Performance improvements and increased ruminal microbial interactions in Angus heifers via supplementation with native rumen bacteria during high-grain challenge
Abstract Feedlot cattle may be subjected to digestive disorders, including ruminal acidosis, due to high concentration of grain in their diet. Therefore, novel feeding strategies are required to maximize animal performance and mitigate economic losses in the operation. This study employed a two-peri...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-86331-1 |
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| author | Fan Yang Madison T. Henniger Andrew S. Izzo Emily A. Melchior Brooke A. Clemmons Mary A. Oliver James R. Gaffney Cameron Martino Taylor B. Ault-Seay Miranda L. Striluk Jordan J. Embree Juan F. Cordero-Llarena Pierre-Yves Mulon David E. Anderson Mallory M. Embree Phillip R. Myer |
| author_facet | Fan Yang Madison T. Henniger Andrew S. Izzo Emily A. Melchior Brooke A. Clemmons Mary A. Oliver James R. Gaffney Cameron Martino Taylor B. Ault-Seay Miranda L. Striluk Jordan J. Embree Juan F. Cordero-Llarena Pierre-Yves Mulon David E. Anderson Mallory M. Embree Phillip R. Myer |
| author_sort | Fan Yang |
| collection | DOAJ |
| description | Abstract Feedlot cattle may be subjected to digestive disorders, including ruminal acidosis, due to high concentration of grain in their diet. Therefore, novel feeding strategies are required to maximize animal performance and mitigate economic losses in the operation. This study employed a two-period crossover design to assess the effect of direct ruminal administration of native rumen microorganisms (NRM) inoculation on cattle that underwent a high-grain challenge. The NRM inoculation consisted of six microorganisms (1.70 M CFU /day/animal) isolated from the rumen of healthy feedlot cattle: Succinivibrio dextrinosolvens ASCUSBF53, Prevotella albensis ASCUSBF41, Chordicoccus furentiruminis ASCUSBF65, Bacteroides xylanisolvens ASCUSBF52, Clostridium beijerinckii ASCUSBF26, and Syntrophococcus sp. ASCUSBF60. The trial consisted of 16 Angus heifers receiving NRM (n = 8) or a CON (CON = Carrier Buffer; n = 8) inoculation daily for 14-days as pre-challenge while on a high-grain diet and continued daily for a 21-day treatment period. The combined 35 days of microbial supplementation resulted in an improved average daily gain (ADG) of 29% (P = 0.037) and a tendency toward a 19% decrease in the feed efficiency metric, gain to feed ratio (G: F) (P = 0.055). Additionally, administration of NRM to animals on a high-grain diet, improved ruminal microbiome stability (P < 0.001), potentially encouraging the conversion of rumen lactate to propionate over time via the succinate pathway and alleviating metabolic stress. |
| format | Article |
| id | doaj-art-7a8094d4787f48d4b0d822c23e7a1aca |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-7a8094d4787f48d4b0d822c23e7a1aca2025-01-26T12:33:37ZengNature PortfolioScientific Reports2045-23222025-01-0115111510.1038/s41598-025-86331-1Performance improvements and increased ruminal microbial interactions in Angus heifers via supplementation with native rumen bacteria during high-grain challengeFan Yang0Madison T. Henniger1Andrew S. Izzo2Emily A. Melchior3Brooke A. Clemmons4Mary A. Oliver5James R. Gaffney6Cameron Martino7Taylor B. Ault-Seay8Miranda L. Striluk9Jordan J. Embree10Juan F. Cordero-Llarena11Pierre-Yves Mulon12David E. Anderson13Mallory M. Embree14Phillip R. Myer15Native Microbials, Inc.Department of Animal Science, University of TennesseeNative Microbials, Inc.Department of Animal Science, University of TennesseeDepartment of Animal Science, University of TennesseeDepartment of Animal Science, University of TennesseeNative Microbials, Inc.Native Microbials, Inc.Department of Animal Science, University of TennesseeNative Microbials, Inc.Native Microbials, Inc.Department of Animal Science, University of TennesseeCollege of Veterinary Medicine, University of TennesseeCollege of Veterinary Medicine, University of TennesseeNative Microbials, Inc.Department of Animal Science, University of TennesseeAbstract Feedlot cattle may be subjected to digestive disorders, including ruminal acidosis, due to high concentration of grain in their diet. Therefore, novel feeding strategies are required to maximize animal performance and mitigate economic losses in the operation. This study employed a two-period crossover design to assess the effect of direct ruminal administration of native rumen microorganisms (NRM) inoculation on cattle that underwent a high-grain challenge. The NRM inoculation consisted of six microorganisms (1.70 M CFU /day/animal) isolated from the rumen of healthy feedlot cattle: Succinivibrio dextrinosolvens ASCUSBF53, Prevotella albensis ASCUSBF41, Chordicoccus furentiruminis ASCUSBF65, Bacteroides xylanisolvens ASCUSBF52, Clostridium beijerinckii ASCUSBF26, and Syntrophococcus sp. ASCUSBF60. The trial consisted of 16 Angus heifers receiving NRM (n = 8) or a CON (CON = Carrier Buffer; n = 8) inoculation daily for 14-days as pre-challenge while on a high-grain diet and continued daily for a 21-day treatment period. The combined 35 days of microbial supplementation resulted in an improved average daily gain (ADG) of 29% (P = 0.037) and a tendency toward a 19% decrease in the feed efficiency metric, gain to feed ratio (G: F) (P = 0.055). Additionally, administration of NRM to animals on a high-grain diet, improved ruminal microbiome stability (P < 0.001), potentially encouraging the conversion of rumen lactate to propionate over time via the succinate pathway and alleviating metabolic stress.https://doi.org/10.1038/s41598-025-86331-1Ruminal acidosisGrain challengeBeef cattleNative rumen microorganism |
| spellingShingle | Fan Yang Madison T. Henniger Andrew S. Izzo Emily A. Melchior Brooke A. Clemmons Mary A. Oliver James R. Gaffney Cameron Martino Taylor B. Ault-Seay Miranda L. Striluk Jordan J. Embree Juan F. Cordero-Llarena Pierre-Yves Mulon David E. Anderson Mallory M. Embree Phillip R. Myer Performance improvements and increased ruminal microbial interactions in Angus heifers via supplementation with native rumen bacteria during high-grain challenge Scientific Reports Ruminal acidosis Grain challenge Beef cattle Native rumen microorganism |
| title | Performance improvements and increased ruminal microbial interactions in Angus heifers via supplementation with native rumen bacteria during high-grain challenge |
| title_full | Performance improvements and increased ruminal microbial interactions in Angus heifers via supplementation with native rumen bacteria during high-grain challenge |
| title_fullStr | Performance improvements and increased ruminal microbial interactions in Angus heifers via supplementation with native rumen bacteria during high-grain challenge |
| title_full_unstemmed | Performance improvements and increased ruminal microbial interactions in Angus heifers via supplementation with native rumen bacteria during high-grain challenge |
| title_short | Performance improvements and increased ruminal microbial interactions in Angus heifers via supplementation with native rumen bacteria during high-grain challenge |
| title_sort | performance improvements and increased ruminal microbial interactions in angus heifers via supplementation with native rumen bacteria during high grain challenge |
| topic | Ruminal acidosis Grain challenge Beef cattle Native rumen microorganism |
| url | https://doi.org/10.1038/s41598-025-86331-1 |
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