A Sensitive Validated Spectrophotometric Method for the Determination of Flucloxacillin Sodium
A simple and sensitive spectrophotometric method has been proposed for the determination of flucloxacillin sodium. The determination method is based on charge transfer complexation reaction of the drug with iodine in methanol-dichloromethane medium. The absorbance was measured at 362 nm against the...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2009/219430 |
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| author | R. Singh Gujral S. Manirul Haque P. Shanker |
| author_facet | R. Singh Gujral S. Manirul Haque P. Shanker |
| author_sort | R. Singh Gujral |
| collection | DOAJ |
| description | A simple and sensitive spectrophotometric method has been proposed for the determination of flucloxacillin sodium. The determination method is based on charge transfer complexation reaction of the drug with iodine in methanol-dichloromethane medium. The absorbance was measured at 362 nm against the reagent blank. Under optimized experimental conditions, Beer's law is obeyed in the concentration ranges 1-9 μg/mL for flucloxacillin. The method was validated for specificity, linearity, precision, accuracy. The degree of linearity of the calibration curves, the percent recoveries, limit of detection and quantitation for the spectrophotometric method were determined. No interferences could be observed from the additives commonly present in the pharmaceutical formulations. The method was successfully applied for in vitro determination of human urine samples with low RSD value. This is simple, specific, accurate and sensitive spectrophotometric method. |
| format | Article |
| id | doaj-art-79d4bb1efffb4efb9596ae25a3ea4354 |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-79d4bb1efffb4efb9596ae25a3ea43542025-02-03T01:11:40ZengWileyE-Journal of Chemistry0973-49452090-98102009-01-016S1S397S40510.1155/2009/219430A Sensitive Validated Spectrophotometric Method for the Determination of Flucloxacillin SodiumR. Singh Gujral0S. Manirul Haque1P. Shanker2Vardhman Chemtech Ltd, Nimbua, Dera Bassi, Mohali (Punjab), IndiaVardhman Chemtech Ltd, Nimbua, Dera Bassi, Mohali (Punjab), IndiaVardhman Chemtech Ltd, Nimbua, Dera Bassi, Mohali (Punjab), IndiaA simple and sensitive spectrophotometric method has been proposed for the determination of flucloxacillin sodium. The determination method is based on charge transfer complexation reaction of the drug with iodine in methanol-dichloromethane medium. The absorbance was measured at 362 nm against the reagent blank. Under optimized experimental conditions, Beer's law is obeyed in the concentration ranges 1-9 μg/mL for flucloxacillin. The method was validated for specificity, linearity, precision, accuracy. The degree of linearity of the calibration curves, the percent recoveries, limit of detection and quantitation for the spectrophotometric method were determined. No interferences could be observed from the additives commonly present in the pharmaceutical formulations. The method was successfully applied for in vitro determination of human urine samples with low RSD value. This is simple, specific, accurate and sensitive spectrophotometric method.http://dx.doi.org/10.1155/2009/219430 |
| spellingShingle | R. Singh Gujral S. Manirul Haque P. Shanker A Sensitive Validated Spectrophotometric Method for the Determination of Flucloxacillin Sodium E-Journal of Chemistry |
| title | A Sensitive Validated Spectrophotometric Method for the Determination of Flucloxacillin Sodium |
| title_full | A Sensitive Validated Spectrophotometric Method for the Determination of Flucloxacillin Sodium |
| title_fullStr | A Sensitive Validated Spectrophotometric Method for the Determination of Flucloxacillin Sodium |
| title_full_unstemmed | A Sensitive Validated Spectrophotometric Method for the Determination of Flucloxacillin Sodium |
| title_short | A Sensitive Validated Spectrophotometric Method for the Determination of Flucloxacillin Sodium |
| title_sort | sensitive validated spectrophotometric method for the determination of flucloxacillin sodium |
| url | http://dx.doi.org/10.1155/2009/219430 |
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