Determination of the Strength of Adhesion between Lipid Vesicles
A commonly used method to determine the strength of adhesion between adhering lipid vesicles is measuring their effective contact angle from experimental images. The aim of this paper is to estimate the interobserver variations in vesicles effective contact angle measurements and to propose a new me...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/146804 |
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| author | Tomáš Mareš Matej Daniel Aleš Iglič Veronika Kralj-Iglič Miha Fošnarič |
| author_facet | Tomáš Mareš Matej Daniel Aleš Iglič Veronika Kralj-Iglič Miha Fošnarič |
| author_sort | Tomáš Mareš |
| collection | DOAJ |
| description | A commonly used method to determine the strength of adhesion between adhering lipid vesicles is measuring their effective contact angle from experimental images. The aim of this paper is to estimate the interobserver variations in vesicles effective contact angle measurements and to propose a new method for estimating the strength of membrane vesicle adhesion. Theoretical model shows for the old and for the new measure a monotonic dependence on the strength of adhesion. Results obtained by both measuring techniques show statistically significant correlation and high interobserver reliability for both methods. Therefore the conventional method of measuring the effective contact angle gives qualitatively relevant results as the measure of the lipid vesicle adhesion. However, the new measuring technique provides a lower variation of the measured values than the conventional measures using the effective contact angle. Moreover, obtaining the adhesion angle can be automatized more easily than obtaining the effective contact angle. |
| format | Article |
| id | doaj-art-7a42a698954041ac8776e17eb4211fcf |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-7a42a698954041ac8776e17eb4211fcf2025-02-03T05:51:58ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/146804146804Determination of the Strength of Adhesion between Lipid VesiclesTomáš Mareš0Matej Daniel1Aleš Iglič2Veronika Kralj-Iglič3Miha Fošnarič4Laboratory of Biomechanics, Faculty of Mechanical Engineering, CTU in Prague, Technická 4, 166 07 Prague 6, Czech RepublicLaboratory of Biomechanics, Faculty of Mechanical Engineering, CTU in Prague, Technická 4, 166 07 Prague 6, Czech RepublicLaboratory of Biophysics, Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, 1000 Ljubljana, SloveniaLaboratory of Clinical Biophysics, Faculty of Medicine, University of Ljubljana, Lipičeva 2, 1000 Ljubljana, SloveniaLaboratory of Biophysics, Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, 1000 Ljubljana, SloveniaA commonly used method to determine the strength of adhesion between adhering lipid vesicles is measuring their effective contact angle from experimental images. The aim of this paper is to estimate the interobserver variations in vesicles effective contact angle measurements and to propose a new method for estimating the strength of membrane vesicle adhesion. Theoretical model shows for the old and for the new measure a monotonic dependence on the strength of adhesion. Results obtained by both measuring techniques show statistically significant correlation and high interobserver reliability for both methods. Therefore the conventional method of measuring the effective contact angle gives qualitatively relevant results as the measure of the lipid vesicle adhesion. However, the new measuring technique provides a lower variation of the measured values than the conventional measures using the effective contact angle. Moreover, obtaining the adhesion angle can be automatized more easily than obtaining the effective contact angle.http://dx.doi.org/10.1100/2012/146804 |
| spellingShingle | Tomáš Mareš Matej Daniel Aleš Iglič Veronika Kralj-Iglič Miha Fošnarič Determination of the Strength of Adhesion between Lipid Vesicles The Scientific World Journal |
| title | Determination of the Strength of Adhesion between Lipid Vesicles |
| title_full | Determination of the Strength of Adhesion between Lipid Vesicles |
| title_fullStr | Determination of the Strength of Adhesion between Lipid Vesicles |
| title_full_unstemmed | Determination of the Strength of Adhesion between Lipid Vesicles |
| title_short | Determination of the Strength of Adhesion between Lipid Vesicles |
| title_sort | determination of the strength of adhesion between lipid vesicles |
| url | http://dx.doi.org/10.1100/2012/146804 |
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