Resveratrol-loaded PCL-PEG/GO/HAP biocomposite bone membranes: Evaluation of mechanical properties, release kinetics, and cellular response
In this study, biocomposite membranes were developed by incorporating resveratrol (RSV)-loaded PCL-PEG composites, modified with graphene oxide (GO) and hydroxyapatite (HAP). The aim was to enhance hydrophilicity with GO and improve bioactivity with HAP. The release kinetics of RSV was evaluated by...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2025-02-01
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| Series: | Journal of Applied Biomaterials & Functional Materials |
| Online Access: | https://doi.org/10.1177/22808000251314087 |
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| author | Betül Meryem Arpacay Fatih Ciftci Ali Can Özarslan Mustafa Unal Mine Kucak Aslihan Yelkenci |
| author_facet | Betül Meryem Arpacay Fatih Ciftci Ali Can Özarslan Mustafa Unal Mine Kucak Aslihan Yelkenci |
| author_sort | Betül Meryem Arpacay |
| collection | DOAJ |
| description | In this study, biocomposite membranes were developed by incorporating resveratrol (RSV)-loaded PCL-PEG composites, modified with graphene oxide (GO) and hydroxyapatite (HAP). The aim was to enhance hydrophilicity with GO and improve bioactivity with HAP. The release kinetics of RSV was evaluated by using Franz diffusion cells and compared with various kinetic models, including Korsmeyer-Peppas, Higuchi, and Baker, all of which showed high correlation coefficients ( R ²) close to 0.99. Mechanical tests was performed to determine the suitability of these membranes for tissue engineering applications. The composite membrane modified with GO and HAP exhibited tensile strength of 105.2 ± 5.8 MPa, tensile modulus of 3895 ± 159 MPa, elongation at break of 8.4 ± 0.9%, and toughness of 5.88 ± 0.46 MJ/m³. In vitro cell adhesion studies, visualized using DAPI fluorescence staining, demonstrated increased cell adhesion to the composite membranes over periods of 1, 3, 5, 7, and 14 days. These findings highlight the potential of the RSV-loaded PCL-PEG membranes, enhanced with GO and HAP, for applications in bone tissue engineering. |
| format | Article |
| id | doaj-art-79e0483df01a40f5a5f7e9861430b8b2 |
| institution | Kabale University |
| issn | 2280-8000 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Applied Biomaterials & Functional Materials |
| spelling | doaj-art-79e0483df01a40f5a5f7e9861430b8b22025-02-03T06:03:20ZengSAGE PublishingJournal of Applied Biomaterials & Functional Materials2280-80002025-02-012310.1177/22808000251314087Resveratrol-loaded PCL-PEG/GO/HAP biocomposite bone membranes: Evaluation of mechanical properties, release kinetics, and cellular responseBetül Meryem Arpacay0Fatih Ciftci1Ali Can Özarslan2Mustafa Unal3Mine Kucak4Aslihan Yelkenci5Department of Biomedical Engineering, Fatih Sultan Mehmet Vakıf University, Istanbul, TurkeyDepartment of Technology Transfer Office, Fatih Sultan Mehmet Vakıf University, Istanbul, TurkeyHealth Biotechnology Joint Research and Application Center of Excellence, Istanbul, TurkeyCenter for Advanced Orthopedics Studies, Beth Israel Deaconess Medical Center, Boston, MA, USADepartment of Molecular Biology and Genetics, Yildiz Technical University, Istanbul, TurkeyFaculty of Dentistry, Department of Pediatric Dentistry, University of Health Sciences, Istanbul, TurkeyIn this study, biocomposite membranes were developed by incorporating resveratrol (RSV)-loaded PCL-PEG composites, modified with graphene oxide (GO) and hydroxyapatite (HAP). The aim was to enhance hydrophilicity with GO and improve bioactivity with HAP. The release kinetics of RSV was evaluated by using Franz diffusion cells and compared with various kinetic models, including Korsmeyer-Peppas, Higuchi, and Baker, all of which showed high correlation coefficients ( R ²) close to 0.99. Mechanical tests was performed to determine the suitability of these membranes for tissue engineering applications. The composite membrane modified with GO and HAP exhibited tensile strength of 105.2 ± 5.8 MPa, tensile modulus of 3895 ± 159 MPa, elongation at break of 8.4 ± 0.9%, and toughness of 5.88 ± 0.46 MJ/m³. In vitro cell adhesion studies, visualized using DAPI fluorescence staining, demonstrated increased cell adhesion to the composite membranes over periods of 1, 3, 5, 7, and 14 days. These findings highlight the potential of the RSV-loaded PCL-PEG membranes, enhanced with GO and HAP, for applications in bone tissue engineering.https://doi.org/10.1177/22808000251314087 |
| spellingShingle | Betül Meryem Arpacay Fatih Ciftci Ali Can Özarslan Mustafa Unal Mine Kucak Aslihan Yelkenci Resveratrol-loaded PCL-PEG/GO/HAP biocomposite bone membranes: Evaluation of mechanical properties, release kinetics, and cellular response Journal of Applied Biomaterials & Functional Materials |
| title | Resveratrol-loaded PCL-PEG/GO/HAP biocomposite bone membranes: Evaluation of mechanical properties, release kinetics, and cellular response |
| title_full | Resveratrol-loaded PCL-PEG/GO/HAP biocomposite bone membranes: Evaluation of mechanical properties, release kinetics, and cellular response |
| title_fullStr | Resveratrol-loaded PCL-PEG/GO/HAP biocomposite bone membranes: Evaluation of mechanical properties, release kinetics, and cellular response |
| title_full_unstemmed | Resveratrol-loaded PCL-PEG/GO/HAP biocomposite bone membranes: Evaluation of mechanical properties, release kinetics, and cellular response |
| title_short | Resveratrol-loaded PCL-PEG/GO/HAP biocomposite bone membranes: Evaluation of mechanical properties, release kinetics, and cellular response |
| title_sort | resveratrol loaded pcl peg go hap biocomposite bone membranes evaluation of mechanical properties release kinetics and cellular response |
| url | https://doi.org/10.1177/22808000251314087 |
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