Alpha-Lipoic Acid Attenuates Cerebral Ischemia and Reperfusion Injury via Insulin Receptor and PI3K/Akt-Dependent Inhibition of NADPH Oxidase
Alpha-lipoic acid (ALA) has various pharmacological effects such as antioxidative, anti-inflammatory, and antiapoptotic properties. In the present study, administration of ALA (40 mg/kg, i.p.) for 3 days resulted in a significant decrease in neuronal deficit score and infarct volume and a significan...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2015/903186 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558185707208704 |
|---|---|
| author | Yinhua Dong Hongxin Wang Zefeng Chen |
| author_facet | Yinhua Dong Hongxin Wang Zefeng Chen |
| author_sort | Yinhua Dong |
| collection | DOAJ |
| description | Alpha-lipoic acid (ALA) has various pharmacological effects such as antioxidative, anti-inflammatory, and antiapoptotic properties. In the present study, administration of ALA (40 mg/kg, i.p.) for 3 days resulted in a significant decrease in neuronal deficit score and infarct volume and a significant increase in grip time and latency time in Morris water maze at 48 h after middle cerebral artery occlusion and reperfusion (MCAO/R) in rats. ALA also reduced the increased TUNEL-positive cells rate and the enhanced caspase-3 activity induced by MCAO/R. However, the underlying mechanisms remain poorly understood. In this study, we found that ALA could activate insulin receptor and PI3K/Akt signaling pathways, inhibit the expression and activity of NADPH oxidase, and subsequently suppress the generation of superoxide and the augment of oxidative stress indicators including MDA, protein carbonylation, and 8-OHdG. In conclusion, ALA attenuates cerebral ischemia and reperfusion injury via insulin receptor and PI3K/Akt-dependent inhibition of NADPH oxidase. |
| format | Article |
| id | doaj-art-7adc3c47dfd9489f979e844d32137134 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-7adc3c47dfd9489f979e844d321371342025-02-03T01:32:56ZengWileyInternational Journal of Endocrinology1687-83371687-83452015-01-01201510.1155/2015/903186903186Alpha-Lipoic Acid Attenuates Cerebral Ischemia and Reperfusion Injury via Insulin Receptor and PI3K/Akt-Dependent Inhibition of NADPH OxidaseYinhua Dong0Hongxin Wang1Zefeng Chen2Department of Neurology, The Affiliated Fourth Centre Hospital of Tianjin Medical University, Tianjin 300140, ChinaDepartment of Neurology, The Affiliated Fourth Centre Hospital of Tianjin Medical University, Tianjin 300140, ChinaDepartment of Neurology, The Affiliated Fourth Centre Hospital of Tianjin Medical University, Tianjin 300140, ChinaAlpha-lipoic acid (ALA) has various pharmacological effects such as antioxidative, anti-inflammatory, and antiapoptotic properties. In the present study, administration of ALA (40 mg/kg, i.p.) for 3 days resulted in a significant decrease in neuronal deficit score and infarct volume and a significant increase in grip time and latency time in Morris water maze at 48 h after middle cerebral artery occlusion and reperfusion (MCAO/R) in rats. ALA also reduced the increased TUNEL-positive cells rate and the enhanced caspase-3 activity induced by MCAO/R. However, the underlying mechanisms remain poorly understood. In this study, we found that ALA could activate insulin receptor and PI3K/Akt signaling pathways, inhibit the expression and activity of NADPH oxidase, and subsequently suppress the generation of superoxide and the augment of oxidative stress indicators including MDA, protein carbonylation, and 8-OHdG. In conclusion, ALA attenuates cerebral ischemia and reperfusion injury via insulin receptor and PI3K/Akt-dependent inhibition of NADPH oxidase.http://dx.doi.org/10.1155/2015/903186 |
| spellingShingle | Yinhua Dong Hongxin Wang Zefeng Chen Alpha-Lipoic Acid Attenuates Cerebral Ischemia and Reperfusion Injury via Insulin Receptor and PI3K/Akt-Dependent Inhibition of NADPH Oxidase International Journal of Endocrinology |
| title | Alpha-Lipoic Acid Attenuates Cerebral Ischemia and Reperfusion Injury via Insulin Receptor and PI3K/Akt-Dependent Inhibition of NADPH Oxidase |
| title_full | Alpha-Lipoic Acid Attenuates Cerebral Ischemia and Reperfusion Injury via Insulin Receptor and PI3K/Akt-Dependent Inhibition of NADPH Oxidase |
| title_fullStr | Alpha-Lipoic Acid Attenuates Cerebral Ischemia and Reperfusion Injury via Insulin Receptor and PI3K/Akt-Dependent Inhibition of NADPH Oxidase |
| title_full_unstemmed | Alpha-Lipoic Acid Attenuates Cerebral Ischemia and Reperfusion Injury via Insulin Receptor and PI3K/Akt-Dependent Inhibition of NADPH Oxidase |
| title_short | Alpha-Lipoic Acid Attenuates Cerebral Ischemia and Reperfusion Injury via Insulin Receptor and PI3K/Akt-Dependent Inhibition of NADPH Oxidase |
| title_sort | alpha lipoic acid attenuates cerebral ischemia and reperfusion injury via insulin receptor and pi3k akt dependent inhibition of nadph oxidase |
| url | http://dx.doi.org/10.1155/2015/903186 |
| work_keys_str_mv | AT yinhuadong alphalipoicacidattenuatescerebralischemiaandreperfusioninjuryviainsulinreceptorandpi3kaktdependentinhibitionofnadphoxidase AT hongxinwang alphalipoicacidattenuatescerebralischemiaandreperfusioninjuryviainsulinreceptorandpi3kaktdependentinhibitionofnadphoxidase AT zefengchen alphalipoicacidattenuatescerebralischemiaandreperfusioninjuryviainsulinreceptorandpi3kaktdependentinhibitionofnadphoxidase |