatedpolysaccharideschainconformationphysicalcharacteristicsandmorphology |