“Lung-on-a-chip” as an instrument for studying the pathophysiology of human respiration
“Lung-on-a-chip” (LoC) is a microfluidic device, imitating the gas-fluid interface of the pulmonary alveole in the human lung and intended for pathophysiological, pharmacological and molecular-biological studies of the air-blood barrier in vitro. The LoC device itself contains a system of fluid and...
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Eco-vector
2024-12-01
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| Series: | Клиническая практика |
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| Online Access: | https://journals.eco-vector.com/clinpractice/article/viewFile/637140/pdf |
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| author | Oksana A. Zhukova Iuliia V. Ozerskaya Dmitry V. Basmanov Vsevolod Yu. Stolyarov Vladimir G. Bogush Vladimir V. Kolesov Kirill A. Zykov Gaukhar M. Yusubalieva Vladimir P. Baklaushev |
| author_facet | Oksana A. Zhukova Iuliia V. Ozerskaya Dmitry V. Basmanov Vsevolod Yu. Stolyarov Vladimir G. Bogush Vladimir V. Kolesov Kirill A. Zykov Gaukhar M. Yusubalieva Vladimir P. Baklaushev |
| author_sort | Oksana A. Zhukova |
| collection | DOAJ |
| description | “Lung-on-a-chip” (LoC) is a microfluidic device, imitating the gas-fluid interface of the pulmonary alveole in the human lung and intended for pathophysiological, pharmacological and molecular-biological studies of the air-blood barrier in vitro. The LoC device itself contains a system of fluid and gas microchannels, separated with a semipermeable elastic membrane, containing a polymer base and the alveolar cell elements. Depending on the type of LoC (single-, double- and three-channel), the membrane may contain only alveolocytes or alveolocytes combined with other cells — endotheliocytes, fibroblasts, alveolar macrophages or tumor cells. Some LoC models also include proteinic or hydrogel stroma, imitating the pulmonary interstitium. The first double-channel LoC variant, in which one side of the membrane contained an alveolocytic monolayer and the other side — a monolayer of endotheliocytes, was developed in 2010 by a group of scientists from the Harvard University for maximally precise in vitro reproduction of the micro-environment and biomechanics operations of the alveoli. Modern LoC modifications include the same elements and differ only by the construction of the microfluidic system, by the biomaterial of semipermeable membrane, by the composition of cellular and stromal elements and by specific tasks to be solved. Besides the LoC imitating the hematoalveolar barrier, there are modifications for studying the specific pathophysiological processes, for the screening of medicinal products, for modeling specific diseases, for example, lung cancer, chronic obstructive pulmonary disease or asthma. In the present review, we have analyzed the existing types of LoC, the biomaterials used, the methods of detecting molecular processes within the microfluidic devices and the main directions of research to be conducted using the “lung-on-a-chip”. |
| format | Article |
| id | doaj-art-7a2844f9332a44b49939090b103581ba |
| institution | Kabale University |
| issn | 2220-3095 2618-8627 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Eco-vector |
| record_format | Article |
| series | Клиническая практика |
| spelling | doaj-art-7a2844f9332a44b49939090b103581ba2025-01-27T21:32:21ZengEco-vectorКлиническая практика2220-30952618-86272024-12-01154708810.17816/clinpract63714078452“Lung-on-a-chip” as an instrument for studying the pathophysiology of human respirationOksana A. Zhukova0https://orcid.org/0000-0002-0907-0078Iuliia V. Ozerskaya1https://orcid.org/0009-0008-4893-2735Dmitry V. Basmanov2https://orcid.org/0000-0001-6620-7360Vsevolod Yu. Stolyarov3https://orcid.org/0009-0002-9168-9378Vladimir G. Bogush4https://orcid.org/0000-0002-7159-0381Vladimir V. Kolesov5https://orcid.org/0000-0001-6427-6362Kirill A. Zykov6https://orcid.org/0000-0003-3385-2632Gaukhar M. Yusubalieva7https://orcid.org/0000-0003-3056-4889Vladimir P. Baklaushev8https://orcid.org/0000-0003-1039-4245Pulmonology Scientific Research InstitutePulmonology Scientific Research InstituteLopukhin Federal Research and Clinical Center of Physical-Chemical MedicineLopukhin Federal Research and Clinical Center of Physical-Chemical MedicineNational Research Center «Kurchatov Institute»Kotelnikov Institute of Radio Engineering and ElectronicsPulmonology Scientific Research InstituteFederal Center of Brain Research and NeurotechnologiesPulmonology Scientific Research Institute“Lung-on-a-chip” (LoC) is a microfluidic device, imitating the gas-fluid interface of the pulmonary alveole in the human lung and intended for pathophysiological, pharmacological and molecular-biological studies of the air-blood barrier in vitro. The LoC device itself contains a system of fluid and gas microchannels, separated with a semipermeable elastic membrane, containing a polymer base and the alveolar cell elements. Depending on the type of LoC (single-, double- and three-channel), the membrane may contain only alveolocytes or alveolocytes combined with other cells — endotheliocytes, fibroblasts, alveolar macrophages or tumor cells. Some LoC models also include proteinic or hydrogel stroma, imitating the pulmonary interstitium. The first double-channel LoC variant, in which one side of the membrane contained an alveolocytic monolayer and the other side — a monolayer of endotheliocytes, was developed in 2010 by a group of scientists from the Harvard University for maximally precise in vitro reproduction of the micro-environment and biomechanics operations of the alveoli. Modern LoC modifications include the same elements and differ only by the construction of the microfluidic system, by the biomaterial of semipermeable membrane, by the composition of cellular and stromal elements and by specific tasks to be solved. Besides the LoC imitating the hematoalveolar barrier, there are modifications for studying the specific pathophysiological processes, for the screening of medicinal products, for modeling specific diseases, for example, lung cancer, chronic obstructive pulmonary disease or asthma. In the present review, we have analyzed the existing types of LoC, the biomaterials used, the methods of detecting molecular processes within the microfluidic devices and the main directions of research to be conducted using the “lung-on-a-chip”.https://journals.eco-vector.com/clinpractice/article/viewFile/637140/pdflung-on-a-chipblood-alveolar barrierrespiratory diseasesmicrofluidic devices |
| spellingShingle | Oksana A. Zhukova Iuliia V. Ozerskaya Dmitry V. Basmanov Vsevolod Yu. Stolyarov Vladimir G. Bogush Vladimir V. Kolesov Kirill A. Zykov Gaukhar M. Yusubalieva Vladimir P. Baklaushev “Lung-on-a-chip” as an instrument for studying the pathophysiology of human respiration Клиническая практика lung-on-a-chip blood-alveolar barrier respiratory diseases microfluidic devices |
| title | “Lung-on-a-chip” as an instrument for studying the pathophysiology of human respiration |
| title_full | “Lung-on-a-chip” as an instrument for studying the pathophysiology of human respiration |
| title_fullStr | “Lung-on-a-chip” as an instrument for studying the pathophysiology of human respiration |
| title_full_unstemmed | “Lung-on-a-chip” as an instrument for studying the pathophysiology of human respiration |
| title_short | “Lung-on-a-chip” as an instrument for studying the pathophysiology of human respiration |
| title_sort | lung on a chip as an instrument for studying the pathophysiology of human respiration |
| topic | lung-on-a-chip blood-alveolar barrier respiratory diseases microfluidic devices |
| url | https://journals.eco-vector.com/clinpractice/article/viewFile/637140/pdf |
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