Sero-molecular prevalence of toxoplasmosis in hemodialysis and peritoneal dialysis patients in Markazi Province, Iran
Abstract Background Infectious diseases, particularly parasitic infections such as toxoplasmosis, contribute significantly to the morbidity and mortality of hemodialysis patients. Toxoplasma gondii infection poses serious risks, especially to immunocompromised individuals. This study aimed to assess...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | Tropical Medicine and Health |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41182-024-00676-3 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832594416690266112 |
|---|---|
| author | Hossein Sarmadian Mana Shojapour Fereshteh chegeni Mohammad Amin Tabatabaiefar Farshid Haghverdi Roham Sarmadian Reza Ghasemikhah |
| author_facet | Hossein Sarmadian Mana Shojapour Fereshteh chegeni Mohammad Amin Tabatabaiefar Farshid Haghverdi Roham Sarmadian Reza Ghasemikhah |
| author_sort | Hossein Sarmadian |
| collection | DOAJ |
| description | Abstract Background Infectious diseases, particularly parasitic infections such as toxoplasmosis, contribute significantly to the morbidity and mortality of hemodialysis patients. Toxoplasma gondii infection poses serious risks, especially to immunocompromised individuals. This study aimed to assess the prevalence of latent toxoplasmosis in dialysis patients in Markazi Province, Iran. Methods A total of 181 patients (168 hemodialysis and 13 peritoneal dialysis) were included in this cross-sectional study. Blood samples were collected and tested for anti-Toxoplasma IgG and IgM antibodies using ELISA, while PCR was used to detect T. gondii DNA. Data were analyzed using SPSS, with P < 0.05 considered significant. Results Out of 181 patients, 123 (67.95%) were seropositive for IgG antibodies, with the highest prevalence in those aged over 60 years. No cases tested positive for IgM antibodies or T. gondii DNA. Age was significantly associated with higher seroprevalence (P < 0.05), but there were no significant differences regarding gender, dialysis type, or duration of dialysis. Conclusion The high prevalence of latent toxoplasmosis in dialysis patients underscores the need for regular screening to prevent serious complications. Given the limitations of serological tests, molecular diagnostics like PCR should be considered for better detection in immunocompromised patients. |
| format | Article |
| id | doaj-art-7a60aeccfedc4076a5e08b13cae2ff95 |
| institution | Kabale University |
| issn | 1349-4147 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Tropical Medicine and Health |
| spelling | doaj-art-7a60aeccfedc4076a5e08b13cae2ff952025-01-19T12:39:09ZengBMCTropical Medicine and Health1349-41472025-01-015311510.1186/s41182-024-00676-3Sero-molecular prevalence of toxoplasmosis in hemodialysis and peritoneal dialysis patients in Markazi Province, IranHossein Sarmadian0Mana Shojapour1Fereshteh chegeni2Mohammad Amin Tabatabaiefar3Farshid Haghverdi4Roham Sarmadian5Reza Ghasemikhah6Department of Infectious Disease, School of Medicine, Arak University of Medical SciencesMolecular and Medicine Research Center, Arak University of Medical SciencesStudents Research Committee, Arak University of Medical SciencesDepartment of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical SciencesNephrology Department of Internal Medicine, School of Medicine, Amiralmomenin Hospital Arak University of Medical SciencesInfectious Diseases Research Center, Arak University of Medical SciencesInfectious Diseases Research Center, Arak University of Medical SciencesAbstract Background Infectious diseases, particularly parasitic infections such as toxoplasmosis, contribute significantly to the morbidity and mortality of hemodialysis patients. Toxoplasma gondii infection poses serious risks, especially to immunocompromised individuals. This study aimed to assess the prevalence of latent toxoplasmosis in dialysis patients in Markazi Province, Iran. Methods A total of 181 patients (168 hemodialysis and 13 peritoneal dialysis) were included in this cross-sectional study. Blood samples were collected and tested for anti-Toxoplasma IgG and IgM antibodies using ELISA, while PCR was used to detect T. gondii DNA. Data were analyzed using SPSS, with P < 0.05 considered significant. Results Out of 181 patients, 123 (67.95%) were seropositive for IgG antibodies, with the highest prevalence in those aged over 60 years. No cases tested positive for IgM antibodies or T. gondii DNA. Age was significantly associated with higher seroprevalence (P < 0.05), but there were no significant differences regarding gender, dialysis type, or duration of dialysis. Conclusion The high prevalence of latent toxoplasmosis in dialysis patients underscores the need for regular screening to prevent serious complications. Given the limitations of serological tests, molecular diagnostics like PCR should be considered for better detection in immunocompromised patients.https://doi.org/10.1186/s41182-024-00676-3ToxoplasmosisHemodialysisELISAPCR |
| spellingShingle | Hossein Sarmadian Mana Shojapour Fereshteh chegeni Mohammad Amin Tabatabaiefar Farshid Haghverdi Roham Sarmadian Reza Ghasemikhah Sero-molecular prevalence of toxoplasmosis in hemodialysis and peritoneal dialysis patients in Markazi Province, Iran Tropical Medicine and Health Toxoplasmosis Hemodialysis ELISA PCR |
| title | Sero-molecular prevalence of toxoplasmosis in hemodialysis and peritoneal dialysis patients in Markazi Province, Iran |
| title_full | Sero-molecular prevalence of toxoplasmosis in hemodialysis and peritoneal dialysis patients in Markazi Province, Iran |
| title_fullStr | Sero-molecular prevalence of toxoplasmosis in hemodialysis and peritoneal dialysis patients in Markazi Province, Iran |
| title_full_unstemmed | Sero-molecular prevalence of toxoplasmosis in hemodialysis and peritoneal dialysis patients in Markazi Province, Iran |
| title_short | Sero-molecular prevalence of toxoplasmosis in hemodialysis and peritoneal dialysis patients in Markazi Province, Iran |
| title_sort | sero molecular prevalence of toxoplasmosis in hemodialysis and peritoneal dialysis patients in markazi province iran |
| topic | Toxoplasmosis Hemodialysis ELISA PCR |
| url | https://doi.org/10.1186/s41182-024-00676-3 |
| work_keys_str_mv | AT hosseinsarmadian seromolecularprevalenceoftoxoplasmosisinhemodialysisandperitonealdialysispatientsinmarkaziprovinceiran AT manashojapour seromolecularprevalenceoftoxoplasmosisinhemodialysisandperitonealdialysispatientsinmarkaziprovinceiran AT fereshtehchegeni seromolecularprevalenceoftoxoplasmosisinhemodialysisandperitonealdialysispatientsinmarkaziprovinceiran AT mohammadamintabatabaiefar seromolecularprevalenceoftoxoplasmosisinhemodialysisandperitonealdialysispatientsinmarkaziprovinceiran AT farshidhaghverdi seromolecularprevalenceoftoxoplasmosisinhemodialysisandperitonealdialysispatientsinmarkaziprovinceiran AT rohamsarmadian seromolecularprevalenceoftoxoplasmosisinhemodialysisandperitonealdialysispatientsinmarkaziprovinceiran AT rezaghasemikhah seromolecularprevalenceoftoxoplasmosisinhemodialysisandperitonealdialysispatientsinmarkaziprovinceiran |