Apoptosis Transcriptional Profile Induced by Porphyromonas gingivalis HmuY
This study aimed at evaluating the transcriptional profile of apoptosis-related genes after in vitro stimulation of peripheral blood mononuclear cells (PBMCs) derived from individuals with periodontitis (P) and healthy nonperiodontitis (NP) control subjects with P. gingivalis HmuY protein. PBMCs fro...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2019/6758159 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832560042821287936 |
|---|---|
| author | Paulo C. Carvalho-Filho Lilia F. Moura-Costa Ana C. M. Pimentel Mabel P. P. Lopes Sibelle A. Freitas Patrícia M. Miranda Ryan S. Costa Camila A. V. Figueirêdo Roberto Meyer Isaac S. Gomes-Filho Teresa Olczak Márcia T. Xavier Soraya C. Trindade |
| author_facet | Paulo C. Carvalho-Filho Lilia F. Moura-Costa Ana C. M. Pimentel Mabel P. P. Lopes Sibelle A. Freitas Patrícia M. Miranda Ryan S. Costa Camila A. V. Figueirêdo Roberto Meyer Isaac S. Gomes-Filho Teresa Olczak Márcia T. Xavier Soraya C. Trindade |
| author_sort | Paulo C. Carvalho-Filho |
| collection | DOAJ |
| description | This study aimed at evaluating the transcriptional profile of apoptosis-related genes after in vitro stimulation of peripheral blood mononuclear cells (PBMCs) derived from individuals with periodontitis (P) and healthy nonperiodontitis (NP) control subjects with P. gingivalis HmuY protein. PBMCs from the P and NP groups were stimulated with HmuY P. gingivalis protein, and the expression of genes related to apoptosis was assessed by custom real-time polymerase chain reaction array (Custom RT2 PCR Array). Compared with the NP group, the P group showed low relative levels of apoptosis-related gene expression, downregulated for FAS, FAS ligand, TNFSF10 (TRAIL), BAK1, CASP9, and APAF1 after P. gingivalis HmuY protein stimulation. Furthermore, the P group exhibited low levels of relative gene expression, downregulated for CASP7 when the cells were not stimulated. Our data suggest that P. gingivalis HmuY protein might participate differently in the modulation of the intrinsic and extrinsic apoptosis pathways. |
| format | Article |
| id | doaj-art-7a810b0c49ff41edabcd22f58beb5edc |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-7a810b0c49ff41edabcd22f58beb5edc2025-02-03T01:28:35ZengWileyMediators of Inflammation0962-93511466-18612019-01-01201910.1155/2019/67581596758159Apoptosis Transcriptional Profile Induced by Porphyromonas gingivalis HmuYPaulo C. Carvalho-Filho0Lilia F. Moura-Costa1Ana C. M. Pimentel2Mabel P. P. Lopes3Sibelle A. Freitas4Patrícia M. Miranda5Ryan S. Costa6Camila A. V. Figueirêdo7Roberto Meyer8Isaac S. Gomes-Filho9Teresa Olczak10Márcia T. Xavier11Soraya C. Trindade12Department of Immunology, Federal University of Bahia, Bahia, BrazilDepartment of Immunology, Federal University of Bahia, Bahia, BrazilDepartment of Immunology, Federal University of Bahia, Bahia, BrazilDepartment of Immunology, Federal University of Bahia, Bahia, BrazilDental School, Bahiana School of Medicine and Public Health, BrazilDepartment of Immunology, Federal University of Bahia, Bahia, BrazilDepartment of Immunology, Federal University of Bahia, Bahia, BrazilDepartment of Immunology, Federal University of Bahia, Bahia, BrazilDepartment of Immunology, Federal University of Bahia, Bahia, BrazilDepartment of Periodontics, Feira de Santana State University, Bahia, BrazilLaboratory of Medical Biology, Faculty of Biotechnology, University of Wroclaw, Wroclaw, PolandDental School, Bahiana School of Medicine and Public Health, BrazilDepartment of Immunology, Federal University of Bahia, Bahia, BrazilThis study aimed at evaluating the transcriptional profile of apoptosis-related genes after in vitro stimulation of peripheral blood mononuclear cells (PBMCs) derived from individuals with periodontitis (P) and healthy nonperiodontitis (NP) control subjects with P. gingivalis HmuY protein. PBMCs from the P and NP groups were stimulated with HmuY P. gingivalis protein, and the expression of genes related to apoptosis was assessed by custom real-time polymerase chain reaction array (Custom RT2 PCR Array). Compared with the NP group, the P group showed low relative levels of apoptosis-related gene expression, downregulated for FAS, FAS ligand, TNFSF10 (TRAIL), BAK1, CASP9, and APAF1 after P. gingivalis HmuY protein stimulation. Furthermore, the P group exhibited low levels of relative gene expression, downregulated for CASP7 when the cells were not stimulated. Our data suggest that P. gingivalis HmuY protein might participate differently in the modulation of the intrinsic and extrinsic apoptosis pathways.http://dx.doi.org/10.1155/2019/6758159 |
| spellingShingle | Paulo C. Carvalho-Filho Lilia F. Moura-Costa Ana C. M. Pimentel Mabel P. P. Lopes Sibelle A. Freitas Patrícia M. Miranda Ryan S. Costa Camila A. V. Figueirêdo Roberto Meyer Isaac S. Gomes-Filho Teresa Olczak Márcia T. Xavier Soraya C. Trindade Apoptosis Transcriptional Profile Induced by Porphyromonas gingivalis HmuY Mediators of Inflammation |
| title | Apoptosis Transcriptional Profile Induced by Porphyromonas gingivalis HmuY |
| title_full | Apoptosis Transcriptional Profile Induced by Porphyromonas gingivalis HmuY |
| title_fullStr | Apoptosis Transcriptional Profile Induced by Porphyromonas gingivalis HmuY |
| title_full_unstemmed | Apoptosis Transcriptional Profile Induced by Porphyromonas gingivalis HmuY |
| title_short | Apoptosis Transcriptional Profile Induced by Porphyromonas gingivalis HmuY |
| title_sort | apoptosis transcriptional profile induced by porphyromonas gingivalis hmuy |
| url | http://dx.doi.org/10.1155/2019/6758159 |
| work_keys_str_mv | AT pauloccarvalhofilho apoptosistranscriptionalprofileinducedbyporphyromonasgingivalishmuy AT liliafmouracosta apoptosistranscriptionalprofileinducedbyporphyromonasgingivalishmuy AT anacmpimentel apoptosistranscriptionalprofileinducedbyporphyromonasgingivalishmuy AT mabelpplopes apoptosistranscriptionalprofileinducedbyporphyromonasgingivalishmuy AT sibelleafreitas apoptosistranscriptionalprofileinducedbyporphyromonasgingivalishmuy AT patriciammiranda apoptosistranscriptionalprofileinducedbyporphyromonasgingivalishmuy AT ryanscosta apoptosistranscriptionalprofileinducedbyporphyromonasgingivalishmuy AT camilaavfigueiredo apoptosistranscriptionalprofileinducedbyporphyromonasgingivalishmuy AT robertomeyer apoptosistranscriptionalprofileinducedbyporphyromonasgingivalishmuy AT isaacsgomesfilho apoptosistranscriptionalprofileinducedbyporphyromonasgingivalishmuy AT teresaolczak apoptosistranscriptionalprofileinducedbyporphyromonasgingivalishmuy AT marciatxavier apoptosistranscriptionalprofileinducedbyporphyromonasgingivalishmuy AT sorayactrindade apoptosistranscriptionalprofileinducedbyporphyromonasgingivalishmuy |