Differential diversity and structure of autotrophs in agricultural soils of Qinghai Province
ABSTRACT The biodiversity of CO2-assimilating bacterial communities is pivotal for carbon sequestration in agricultural systems. Changes in the diversity, structure, and activity of the soil chemolithoautotrophic bacteria were examined in four agricultural areas, Dulan (DL), Gonghe (GH), Huzhu (HZ),...
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American Society for Microbiology
2025-02-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02693-24 |
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| author | Lianyu Zhou Xuelan Ma Qiaoyu Luo Feng Qiao Huichun Xie Longrui Wang Wenjuan Sun Yu Liu Yun Ma |
| author_facet | Lianyu Zhou Xuelan Ma Qiaoyu Luo Feng Qiao Huichun Xie Longrui Wang Wenjuan Sun Yu Liu Yun Ma |
| author_sort | Lianyu Zhou |
| collection | DOAJ |
| description | ABSTRACT The biodiversity of CO2-assimilating bacterial communities is pivotal for carbon sequestration in agricultural systems. Changes in the diversity, structure, and activity of the soil chemolithoautotrophic bacteria were examined in four agricultural areas, Dulan (DL), Gonghe (GH), Huzhu (HZ), and Datong (DT) counties in Qinghai Province, where wheat, oilseed rape, and barley were planted. This process was performed using Illumina amplicon sequencing of the ribulose-1,5-bisphosphatecarboxylase/oxygenase (RubisCO) gene (cbbL Form I) and activity data. The diversity, community, and activity of soil autotrophic CO2-fixing bacteria differed significantly across soil sites, whereas cbbL-bearing bacterial diversity and activity were similar across different crop types. RubisCO activity in the HZ region was significantly greater than in the other three regions (P < 0.001). The overall relative abundance trend of the bacterial taxa was similar among the three crop samples. Moreover, 31, 27, 10, and 8 significant linear discriminant analysis effect sizes were identified in the four regions collected from HZ, DL, DT, and GH, respectively. No significant biomarkers were detected in any of the crop groups. Some soil properties had significant relationships with the autotrophic bacterial community composition.IMPORTANCEAgricultural soil plays important roles in carbon fixation during carbon capture and storage. Autotrophic bacteria that utilize inorganic compounds as electron donors for growth fix CO2 photosynthetically or chemo-autotrophically in diverse ecosystems and affect soil organic carbon sequestration. Soil properties, agronomic management measures, and environmental factors can influence the community composition, abundance, and activity of CO2-assimilating bacteria. This study aims at evaluating the effects of different regions and crop types on the abundance, composition, and activity of CO2-fixing bacteria in agricultural soil. |
| format | Article |
| id | doaj-art-7a53c32a146a4101a906e7bfc45784e4 |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj-art-7a53c32a146a4101a906e7bfc45784e42025-02-04T14:03:40ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-02-0113210.1128/spectrum.02693-24Differential diversity and structure of autotrophs in agricultural soils of Qinghai ProvinceLianyu Zhou0Xuelan Ma1Qiaoyu Luo2Feng Qiao3Huichun Xie4Longrui Wang5Wenjuan Sun6Yu Liu7Yun Ma8Key Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau in Qinghai Province, Academy of Plateau Science and Sustainability, School of Life Science, Qinghai Normal University, Xining, ChinaKey Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau in Qinghai Province, Academy of Plateau Science and Sustainability, School of Life Science, Qinghai Normal University, Xining, ChinaKey Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau in Qinghai Province, Academy of Plateau Science and Sustainability, School of Life Science, Qinghai Normal University, Xining, ChinaKey Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau in Qinghai Province, Academy of Plateau Science and Sustainability, School of Life Science, Qinghai Normal University, Xining, ChinaKey Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau in Qinghai Province, Academy of Plateau Science and Sustainability, School of Life Science, Qinghai Normal University, Xining, ChinaKey Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau in Qinghai Province, Academy of Plateau Science and Sustainability, School of Life Science, Qinghai Normal University, Xining, ChinaKey Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau in Qinghai Province, Academy of Plateau Science and Sustainability, School of Life Science, Qinghai Normal University, Xining, ChinaKey Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau in Qinghai Province, Academy of Plateau Science and Sustainability, School of Life Science, Qinghai Normal University, Xining, ChinaKey Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau in Qinghai Province, Academy of Plateau Science and Sustainability, School of Life Science, Qinghai Normal University, Xining, ChinaABSTRACT The biodiversity of CO2-assimilating bacterial communities is pivotal for carbon sequestration in agricultural systems. Changes in the diversity, structure, and activity of the soil chemolithoautotrophic bacteria were examined in four agricultural areas, Dulan (DL), Gonghe (GH), Huzhu (HZ), and Datong (DT) counties in Qinghai Province, where wheat, oilseed rape, and barley were planted. This process was performed using Illumina amplicon sequencing of the ribulose-1,5-bisphosphatecarboxylase/oxygenase (RubisCO) gene (cbbL Form I) and activity data. The diversity, community, and activity of soil autotrophic CO2-fixing bacteria differed significantly across soil sites, whereas cbbL-bearing bacterial diversity and activity were similar across different crop types. RubisCO activity in the HZ region was significantly greater than in the other three regions (P < 0.001). The overall relative abundance trend of the bacterial taxa was similar among the three crop samples. Moreover, 31, 27, 10, and 8 significant linear discriminant analysis effect sizes were identified in the four regions collected from HZ, DL, DT, and GH, respectively. No significant biomarkers were detected in any of the crop groups. Some soil properties had significant relationships with the autotrophic bacterial community composition.IMPORTANCEAgricultural soil plays important roles in carbon fixation during carbon capture and storage. Autotrophic bacteria that utilize inorganic compounds as electron donors for growth fix CO2 photosynthetically or chemo-autotrophically in diverse ecosystems and affect soil organic carbon sequestration. Soil properties, agronomic management measures, and environmental factors can influence the community composition, abundance, and activity of CO2-assimilating bacteria. This study aims at evaluating the effects of different regions and crop types on the abundance, composition, and activity of CO2-fixing bacteria in agricultural soil.https://journals.asm.org/doi/10.1128/spectrum.02693-24agricultural soilautotrophic microorganismcarbon fixation potentialcommunity structureRubisCO activity |
| spellingShingle | Lianyu Zhou Xuelan Ma Qiaoyu Luo Feng Qiao Huichun Xie Longrui Wang Wenjuan Sun Yu Liu Yun Ma Differential diversity and structure of autotrophs in agricultural soils of Qinghai Province Microbiology Spectrum agricultural soil autotrophic microorganism carbon fixation potential community structure RubisCO activity |
| title | Differential diversity and structure of autotrophs in agricultural soils of Qinghai Province |
| title_full | Differential diversity and structure of autotrophs in agricultural soils of Qinghai Province |
| title_fullStr | Differential diversity and structure of autotrophs in agricultural soils of Qinghai Province |
| title_full_unstemmed | Differential diversity and structure of autotrophs in agricultural soils of Qinghai Province |
| title_short | Differential diversity and structure of autotrophs in agricultural soils of Qinghai Province |
| title_sort | differential diversity and structure of autotrophs in agricultural soils of qinghai province |
| topic | agricultural soil autotrophic microorganism carbon fixation potential community structure RubisCO activity |
| url | https://journals.asm.org/doi/10.1128/spectrum.02693-24 |
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