Polymorphonuclear Leukocytes or Hydrogen Peroxide Enhance Biofilm Development of Mucoid Pseudomonas aeruginosa
Pseudomonas aeruginosa is an opportunistic pathogenic bacterium involved in many human infections, including pneumonia, diabetic foot ulcers, and ventilator-associated pneumonia. P. aeruginosa cells usually undergo mucoid conversion during chronic lung infection in patients with cystic fibrosis (CF)...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/8151362 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561774855979008 |
|---|---|
| author | Qi Tan Qing Ai Qi Xu Fang Li Jialin Yu |
| author_facet | Qi Tan Qing Ai Qi Xu Fang Li Jialin Yu |
| author_sort | Qi Tan |
| collection | DOAJ |
| description | Pseudomonas aeruginosa is an opportunistic pathogenic bacterium involved in many human infections, including pneumonia, diabetic foot ulcers, and ventilator-associated pneumonia. P. aeruginosa cells usually undergo mucoid conversion during chronic lung infection in patients with cystic fibrosis (CF) and resist destruction by polymorphonuclear leukocytes (PMNs), which release free oxygen radicals (ROS), such as H2O2. PMNs are the main leucocytes in the CF sputum of patients who are infected with P. aeruginosa, which usually forms biofilms. Here, we report that PMNs or H2O2 can promote biofilm formation by mucoid P. aeruginosa FRD1 with the use of the hanging-peg method. The mucoid strain infecting CF patients overproduces alginate. In this study, PMNs and H2O2 promoted alginate production, and biofilms treated with PMNs or H2O2 exhibited higher expression of alginate genes. Additionally, PMNs increased the activity of GDP-mannose dehydrogenase, which is the key enzyme in alginate biosynthesis. Our results demonstrate that PMNs or H2O2 can enhance mucoid P. aeruginosa biofilms. |
| format | Article |
| id | doaj-art-7acc4d81b565413fa1a26d6e3fd58c40 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-7acc4d81b565413fa1a26d6e3fd58c402025-02-03T01:24:14ZengWileyMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/81513628151362Polymorphonuclear Leukocytes or Hydrogen Peroxide Enhance Biofilm Development of Mucoid Pseudomonas aeruginosaQi Tan0Qing Ai1Qi Xu2Fang Li3Jialin Yu4Department of Neonatology, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Neonatology, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Neonatology, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Neonatology, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Neonatology, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaPseudomonas aeruginosa is an opportunistic pathogenic bacterium involved in many human infections, including pneumonia, diabetic foot ulcers, and ventilator-associated pneumonia. P. aeruginosa cells usually undergo mucoid conversion during chronic lung infection in patients with cystic fibrosis (CF) and resist destruction by polymorphonuclear leukocytes (PMNs), which release free oxygen radicals (ROS), such as H2O2. PMNs are the main leucocytes in the CF sputum of patients who are infected with P. aeruginosa, which usually forms biofilms. Here, we report that PMNs or H2O2 can promote biofilm formation by mucoid P. aeruginosa FRD1 with the use of the hanging-peg method. The mucoid strain infecting CF patients overproduces alginate. In this study, PMNs and H2O2 promoted alginate production, and biofilms treated with PMNs or H2O2 exhibited higher expression of alginate genes. Additionally, PMNs increased the activity of GDP-mannose dehydrogenase, which is the key enzyme in alginate biosynthesis. Our results demonstrate that PMNs or H2O2 can enhance mucoid P. aeruginosa biofilms.http://dx.doi.org/10.1155/2018/8151362 |
| spellingShingle | Qi Tan Qing Ai Qi Xu Fang Li Jialin Yu Polymorphonuclear Leukocytes or Hydrogen Peroxide Enhance Biofilm Development of Mucoid Pseudomonas aeruginosa Mediators of Inflammation |
| title | Polymorphonuclear Leukocytes or Hydrogen Peroxide Enhance Biofilm Development of Mucoid Pseudomonas aeruginosa |
| title_full | Polymorphonuclear Leukocytes or Hydrogen Peroxide Enhance Biofilm Development of Mucoid Pseudomonas aeruginosa |
| title_fullStr | Polymorphonuclear Leukocytes or Hydrogen Peroxide Enhance Biofilm Development of Mucoid Pseudomonas aeruginosa |
| title_full_unstemmed | Polymorphonuclear Leukocytes or Hydrogen Peroxide Enhance Biofilm Development of Mucoid Pseudomonas aeruginosa |
| title_short | Polymorphonuclear Leukocytes or Hydrogen Peroxide Enhance Biofilm Development of Mucoid Pseudomonas aeruginosa |
| title_sort | polymorphonuclear leukocytes or hydrogen peroxide enhance biofilm development of mucoid pseudomonas aeruginosa |
| url | http://dx.doi.org/10.1155/2018/8151362 |
| work_keys_str_mv | AT qitan polymorphonuclearleukocytesorhydrogenperoxideenhancebiofilmdevelopmentofmucoidpseudomonasaeruginosa AT qingai polymorphonuclearleukocytesorhydrogenperoxideenhancebiofilmdevelopmentofmucoidpseudomonasaeruginosa AT qixu polymorphonuclearleukocytesorhydrogenperoxideenhancebiofilmdevelopmentofmucoidpseudomonasaeruginosa AT fangli polymorphonuclearleukocytesorhydrogenperoxideenhancebiofilmdevelopmentofmucoidpseudomonasaeruginosa AT jialinyu polymorphonuclearleukocytesorhydrogenperoxideenhancebiofilmdevelopmentofmucoidpseudomonasaeruginosa |