Clonal Spread and Genetic Mechanisms Underpinning Ciprofloxacin Resistance in <i>Salmonella enteritidis</i>
<i>Salmonella enteritidis</i> is a major cause of foodborne illness worldwide, and the emergence of ciprofloxacin-resistant strains poses a significant threat to food safety and public health. This study aimed to investigate the prevalence, spread, and mechanisms of ciprofloxacin resista...
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2025-01-01
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| author | Zengfeng Zhang Hang Zhao Chunlei Shi |
| author_facet | Zengfeng Zhang Hang Zhao Chunlei Shi |
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| description | <i>Salmonella enteritidis</i> is a major cause of foodborne illness worldwide, and the emergence of ciprofloxacin-resistant strains poses a significant threat to food safety and public health. This study aimed to investigate the prevalence, spread, and mechanisms of ciprofloxacin resistance in <i>S. enteritidis</i> isolates from food and patient samples in Shanghai, China. A total of 1625 <i>S. enteritidis</i> isolates were screened, and 34 (2.1%) exhibited resistance to ciprofloxacin. Pulsed-field gel electrophoresis (PFGE) results suggested that clonal spread might have persisted among these 34 isolates in the local area for several years. Multiple plasmid-mediated quinolone resistance (PMQR) genes, GyrA mutations in the quinolone resistance-determining region (QRDR), and overexpression of RND efflux pumps were identified as potential contributors to ciprofloxacin resistance. PMQR genes <i>oqx</i>AB, <i>qnr</i>A, <i>qnr</i>B, and <i>aac</i>(6’)-Ib-cr as well as GyrA mutations S83Y, S83R, D87Y, D87G, D87N, and S83Y-D87Y were identified. The co-transfer of the PMQR gene <i>oqx</i>AB with the ESBL gene <i>bla</i><sub>CTX-M-14/55</sub> on an IncHI2 plasmid with a size of ~245 kbp was observed through conjugation, highlighting the role of horizontal gene transfer in the dissemination of antibiotic resistance. Sequencing of the <i>oqx</i>AB-bearing plasmid p12519A revealed a 248,746 bp sequence with a typical IncHI2 backbone. A 53,104 bp multidrug resistance region (MRR) was identified, containing two key antibiotic resistance determinants: IS<i>26</i>-<i>oqx</i>R-<i>oqx</i>AB-IS<i>26</i> and IS<i>26</i>-ΔIS<i>Ecp1</i>-<i>bla</i><sub>CTX-M-14</sub>-IS<i>903B</i>. The findings of this study indicate that ciprofloxacin-resistant <i>S. Enteritidis</i> poses a significant threat to food safety and public health. The persistence of clonal spread and the horizontal transfer of resistance genes highlight the need for enhanced surveillance and control measures to prevent the further spread of antibiotic resistance. |
| format | Article |
| id | doaj-art-7abaf59a8fd140af9cde555dd20e9608 |
| institution | Kabale University |
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| spelling | doaj-art-7abaf59a8fd140af9cde555dd20e96082025-01-24T13:33:09ZengMDPI AGFoods2304-81582025-01-0114228910.3390/foods14020289Clonal Spread and Genetic Mechanisms Underpinning Ciprofloxacin Resistance in <i>Salmonella enteritidis</i>Zengfeng Zhang0Hang Zhao1Chunlei Shi2MOST-USDA Joint Research Center for Food Safety, School of Agriculture and Biology and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, ChinaMOST-USDA Joint Research Center for Food Safety, School of Agriculture and Biology and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, ChinaMOST-USDA Joint Research Center for Food Safety, School of Agriculture and Biology and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China<i>Salmonella enteritidis</i> is a major cause of foodborne illness worldwide, and the emergence of ciprofloxacin-resistant strains poses a significant threat to food safety and public health. This study aimed to investigate the prevalence, spread, and mechanisms of ciprofloxacin resistance in <i>S. enteritidis</i> isolates from food and patient samples in Shanghai, China. A total of 1625 <i>S. enteritidis</i> isolates were screened, and 34 (2.1%) exhibited resistance to ciprofloxacin. Pulsed-field gel electrophoresis (PFGE) results suggested that clonal spread might have persisted among these 34 isolates in the local area for several years. Multiple plasmid-mediated quinolone resistance (PMQR) genes, GyrA mutations in the quinolone resistance-determining region (QRDR), and overexpression of RND efflux pumps were identified as potential contributors to ciprofloxacin resistance. PMQR genes <i>oqx</i>AB, <i>qnr</i>A, <i>qnr</i>B, and <i>aac</i>(6’)-Ib-cr as well as GyrA mutations S83Y, S83R, D87Y, D87G, D87N, and S83Y-D87Y were identified. The co-transfer of the PMQR gene <i>oqx</i>AB with the ESBL gene <i>bla</i><sub>CTX-M-14/55</sub> on an IncHI2 plasmid with a size of ~245 kbp was observed through conjugation, highlighting the role of horizontal gene transfer in the dissemination of antibiotic resistance. Sequencing of the <i>oqx</i>AB-bearing plasmid p12519A revealed a 248,746 bp sequence with a typical IncHI2 backbone. A 53,104 bp multidrug resistance region (MRR) was identified, containing two key antibiotic resistance determinants: IS<i>26</i>-<i>oqx</i>R-<i>oqx</i>AB-IS<i>26</i> and IS<i>26</i>-ΔIS<i>Ecp1</i>-<i>bla</i><sub>CTX-M-14</sub>-IS<i>903B</i>. The findings of this study indicate that ciprofloxacin-resistant <i>S. Enteritidis</i> poses a significant threat to food safety and public health. The persistence of clonal spread and the horizontal transfer of resistance genes highlight the need for enhanced surveillance and control measures to prevent the further spread of antibiotic resistance.https://www.mdpi.com/2304-8158/14/2/289<i>Salmonella enteritidis</i>ciprofloxacin resistancePMQR<i>gyr</i>Aefflux pump |
| spellingShingle | Zengfeng Zhang Hang Zhao Chunlei Shi Clonal Spread and Genetic Mechanisms Underpinning Ciprofloxacin Resistance in <i>Salmonella enteritidis</i> Foods <i>Salmonella enteritidis</i> ciprofloxacin resistance PMQR <i>gyr</i>A efflux pump |
| title | Clonal Spread and Genetic Mechanisms Underpinning Ciprofloxacin Resistance in <i>Salmonella enteritidis</i> |
| title_full | Clonal Spread and Genetic Mechanisms Underpinning Ciprofloxacin Resistance in <i>Salmonella enteritidis</i> |
| title_fullStr | Clonal Spread and Genetic Mechanisms Underpinning Ciprofloxacin Resistance in <i>Salmonella enteritidis</i> |
| title_full_unstemmed | Clonal Spread and Genetic Mechanisms Underpinning Ciprofloxacin Resistance in <i>Salmonella enteritidis</i> |
| title_short | Clonal Spread and Genetic Mechanisms Underpinning Ciprofloxacin Resistance in <i>Salmonella enteritidis</i> |
| title_sort | clonal spread and genetic mechanisms underpinning ciprofloxacin resistance in i salmonella enteritidis i |
| topic | <i>Salmonella enteritidis</i> ciprofloxacin resistance PMQR <i>gyr</i>A efflux pump |
| url | https://www.mdpi.com/2304-8158/14/2/289 |
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