Potential Protection of Coeloglossum viride var. Bracteatum Extract against Oxidative Stress in Rat Cortical Neurons
The present study explored the neuroprotective effect of Coeloglossum viride var. bracteatum extract (CE) against oxidative stress in rat cortical neurons. The results demonstrated that administration of CE inhibited hydrogen peroxide-induced neurotoxicity tested by MTT, LDH release, and TUNEL assay...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/326570 |
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| author | Zhe Guo Rui-Yuan Pan Xiao-Yan Qin |
| author_facet | Zhe Guo Rui-Yuan Pan Xiao-Yan Qin |
| author_sort | Zhe Guo |
| collection | DOAJ |
| description | The present study explored the neuroprotective effect of Coeloglossum viride var. bracteatum extract (CE) against oxidative stress in rat cortical neurons. The results demonstrated that administration of CE inhibited hydrogen peroxide-induced neurotoxicity tested by MTT, LDH release, and TUNEL assays. We further found that CE inhibited the activation of caspase-3 (Csp3) induced by hydrogen peroxide. Moreover, CE was found to reverse the hydrogen peroxide-induced downregulation of active AKT and Bcl-2. We then showed that the neuroprotective effect of CE was blocked by adding the AKT inhibitor, Ly294002. Thus, our data strongly indicated that CE played a neuroprotective role against oxidative stress-induced neurotoxicity. |
| format | Article |
| id | doaj-art-79c45fe4ed964ce589ee6d6d737f3b8a |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-79c45fe4ed964ce589ee6d6d737f3b8a2025-02-03T01:21:19ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/326570326570Potential Protection of Coeloglossum viride var. Bracteatum Extract against Oxidative Stress in Rat Cortical NeuronsZhe Guo0Rui-Yuan Pan1Xiao-Yan Qin2Beijing Engineering Research Center of Food Environment and Health, Minzu University of China, Beijing 100081, ChinaBeijing Engineering Research Center of Food Environment and Health, Minzu University of China, Beijing 100081, ChinaBeijing Engineering Research Center of Food Environment and Health, Minzu University of China, Beijing 100081, ChinaThe present study explored the neuroprotective effect of Coeloglossum viride var. bracteatum extract (CE) against oxidative stress in rat cortical neurons. The results demonstrated that administration of CE inhibited hydrogen peroxide-induced neurotoxicity tested by MTT, LDH release, and TUNEL assays. We further found that CE inhibited the activation of caspase-3 (Csp3) induced by hydrogen peroxide. Moreover, CE was found to reverse the hydrogen peroxide-induced downregulation of active AKT and Bcl-2. We then showed that the neuroprotective effect of CE was blocked by adding the AKT inhibitor, Ly294002. Thus, our data strongly indicated that CE played a neuroprotective role against oxidative stress-induced neurotoxicity.http://dx.doi.org/10.1155/2013/326570 |
| spellingShingle | Zhe Guo Rui-Yuan Pan Xiao-Yan Qin Potential Protection of Coeloglossum viride var. Bracteatum Extract against Oxidative Stress in Rat Cortical Neurons Journal of Analytical Methods in Chemistry |
| title | Potential Protection of Coeloglossum viride var. Bracteatum Extract against Oxidative Stress in Rat Cortical Neurons |
| title_full | Potential Protection of Coeloglossum viride var. Bracteatum Extract against Oxidative Stress in Rat Cortical Neurons |
| title_fullStr | Potential Protection of Coeloglossum viride var. Bracteatum Extract against Oxidative Stress in Rat Cortical Neurons |
| title_full_unstemmed | Potential Protection of Coeloglossum viride var. Bracteatum Extract against Oxidative Stress in Rat Cortical Neurons |
| title_short | Potential Protection of Coeloglossum viride var. Bracteatum Extract against Oxidative Stress in Rat Cortical Neurons |
| title_sort | potential protection of coeloglossum viride var bracteatum extract against oxidative stress in rat cortical neurons |
| url | http://dx.doi.org/10.1155/2013/326570 |
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